Expression vector production and high-throughput cell screening

1. A kit comprising isolated PCR primers, wherein:

(a) a first isolated PCR primer copies a VH gene segment and comprises a sequence selected from SEQ ID NOs: 1-5 and 7-17,

(b) a second isolated PCR primer copies a Vλ gene segment and comprises SEQ ID NO: 30, and

(c) a further at least three isolated PCR primers which copy Vκ gene segments and comprise at least 3 sequences selected from SEQ ID NOs: 18, 20-23, 25 and 26;

wherein any of the first, second or further at least three isolated PCR primers comprise at least one nucleic acid sequence tag.

2. The kit according to claim 1 , wherein the first isolated PCR primer copies a VH segment and comprises SEQ ID NO: 9, and wherein the second isolated PCR primer copies a Vλ, gene segment and comprises SEQ ID NO: 30.

3. The kit according to claim 1 , wherein the kit comprises further isolated PCR primers, wherein the further isolated PCR primers copy VH and Vκ gene segments, and wherein each of the isolated PCR primers comprises a sequence selected from SEQ ID NOs: 9, 18, 20-23, 25, and 26.

4. The kit according to claim 1 , wherein the kit comprises further isolated PCR primers, wherein the further isolated PCR primers copy VH, Vκ and Vλ, gene segments and each of the isolated PCR primers comprises a sequence selected from SEQ ID NOs: 9, 18, 20-23, 25, 26, and 30, such that each of the sequences of SEQ ID NOs: 9, 18, 20-23, 25, 26, and 30 is present in the mixture.

5. The kit according to claim 1 , wherein the kit further comprises at least one further isolated PCR primer comprising a sequence, wherein the sequence comprises:

a. an antibody heavy chain constant region sequence;

b. an antibody kappa chain constant region sequence; or

c. an antibody lambda chain constant region sequence.

6. The kit according to claim 1 , wherein the kit comprises one or more further isolated PCR primer(s) independently selected from:

a. an antibody heavy chain constant region sequence of SEQ ID NO: 48;

b. an antibody heavy chain constant region sequence of SEQ ID NO: 49;

c. an antibody kappa chain constant region sequence of SEQ ID NO: 50;

d. an antibody kappa chain constant region sequence of SEQ ID NO: 51; and

e. an antibody lambda chain constant region sequence of SEQ ID NO: 52.

7. The kit according to claim 1 , wherein the nucleic acid sequence of the at least one tag comprises a fragment of the human cytomegalovirus promoter at least 15 nucleotides in length.

8. The kit according to claim 1 , wherein the nucleic acid sequence of the tag comprises a polyA sequence.

9. The kit according to claim 1 , comprising a PCR primer mixture comprising the first and second isolated PCR primers.

10. A kit comprising at least a first, second, third and fourth isolated PCR primers, wherein:

a. the first isolated PCR primer copies a VH gene segment and comprises a sequence selected from SEQ ID NOs: 1-5 and 7-17; and

b. each of the second, third and fourth isolated PCR primers copy Vκ gene segments and each of the second, third and fourth isolated PCR primers comprises a sequence selected from SEQ ID NOs: 18, 20-23, 25 and 26, and

wherein any of the first, second, third or fourth isolated PCR primers comprise at least one nucleic acid sequence tag.

11. The kit according to claim 10 ,

comprising a further isolated PCR primer, wherein the further isolated primer copies a Vλ, gene segment and comprises SEQ ID NO: 30.

12. The kit according to claim 10 , wherein the kit further comprises at least one further isolated PCR primer comprising a sequence, wherein the sequence is comprised by:

a. an antibody heavy chain constant region sequence;

b. an antibody kappa chain constant region sequence; or

c. an antibody lambda chain constant region sequence.

13. The kit according to claim 10 , wherein the kit comprises one or more further isolated PCR primer(s) independently selected from:

a. an antibody heavy chain constant region sequence of SEQ ID NO: 48;

b. an antibody heavy chain constant region sequence of SEQ ID NO: 49;

c. an antibody kappa chain constant region sequence of SEQ ID NO: 50;

d. an antibody kappa chain constant region sequence of SEQ ID NO: 51; and

e. an antibody lambda chain constant region sequence of SEQ ID NO: 52.

14. A kit comprising at least a first isolated PCR primer and a different second isolated PCR primer, wherein the first isolated PCR primer copies a VH gene segment and comprises, from 5′ to 3′:

1) at least one nucleic acid sequence tag, and

2) a sequence selected from SEQ ID NOs: 1-17; and

the second isolated PCR primer copies a Vκ or Vλ, gene segment and comprises, from 5′ to 3′:

1) at least one nucleic acid sequence tag, and

2) a sequence selected from SEQ ID NOs: 18-47,

wherein the kit further comprises:

a. at least 3 further isolated PCR primers, wherein the at least 3 further isolated PCR primers copy VH gene segments, and wherein each further isolated PCR primer comprises a sequence selected from SEQ ID NOs: 1-17;

b. at least 3 further isolated PCR primers, wherein the at least 3 further isolated PCR primers copy Vκ gene segments, and wherein each further isolated PCR primer comprises a sequence selected from SEQ ID NOs: 18-26;

c. at least 3 further isolated PCR primers, wherein the at least 3 further isolated PCR primers copy Vλ, gene segments, and wherein each further isolated PCR primer comprises a sequence selected from SEQ ID NOs: 27-47;

d. at least 3 further isolated PCR primers, wherein the at least 3 further isolated PCR primers copy VH and Vκ gene segments, and wherein each further isolated PCR primer comprises a sequence selected from SEQ ID NOs: 1-26;

e. at least 3 further isolated PCR primers, wherein the at least 3 further isolated PCR primers copy VH and Vλ, gene segments, and wherein each further isolated PCR primer comprises a sequence selected from SEQ ID NOs: 1-17 and 27-47;

f. at least 3 further isolated PCR primers, wherein the at least 3 further isolated PCR primers copy VH, Vκ and Vκ gene segments, and wherein each further isolated PCR primer comprises a sequence selected from SEQ ID NOs: 1-47;

g. at least 3 further isolated PCR primers which copy VH gene segments and at least 3 further isolated PCR primers which copy Vκ gene segments, wherein each further isolated PCR primer comprises a sequence selected from SEQ ID NOs: 1-26;

h. at least 3 further isolated PCR primers which copy VH gene segments and at least 3 further isolated PCR primers which copy Vλ, gene segments, wherein each further isolated PCR primer comprises a sequence selected from SEQ ID NOs: 1-17 and 27-47; or

i. at least 3 further isolated PCR primers which copy VH gene segments, at least 3 further isolated PCR primers which copy Vκ gene segments, and at least 3 isolated PCR primers which copy Vλ, gene segments, wherein each further isolated PCR primer comprises a sequence selected from SEQ ID NOs: 1-47.

15. The kit according to claim 14 , wherein the kit further comprises at least one further isolated PCR primer comprising a sequence, wherein the sequence is comprised by:

a. an antibody heavy chain constant region sequence;

b. an antibody kappa chain constant region sequence; or

c. an antibody lambda chain constant region sequence.

16. The kit according to claim 14 , wherein the kit comprises one or more further isolated PCR primer(s) independently selected from:

a. an antibody heavy chain constant region sequence of SEQ ID NO: 48;

b. an antibody heavy chain constant region sequence of SEQ ID NO: 49;

c. an antibody kappa chain constant region sequence of SEQ ID NO: 50;

d. an antibody kappa chain constant region sequence of SEQ ID NO: 51; and

e. an antibody lambda chain constant region sequence of SEQ ID NO: 52.

17. A kit comprising a PCR primer mixture comprising at least a first isolated PCR primer and a second isolated PCR primer and a third isolated PCR primer, wherein the at least first, second and third isolated PCR primers are a) different, b) each comprise at least one nucleic acid sequence tag, and c) each PCR primer comprises a sequence selected from SEQ ID NOs: 18-26.

18. The kit according to claim 17 , wherein the kit further comprises at least one further isolated PCR primer comprising a sequence, wherein the sequence is comprised by:

a. an antibody heavy chain constant region sequence;

b. an antibody kappa chain constant region sequence; or

c. an antibody lambda chain constant region sequence.

19. The kit according to claim 17 , wherein the kit comprises one or more further isolated PCR primer(s) independently selected from:

a. an antibody heavy chain constant region sequence of SEQ ID NO: 48;

b. an antibody heavy chain constant region sequence of SEQ ID NO: 49;

c. an antibody kappa chain constant region sequence of SEQ ID NO: 50;

d. an antibody kappa chain constant region sequence of SEQ ID NO: 51; and

e. an antibody lambda chain constant region sequence of SEQ ID NO: 52.