Granted Patent B2

US 10,793,899 · App. 16/411,998 · Granted Oct 6, 2020

Inventors: Anjana Rao (La Jolla, CA); Mamta Tahiliani (New York, NY); Kian Peng Koh (Jamaica Plain, MA); Suneet Agarwal (Belmont, MA); Aravind Iyer (Bethesda, MD)

Assignees: CHILDREN'S MEDICAL CENTER CORPORATION; THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES

C12Q1/6827C12Q1/26C12Q1/6806C12Q1/6869G01N33/5308C12N2501/70C12N2501/71C12Q2521/531C12Q2522/10C12Q2537/164C12Q2600/154

Abstract

Claims (10)

1. A method comprising: performing an imaging method to detect a moiety associated with a glucosylated 5-hydroxymethylated cytosine in a nucleic acid that is from an extracellular fluid sample.

2. The method of claim 1 , wherein said moiety comprises a fluorophore.

3. The method of claim 1 , wherein said moiety comprises a biotinyl group.

4. The method of claim 1 , wherein said imaging method detects a presence of said glucosylated 5-hydroxymethylated cytosine in said nucleic acid.

5. The method of claim 1 , further comprising isolating said glucosylated 5-hydroxymethylated cytosine by affinity purification.

6. The method of claim 1 , wherein said nucleic acid is from a subject having cancer or suspected of having cancer.

7. An imager comprising a nucleic acid or an amplicon thereof, wherein said nucleic acid or said amplicon thereof comprises a glucosylated 5-hydroxymethylated cytosine, and wherein the nucleic acid is from an extracellular fluid sample.

8. The imager of claim 7 , wherein said glucosylated 5-hydroxymethylated cytosine is associated with a moiety.

9. The imager of claim 8 , wherein said moiety comprises a fluorophore.

10. The imager of claim 8 , wherein said moiety comprises a biotinyl group.

Assignments (5)

Continuity (9)

Cited By (5)

US 12,291,742 US 12,331,346 US 12,338,489 US 12,467,082 US 12,630,866