IP Library Granted Patent US 11,331,660
Granted Patent B2
US 11,331,660 · App. 16/416,352 · Granted May 17, 2022

Digital dispense system

Inventors: Bruce A. Deboard (Lexington, KY); John Glenn Edelen (Lexington, KY); Michael A. Marra, III (Lexington, KY); Sam Norasak (Lexington, KY)
B01L3/0241B01L3/502784G01N35/028B01J2219/00315B01L3/5085B01L2400/04
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Quick Facts
Patent No.
US 11,331,660
App. No.
16/416,352
Granted
May 17, 2022
Kind
B2
Abstract

A digital dispense system for preparing and analyzing a plurality of samples. The system includes two or more fluid droplet ejection devices. Each fluid droplet ejection device contains a fluid droplet ejection cartridge containing at least one fluid to be dispensed. The fluid droplet ejection cartridge is attached to a translation mechanism for moving the fluid droplet ejection cartridge back and forth over a sample holder in an x direction. A sample tray translation mechanism is provided for moving a sample tray along a production path in a y direction orthogonal to the x direction through the two or more fluid droplet ejection devices.

Claims (28)

1. A digital dispense system for preparing and analyzing a plurality of samples, comprising:

two or more rectangular-prism-shaped boxes disposed in series along a production path, each of the two or more rectangular-prism-shaped boxes containing a fluid droplet ejection cartridge containing at least one fluid to be dispensed, and a translation mechanism that is configured to move the fluid droplet ejection cartridge back and forth over a sample holder only in an x direction within each of the two or more rectangular-prism-shaped boxes, and

a sample tray translation mechanism that is configured to move a sample tray along the production path in only a y direction orthogonal to the x direction from a starting position to a terminal position through each of the two or more rectangular-prism-shaped boxes in series.

2. The digital dispense system of claim 1 , wherein the two or more rectangular-prism-shaped boxes are mechanically connected to one another, further comprising at least one clamp configured to clamp the two or more adjacent rectangular-prism-shaped boxes to one another in series along the production path.

3. The digital dispense system of claim 2 , wherein each of the two or more rectangular-prism-shaped boxes is electrically connected to one another using a male connector on a first rectangular-prism-shaped box and female connector on an adjacent rectangular-prism-shaped box configured to provide logic information transfer between the two or more interconnected rectangular-prism-shaped boxes.

4. The digital dispense system of claim 1 , wherein the sample tray translation mechanism comprises at least one track edge containing triangular teeth configured to intermesh with a gear of a stepping motor.

5. The digital dispense system of claim 1 , wherein the sample tray moves between a pair of guidance tracks attached to the two or more rectangular-prism-shaped boxes configured to accurately guide the sample tray along the production path through the two or more rectangular-prism-shaped boxes.

6. The digital dispense system of claim 1 , wherein each of the two or more rectangular-prism-shaped boxes contains a wireless transmitter and receiver configured to provide logic information transfer between the two or more rectangular-prism-shaped boxes.

7. The digital dispense system of claim 1 , further comprising sensors along the production path configured to index the tray position as it moves through the two or more rectangular-prism-shaped boxes.

8. The digital dispense system of claim 1 , further comprising a memory, a processor, and an input device, configured to control fluid droplet ejection from the fluid droplet ejection cartridge.

9. A method for preparing and analyzing a plurality of samples in a micro-well plate, comprising:

providing a digital dispense system disposed in series along a production path for preparing and analyzing the plurality of samples, the digital dispense system comprising two or more rectangular-prism-shaped boxes, each rectangular-prism-shaped box containing a fluid droplet ejection cartridge containing at least one fluid to be dispensed, and a translation mechanism for moving the fluid droplet ejection cartridge back and forth over a sample holder only in an x direction, and

providing a sample tray translation mechanism for moving a sample tray containing a plurality of sample wells along the production path only in a y direction orthogonal to the x direction from a starting position to a terminal position through each of the two or more rectangular-prism-shaped boxes;

moving the sample tray along the production path through the two or more rectangular-prism-shaped boxes; and

depositing fluid into the sample wells on the sample tray as the sample tray is moving through the two or more rectangular-prism-shaped boxes in series.

10. The method of claim 9 further comprising programming the two or more rectangular-prism-shaped boxes to deposit fluid into the plurality of sample wells on the sample tray as the sample tray moves along the production path.

11. The method of claim 9 , wherein only one fluid is deposited from each of the two or more rectangular-prism-shaped boxes into the sample wells as the sample tray moves along the production path.

12. The method of claim 9 , wherein the sample tray is moved sequentially along the production path from a first end to a second end of the production path.

13. The method of claim 9 , wherein the digital dispense system includes a memory, a processor, and an input device, further comprising: (a) inputting a droplet amount and droplet locations on the sample tray to the memory using the input device, (b) activating the processor to eject first fluid droplets to the droplet locations within a first rectangular-prism-shaped box, (c) indexing the sample tray along the production path to a second fluid droplet, (d) activating the processor to eject second fluid droplets to the droplet locations within a second rectangular-prism-shaped box, (e) repeating steps (b)-(d) for each of the two or more rectangular-prism-shaped boxes along the production path, and (f) terminating the droplet ejection procedure when the tray reaches a terminal end of the production path.

14. A portable sample analysis laboratory comprising a glove box enclosure containing a digital dispense system and a bag sealer configured for micro-well plate process and analysis, the digital dispense system comprising:

two or more interconnected rectangular-prism-shaped boxes connected to one another in series along a production path, each rectangular-prism-shaped box containing a fluid droplet ejection cartridge containing at least one fluid to be dispensed, and a translation mechanism configured to move the fluid droplet ejection cartridge back and forth over a sample holder in only an x direction, and

a sample tray translation mechanism configured to move a sample tray along the production path only in a y direction orthogonal to the x direction from a starting position to a terminal position through the two or more interconnected rectangular-prism-shaped boxes.

15. The portable sample analysis laboratory of claim 14 , further comprising at least one clamp configured to clamp the two or more adjacent rectangular-prism-shaped boxes to one another in series along the production path.

16. The portable sample analysis laboratory of claim 14 , wherein the sample tray translation mechanism comprises at least one track edge containing triangular teeth configured to intermesh with a gear of a stepping motor.

17. The portable sample analysis laboratory of claim 14 , wherein the sample tray moves between a pair of guidance tracks attached to the two or more interconnected rectangular-prism-shaped boxes wherein the pair of guidance tracks are configured to accurately guide the sample tray along the production path through the two or more interconnected rectangular-prism-shaped boxes.

18. The portable sample analysis laboratory of claim 14 , wherein each of the two or more interconnected rectangular-prism-shaped boxes is electrically connected using a male connector on a first rectangular-prism-shaped box and female connector on an adjacent rectangular-prism-shaped box configured to provide logic information transfer between the two or more interconnected rectangular-prism-shaped boxes.

19. The portable sample analysis laboratory of claim 14 , wherein each of the two or more interconnected rectangular-prism-shaped boxes contains a wireless transmitter and receiver configured to provide logic information transfer between the two or more interconnected rectangular-prism-shaped boxes.

20. The portable sample analysis laboratory of claim 14 , further comprising sensors along the production path configured to index the tray position as it moves through the two or more interconnected rectangular-prism-shaped boxes.

Assignments (2)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Apr 3, 2025
From: FUNAI ELECTRIC CO., LTD.
To: BRADY WORLDWIDE, INC.
Reel/Frame 070724/0730 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded May 20, 2019
From: DEBOARD, BRUCE A; EDELEN, JOHN GLENN; MARRA, MICHAEL A, III; NORASAK, SAM
To: FUNAI ELECTRIC CO. LTD
Reel/Frame 049222/0306 →
Continuity (2)
Provisional Application 62788290 · Jan 4, 2019
Related Publication 20200215531A1 · Jul 9, 2020