Treatment of
Provided herein are compositions and methods for the treatment or prevention of pathogenic infections.
1. A method to suppress an abnormal or excessive immune response comprising administering a pharmaceutical composition comprising a purified bacterial mixture consisting of 7 or more bacterial strains comprising 16S rDNA sequences of at least 97% sequence identity to nucleic acid sequences selected from the group consisting of SEQ ID NO: 10, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 19, SEQ ID NO: 20, and SEQ ID NO: 21, to the subject in an amount sufficient to suppress the abnormal or excessive immune response.
2. The method of claim 1 , wherein the abnormal or excessive immune response is suppressed by inducing proliferation and/or accumulation of regulatory T cells.
3. The method of claim 1 , wherein the subject is administered one or more doses of an antibiotic prior to the pharmaceutical composition.
4. The method of claim 3 , wherein the antibiotic is vancomycin, kanamycin, gentamicin, colistin, metronidazole, clindamycin, fidaxomicin, or cefoperazone.
5. The method of claim 3 , wherein the antibiotic is vancomycin.
6. The method of claim 1 , wherein the pharmaceutical composition further comprises a pharmaceutically acceptable excipient.
7. The method of claim 1 , wherein the pharmaceutical composition is formulated for oral administration.
8. The method of claim 1 , wherein the pharmaceutical composition is in the form of a capsule.
9. The method of claim 1 , wherein the pharmaceutical composition is formulated for delivery to the colon.
10. The method of claim 1 , wherein the pharmaceutical composition comprises between 1×10 7 and 1×10 10 colony forming units (CFUs) per bacterial strain.
11. A method to suppress an abnormal or excessive immune response comprising administering a pharmaceutical composition comprising a purified bacterial mixture consisting of 8 bacterial strains comprising 16S rDNA sequences of at least 97% sequence identity to SEQ ID NO: 10, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 19, SEQ ID NO: 20, and SEQ ID NO: 21, to the subject in an amount sufficient to suppress the abnormal or excessive immune response.
12. The method of claim 11 , wherein the abnormal or excessive immune response is suppressed by inducing proliferation and/or accumulation of regulatory T cells.
13. The method of claim 11 , wherein the subject is administered one or more doses of an antibiotic prior to the pharmaceutical composition.
14. The method of claim 13 , wherein the antibiotic is vancomycin, kanamycin, gentamicin, colistin, metronidazole, clindamycin, fidaxomicin, or cefoperazone.
15. The method of claim 13 , wherein the antibiotic is vancomycin.
16. The method of claim 11 , wherein the pharmaceutical composition further comprises a pharmaceutically acceptable excipient.
17. The method of claim 11 , wherein the pharmaceutical composition is formulated for oral administration.
18. The method of claim 11 , wherein the pharmaceutical composition is in the form of a capsule.
19. The method of claim 11 , wherein the pharmaceutical composition is formulated for delivery to the colon.
20. The method of claim 11 , wherein the pharmaceutical composition comprises between 1×10 7 and 1×10 10 colony forming units (CFUs) per bacterial strain.