Observation apparatus and objective
View Patent ↗An observation apparatus includes: alight source that emits pulsed light; and an objective that includes a first optical element serving as a light guide part and irradiates a sample with the pulsed light. The first optical element consists of a medium that satisfies the following conditional expression for θ1 and θ2: 0.75<θ2/θ1<1.33 where θ1=(n2−n1)/(λ2−λ1) and θ2=(n3−n2)/(λ3−λ2) are satisfied (λ1 indicates a light wavelength of 706.52 nm; λ2 indicates a light wavelength of 1529.6 nm; λ3 indicates a light wavelength of 2325.4 nm; and n1, n2, and n3 respectively indicate refractive indexes that the medium has for λ1, λ2, and λ3).
1. An observation apparatus comprising:
a light source that emits pulsed light; and
an objective that includes a first optical element serving as a light guide part and irradiates a sample with the pulsed light, wherein
the first optical element consists of a medium that satisfies the following conditional expression for θ1 and θ2:
0.75<θ2/θ1<1.33
where θ1=(n2−n1)/(λ2−λ1) and θ2=(n3−n2)/(λ3−λ2) are satisfied (λ1 indicates a light wavelength of 706.52 nm; λ2 indicates a light wavelength of 1529.6 nm; λ3 indicates a light wavelength of 2325.4 nm; and n1, n2, and n3 respectively indicate refractive indexes that the medium has for λ1, λ2, and λ3).
2. The observation apparatus of claim 1 , further comprising:
a scanner that performs scanning with the pulsed light; and
a second optical element disposed between the scanner and the objective, wherein
the second optical element consists of the medium that satisfies the conditional expression.
3. The observation apparatus of claim 2 , further comprising:
a third optical element different from the first and second optical elements that is disposed within the observation apparatus, wherein
the third optical element consists of the medium that satisfies the conditional expression.
4. The observation apparatus of claim 3 , wherein
the observation apparatus is a microscope.
5. The observation apparatus of claim 4 , wherein
the observation apparatus is a multiphoton excitation microscope.
6. The observation apparatus of claim 2 , wherein
the observation apparatus is a microscope.
7. The observation apparatus of claim 3 , wherein
the observation apparatus is a multiphoton excitation microscope.
8. The observation apparatus of claim 6 , wherein
the observation apparatus is a multiphoton excitation microscope.
9. The observation apparatus of claim 2 , wherein
the observation apparatus is a multiphoton excitation microscope.
10. The observation apparatus of claim 1 , wherein
the observation apparatus is a microscope.
11. The observation apparatus of claim 10 , wherein
the observation apparatus is a multiphoton excitation microscope.
12. The observation apparatus of claim 1 , wherein
the observation apparatus is a multiphoton excitation microscope.
13. An objective comprising:
an optical element serving as a light guide part, wherein
the optical element consists of a medium that satisfies the following conditional expression for θ1 and θ2:
0.75<θ2/θ1<1.33
where θ1=(n2−n1)/(λ2−λ1) and θ2=(n3−n2)/(λ3−λ2) are satisfied (λ1 indicates a light wavelength of 706.52 nm; λ2 indicates a light wavelength of 1529.6 nm; λ3 indicates a light wavelength of 2325.4 nm; and n1, n2, and n3 respectively indicate refractive indexes that the medium has for λ1, λ2, and λ3).