Sporicidal methods and compositions
Provided herein are methods and compositions for the reduction of endospore contamination of a substrate with percarboxylic acid-based compositions. The methods can include the step of continuously monitoring the oxidation-reduction potential of the percarboxylic acid-based compositions.
1. A method of treating a substrate for endospore contamination, the method comprising:
a) preparing a solution comprising peracetic acid and hydrogen peroxide at a weight percent ratio of between 1.2 and 3.8;
b) adjusting the concentration of the peracetic acid and hydrogen peroxide in the solution of step a) to provide an oxidation-reduction potential (ORP) of 500 mV to 1000 mV; and
c) contacting the substrate with the solution of step b) for a time sufficient to reduce the endospore contamination, wherein the ORP is continuously monitored during the contacting step and wherein the solution of step b is applied to the substrate as a liquid, mist or fog;
and wherein:
peracetic acid is added during the monitoring of step c) to maintain the ORP at 500 mV to 1000 mV without the addition of hydrogen peroxide; and
contact is maintained in step c) for up to 10 minutes.
2. The method of claim 1 , wherein the method is performed in the absence of a peroxide decomposing enzyme.
3. The method of claim 1 , wherein the endospores are produced by a microbe selected from the group consisting of Paenibacillus chibensis, Paenibacillus favisporus, Bacillus cereus, Bacillus atrophaeus, Bacillus subtilis, Geobacillus stearothermophilus and a combination thereof.
4. The method of claim 1 , wherein the substrate comprises a material selected from the group consisting of metal, plastic, ceramic, glass, wood, rubber composite, and a combination thereof.
5. The method of claim 1 , wherein the substrate comprises a medical device, food or beverage preparation equipment, pharmaceutical manufacturing equipment, or a foodstuff.
6. The method of claim 1 , wherein the contacting in step c) is for 3 to 60 seconds.
7. The method of claim 6 , wherein the solution of step a) comprises 500-7500 ppm of peracetic acid.
8. The method of claim 7 , wherein the contacting in step c) is for 3 to 60 seconds.
9. The method of claim 1 , wherein the contacting in step c comprises dipping, spraying, immersing or soaking.
10. The method of claim 1 , wherein the oxidation-reduction potential (ORP) of the solution of step c) is 540 mV to 780 mV and peracetic acid is added during the monitoring of step c) to maintain the ORP in this range.
11. The method of claim 1 , wherein the contacting in step c is for 30 seconds to 8 minutes.
12. The method of claim 1 , wherein the endospore contamination is reduced by about 1 log 10 .
13. The method of claim 1 , wherein the endospore contamination is reduced by about 90%.
14. The method of claim 3 , wherein the substrate comprises a material selected from the group consisting of metal, plastic, ceramic, glass, wood, rubber composite, and a combination thereof.
15. The method of claim 14 , wherein the substrate comprises a medical device, food or beverage preparation equipment, pharmaceutical manufacturing equipment, or a foodstuff.
16. The method of claim 15 , wherein the method is performed in the absence of a peroxide decomposing enzyme.
17. The method of claim 16 , wherein the contacting in step c) is for 3 to 60 seconds.
18. The method of claim 15 , wherein the contacting in step c) is for 3 to 60 seconds.
19. The method of claim 18 , wherein the contacting in step c comprises dipping, spraying, immersing or soaking.
20. The method of claim 1 , wherein the solution of step a), comprising peracetic acid and hydrogen peroxide, further comprises an adjuvant.