Detection of colorectal cancer
The present disclosure provides, among other things, methods for colorectal cancer screening and compositions related thereto. In various embodiments, the present disclosure provides methods for colorectal cancer screening that include analysis of methylation status of one or more methylation biomarkers, and compositions related thereto. In various embodiments, the present disclosure provides methods for colorectal cancer screening that include screening methylation status of one or more methylation biomarkers in cfDNA, e.g., in ctDNA. In various embodiments, the present disclosure provides methods for colorectal cancer screening that include screening methylation status of one or more methylation biomarkers in cfDNA, e.g., in ctDNA, using MSRE-qPCR.
1. A method of screening for colorectal cancer, the method comprising determining the methylation status of at least one DMR of PDGFD, at least one DMR of FGF14 and at least one DMR of ZNF471 in DNA of a human subject, and diagnosing colorectal cancer in the subject.
2. The method of claim 1 , the method comprising determining whether the one or more methylation loci are hypermethylated as compared to a reference, wherein hypermethylation is indicative of colorectal cancer.
3. The method of claim 1 , wherein the method comprises determining the methylation status of at least one DMR of ADAMTS2.
4. The method of claim 1 , wherein the method comprises determining the methylation status of at least one DMR of ADAMTS2, at least one DMR of ZNF492, and at least one DMR of ST6GALNAC5.
5. The method of claim 1 , wherein the method comprises determining the methylation status of at least one DMR of ADAMTS2, at least one DMR of ZNF492, at least one DMR of ST6GALNAC5, at least one DMR of ZNF542, at least one DMR of LONRF2, at least one DMR of ZNF132, at least one DMR of CNRIP1, and at least one DMR of ALK.
6. The method of claim 1 , wherein the DNA is isolated from blood, plasma, urine, saliva, or stool of the human subject.
7. The method of claim 1 , wherein the DNA is cell-free DNA of the human subject.
8. The method of claim 1 , wherein the method provides a sensitivity for colorectal cancer of at least 0.6.
9. The method of claim 1 , wherein the method provides a specificity for colorectal cancer of at least 0.7.
10. The method of claim 1 , wherein methylation status is determined using one or more members selected from the group consisting of methylation sensitive restriction enzyme quantitative polymerase chain reaction (MSRE-qPCR), Methylation-Specific PCR, Methylation Specific Nuclease-assisted Minor-allele Enrichment PCR, and next-generation sequencing.
11. The method of claim 1 , wherein the method comprises determining the methylation status of at least one DMR of LONRF2.