Demineralized bone matrix composition with enhanced osteoinductivity and osteoconductivity
An osteoinductive and osteoconductive composition including DBM fibers having multiple geometries, as well as a method of producing the same are disclose. For example, such a method may include: obtaining bone tissue; cleaning the bone tissue obtained with a plurality of washes; milling the cleaned bone tissue into a plurality of fibers of two or more geometries including a long wide fiber, a long narrow fiber, a short wide fiber, and a short narrow fiber; demineralizing the plurality of fibers of two or more geometries to expose one or more biologically active components; and neutralizing the plurality of demineralized fibers of two or more geometries resulting in demineralized bone matrix.
1. A method of producing an osteoinductive and osteoconductive composition for in vivo use, the method comprising:
obtaining bone tissue;
cleaning the bone tissue obtained with a plurality of washes;
milling the cleaned bone tissue into a plurality of fibers, wherein each fiber of the plurality of fibers has a geometry selected from:
a long wide fiber, wherein the long wide fiber has a length of about 10 mm or greater and a width between about 0.6 mm and about 1.0 mm,
a long narrow fiber, wherein the long narrow fiber has a length of greater than about 10 mm and a width between about 0.2 mm and about 0.6 mm,
a short wide fiber, wherein the short wide fiber has a length of less than about 10 mm and a width between about 0.6 mm and about 1.0 mm, or
a short narrow fiber, wherein the short narrow fiber has a length of less than about 10 mm and a width between about 0.2 mm and about 0.6 mm;
demineralizing the plurality of fibers to expose one or more biologically active components;
selecting a mixture of fibers from the plurality of fibers, wherein the mixture consists of a ratio of long wide fibers to long narrow fibers;
combining the mixture of fibers to yield an osteoinductive and osteoconductive composition having osteoinductive properties and osteoconductive properties, wherein:
the ratio comprises a larger proportion of long wide fibers to impart greater osteoinductive properties to the osteoinductive and osteoconductive composition, or
the ratio comprises a larger proportion of long narrow fibers to impart greater osteoconductive properties to the osteoinductive and osteoconductive composition.
2. The method of claim 1 , wherein the long wide fiber has a length between about 10 mm and about 50 mm, and a width of about 0.8 mm.
3. The method of claim 1 , wherein the long narrow fiber has a length between about 10 mm and about 50 mm and a width of about 0.4 mm.
4. The method of claim 1 , wherein the short wide fiber has a length of less than about 10 mm and a width of about 0.8 mm.
5. The method of claim 1 , wherein the short narrow fiber has a length of less than about 10 mm and a width of about 0.4 mm.
6. The method of claim 1 , wherein the ratio of the long wide fibers to the long narrow fibers is about 65:35.
7. The method of claim 1 , further comprising freeze-drying the plurality of demineralized fibers of two or more geometries.
8. The method of claim 1 , wherein demineralizing the plurality of fibers of two or more geometries includes treating the plurality of fibers with a hydrochloric acid treatment.
9. The method of claim 8 , further comprising neutralizing the plurality of demineralized fibers of two or more geometries resulting in demineralized bone matrix.