Method and apparatus for selecting slide media image read location
The present disclosure is directed to a method and apparatus for locating a target location on a reaction cell and using the target location to perform an assay. In an example embodiment, a method of performing at least one assay includes obtaining at least one image of a fluid sample located on a reaction cell and creating a set of derivative data including a plurality of derivative data points based on the at least one image. The method also includes determining an image gradient data point for each of the plurality of derivative data points and determining a target location of the fluid sample in the reaction cell based on the image gradient data points. The method further includes performing at least one assay using the target location of the fluid sample in the reaction cell.
1. A method of performing at least one assay comprising:
obtaining, in a control unit, an image of a fluid sample that is located on a reaction cell;
determining, via the control unit, a default center corresponding to at least one of a center of the reaction cell or a center of a dispense location of the fluid sample onto the reaction cell;
creating, via the control unit, a set of derivative data including a plurality of derivative data points based on the image;
determining, via the control unit, an image gradient data point for each of the plurality of derivative data points to create a binary image by:
comparing each of the plurality of derivative data points to a threshold value,
assigning a value of ‘0’ to the image gradient data points that correspond to the derivative data points that have a value that is greater than the threshold value to remove unwanted image artifacts, and
assigning a value of ‘1’ to the remaining image gradient data points;
determining, via the control unit, a center of mass of the binary image using the image gradient data points;
designating, via the control unit, the determined center of mass as a target location of the fluid sample on the reaction cell;
comparing, via the control unit, the target location to the default center; and
performing, via the control unit and a related assay system, the at least one assay using the target location of the fluid sample on the reaction cell when a distance between the default center and the target location is less than a threshold.
2. The method of claim 1 , wherein the reaction cell includes a solid media, a dry slide, or a reaction cuvette.
3. The method of claim 1 , wherein the target location includes a center of a homogeneous region of the fluid sample on the reaction cell.
4. The method of claim 1 , which includes performing multiple assays using target locations from a plurality of images.
5. The method of claim 1 , wherein the plurality of derivative data points are first order derivatives that are based on colors in the image.
6. The method of claim 1 , wherein determining the target location of the fluid sample includes at least one of:
(i) using first image gradient data points having a lower image gradient than second image gradient data points; or
(ii) excluding the second image gradient data points having a higher image gradient than the first image gradient data points.
7. The method of claim 1 , which includes removing, via the control unit, image defects from the image.
8. The method of claim 1 , wherein the image is a two-dimensional image.
9. The method of claim 1 , which includes forming, via the control unit, a read area around the target location, and wherein performing the at least one assay includes using the read area.
10. The method of claim 1 , wherein determining the target location of the fluid sample includes detecting or measuring an indicator reaction of indicator molecules on the reaction cell formed from at least one reagent combining with the fluid sample.
11. The method of claim 1 , further comprising reporting, via the control unit, an error indicative that a new fluid sample should be dispensed on a new reaction cell when the distance between the default center and the target location is greater than or equal to the threshold.
12. A method of performing at least one assay comprising:
obtaining, via a control unit, an image of a fluid sample located on a reaction cell;
determining, via the control unit, a default center corresponding to at least one of a center of the reaction cell or a center of a dispense location of the fluid sample onto the reaction cell;
creating, via the control unit, a set of derivative data including a plurality of derivative data points based on the image;
determining, via the control unit, an image gradient data point for each of the plurality of derivative data points to create a binary image by:
comparing each of the plurality of derivative data points to a threshold value,
assigning a value of ‘0’ to the image gradient data points that correspond to the derivative data points that have a value that is greater than the threshold value to remove unwanted image artifacts, and
assigning a value of ‘1’ to the remaining image gradient data points;
determining, via the control unit, a center of mass of the binary image using the image gradient data points;
designating, via the control unit, the determined center of mass as a target location of the fluid sample;
forming, via the control unit, a read area around the target location;
comparing, via the control unit, the target location to the default center; and
performing, via the control unit and a related assay system, the at least one assay using the read area when a distance between the default center and the target location is less than a threshold.
13. The method of claim 12 , wherein the reaction cell includes a solid media, a dry slide, or a reaction cuvette.
14. The method of claim 12 , wherein the read area appears circular in the image.
15. The method of claim 12 , wherein the read area appears elliptical in the image.
16. The method of claim 12 , which includes setting, via the control unit, each image gradient data point to a value of ‘1’ if the image gradient data point has a value that is less than the threshold value.
17. The method of claim 12 , which includes performing, via the control unit and the related assay system, multiple assays using target locations from a plurality of images.
18. The method of claim 12 , wherein the image is a two-dimensional image.
19. An apparatus for performing at least one assay comprising:
a slide reception location configured to receive at least one reaction cell having a fluid sample located thereon;
an imaging device positioned and arranged relative to the slide reception location to obtain an image of the fluid sample located on the reaction cell; and
a control unit configured to:
determine a default center corresponding to at least one of a center of the reaction cell or a center of a dispense location of the fluid sample onto the reaction cell,
create a set of derivative data including a plurality of derivative data points based on the image,
determine an image gradient data point for each of the plurality of derivative data points to create a binary image by:
comparing each of the plurality of derivative data points to a threshold value,
assigning a value of ‘0’ to the image gradient data points that correspond to the derivative data points that have a value that is greater than the threshold value to remove unwanted image artifacts, and
assigning a value of ‘1’ to the remaining image gradient data points,
determine a center of mass of the binary image using the image gradient data points,
designate the determined center of mass as a target location within the fluid sample located on the reaction cell, compare the target location to the default center, and
perform the at least one assay based on the target location when a distance between the default center and the target location is less than a threshold.
20. The apparatus of claim 19 , wherein the reaction cell includes a solid media, a dry slide, or a reaction cuvette.
21. The apparatus of claim 20 , wherein the solid media includes a second reaction cell.
22. The apparatus of claim 19 , wherein the target location includes a central location of a homogeneous region of the fluid sample.
23. The apparatus of claim 19 , wherein the control unit is configured to set each image gradient data point to a value of ‘1’ if the respective image gradient data point has a value that is less than the threshold value.
24. The apparatus of claim 19 , wherein the control unit is configured to determine the target location by at least one of: (i) using first image gradient data points having a lower image gradient than second image gradient data points; or (ii) excluding the second image gradient data points having a higher image gradient than the first image gradient data points.
25. The apparatus of claim 19 , wherein the control unit is configured to determine the target location at a geometrical center of first image gradient data points that have a lower image gradient than second image gradient data points.
26. The apparatus of claim 19 , wherein the control unit is configured to perform multiple different assays using target locations from a plurality of images.
27. The apparatus of claim 19 , wherein the target location of the fluid sample on the reaction cell corresponds to a location where an indicator reaction occurs such that an indicator is developed for display as target molecules in the fluid sample react with reagents on the reaction cell.