Method and system for sample preparation
A method for preparing a sample by utilizing a shearing force in the presence of a size stabilizer to break apart the sample to obtain nucleic acid molecules in a usable size range. Once nucleic acid molecules are obtained, magnetic nanoparticles are used to concentrate and clean the nucleic acid molecules for further testing.
1. A disposable cartridge for preparing an assay fluid, comprising:
a cartridge body including (i) a cylindrical surface defining a fixed port and (ii) a syringe barrel defining a bore for receiving a moveable plunger, the fixed port disposed in fluid communication with the bore of the syringe barrel; and
a rotor mounting to the cartridge body and including a surface slideably engaging the cylindrical surface of the cartridge body along a mating interface, the rotor including:
a plurality of chambers each configured to facilitate at least one operation on the assay fluid, and
a plurality of moveable ports disposed along the surface of the rotor and in fluid communication with at least one of the plurality of chambers;
wherein the rotor is rotationally indexed about an axis parallel to the cylindrical surface such that the plurality of moveable ports are selectively aligned with the fixed port of the cartridge body.
2. The disposable cartridge of claim 1 wherein the moveable plunger is caused to translate within the bore to effect a flow of assay fluids from one chamber to another chamber to perform the at least one operation on the assay fluid.
3. The disposable cartridge of claim 1 wherein the cylindrical surface of the cartridge body includes at least one surface to close at least one of the plurality of moveable ports.
4. The disposable cartridge of claim 2 wherein the rotor further comprises a reaction chamber in fluid communication with one of the plurality of moveable ports.
5. The disposable cartridge of claim 4 wherein the at least one of the plurality of moveable ports is indexed to one of a plurality of radial positions each corresponding to alignment with the fixed port of the cartridge body and with a variety of other chambers of the rotor so that the assay fluid can be: (i) drawn into the syringe barrel, (ii) injected into one of the other chambers containing at least one processing compound, and (iii) injected into the reaction chamber for evaluating a test result associated with the assay fluid.
6. The disposable cartridge of claim 2 wherein the at least one of the plurality of moveable ports is indexed: (i) to a first radial position corresponding to alignment with the fixed port of the cartridge body, (ii) to another radial position corresponding to a reaction chamber of the cartridge body.
7. The disposable cartridge of claim 1 , wherein the assay fluid travels through at least one chamber formed along a bottom panel of the rotor to provide a passageway for fluid flow from one chamber to another chamber.
8. The disposable cartridge of claim 4 , wherein the assay fluid travels through chambers formed along a bottom panel of the rotor to provide a passageway for fluid flow from one chamber to the reaction chamber.
9. The disposable cartridge of claim 8 , further comprising a signal processor disposed in the reaction chamber to detect the presence of a nucleic acid sequence.
10. The disposable cartridge of claim 2 , wherein at least one of a plurality of ports is disposed at a predetermined height from the bottom panel of the rotor to prevent a backflow of assay fluid into one of the chambers.
11. The disposable cartridge of claim 2 , further comprising a washing channel disposed along the bottom panel of the rotor, the washing channel including a first portion defining a first width dimension and a second portion defining a second width dimension which is greater than the first width, the second portion of the washing channel configured to concentrate magnetic nanoparticles.
12. The disposable cartridge of claim 1 , wherein one of the rotor and the cartridge body includes an identifying marker from the group of: a barcode label and a Radio Frequency Identification (RFID) tag.
13. The disposable cartridge of claim 1 , further comprising a chip including a biological probe.
14. The disposable cartridge of claim 1 wherein the chambers of the rotor have at least one open end defining an upper rim, and further comprising a thin film disposed over, and heat sealed to, the upper rim.
15. A method for preparing an assay fluid, including the steps of:
configuring a cartridge body with: (i) a cylindrical surface defining a fixed port and (ii) a syringe barrel defining a bore for receiving a moveable plunger, the fixed port disposed in fluid communication with the bore of the syringe barrel;
configuring a rotor for mounting to the cartridge body and including (i) a cylindrical surface for slideably engaging the cylindrical surface of the cartridge body along a mating interface, (ii) a plurality of chambers, and (iii) a plurality of movable ports disposed in the cylindrical surface and in fluid communications with the plurality of chambers;
rotationally indexing the rotor about an axis parallel to the cylindrical surface of the cartridge body; and
displacing the moveable plunger in the syringe barrel to cause the assay fluid to translate from the fixed port to one or more of the moveable ports, the assay fluid being moved from the chambers of the rotor to a reaction chamber where properties of the assay fluid are evaluated.
16. The method of claim 15 wherein a printed circuit board within the reaction chamber detects for the presence of a nucleic acid sequence.
17. The method of claim 15 comprising the steps of configuring the rotor with a washing channel along a bottom panel of the rotor such that the washing channel includes a first portion defining a first width dimension and a second portion defining a second width dimension which is greater than the first width dimension, and concentrating magnetic nanoparticles in the washing channel to prepare the assay fluid.
18. The method of claim 15 , including the step of configuring the rotor with an identifying marker.
19. The method of claim 18 wherein the identifying marker is a barcode label.
20. The method of claim 18 wherein the identifying marker is a Radio Frequency Identification (RFID) tag.