Method of making osteoinductive bone implant
Disclosed are various bioactive grafts and methods of making the same. In one embodiment, bone material is harvested from a donor. The harvested bone material is exposed to a lysing agent, the lysing agent configured to release growth factors and bioactive materials from cellular material of the harvested bone material. The harvested bone material is then rinsed with a rinsing agent. The pH of the harvested bone material is substantially neutralized.
1. A bone implant prepared by a process comprising the steps of:
harvesting bone material from a donor;
exposing the harvested bone material to a lysing agent, the lysing agent configured to release native bone morphogenetic proteins from the harvested bone material;
allowing the released native bone morphogenetic proteins to bind to the harvested bone material;
demineralizing the harvested bone with bone morphogenetic proteins bound thereto with hydrochloric acid;
rinsing the harvested bone material;
wherein the rinsed bone comprises a bone implant comprising demineralized bone and bone morphogenetic proteins derived from the bone.
2. The bone implant of claim 1 , wherein the harvested bone material comprises bone marrow.
3. The bone implant of claim 1 , wherein the harvested bone comprises cortical or cancellous bone.
4. The bone implant of claim 1 , wherein the bone comprises ground particulate.
5. The bone implant of claim 1 , wherein the implant further comprises antibiotics.
6. The bone implant of claim 1 , having reduced bone modulus.