LIPOSOMAL ELINAFIDE FORMULATIONS AND USES THEREOF
The present invention provides compositions for the treatment of cancer. The compositions include liposomes containing a phosphatidylcholine lipid, a sterol, a PEG-lipid, and elinafide, wherein less than 20% of the elinafide is released in vitro from the liposome within 40 hours. The present invention also provides liposomal compositions for the treatment of cancer comprising administering to a patient in need thereof a liposome, wherein the liposome comprises: a phosphatidylcholine lipid; a sterol; a PEG-lipid; and elinafide, wherein the administration of the liposomal composition has a reduced occurrence of side effects, such as for example, muscle myopathy in the patient.
1 . A liposomal composition for the treatment of cancer in a patient in need thereof comprising:
a) a liposome comprising:
i) about 50 mol % to about 70 mol % of a phosphatidylcholine lipid or mixture of phosphatidylcholine lipids;
ii) about 25 mol % to about 45 mol % of cholesterol;
iii) about 2 mol % to about 8 mol % of a PEG-lipid; and
iv) elinafide; and
b) a pharmaceutically acceptable excipient.
2 . The liposomal composition of claim 1 , wherein the phosphatidylcholine lipid is selected from the group consisting of: palmitoyloleoylphosphatidylcholine (POPC), distearoylphosphatidylcholine (DSPC), dimyristoylphosphatidylcholine (DMPC), dipalmitoylphosphatidylcholine (DPPC), hydrogenated soy phosphatidylcholine (HSPC), 1,2-diarachidoyl-sn-glycero-3-phosphocholine, and mixtures thereof.
3 . The liposomal composition of claim 2 , wherein the phosphatidylcholine lipid is DSPC.
4 . The liposomal composition of claim 1 , wherein the PEG-lipid is selected from the group consisting of distearoyl-phosphatidylethanolamine-N-[methoxy(polyethene glycol)-2000](DSPE-PEG-2000) and distearoyl-phosphatidylethanolamine-N-[methoxy(polyethene glycol)-5000] (DSPE-PEG-5000).
5 . The liposomal composition of claim 1 , wherein the liposome comprises about 55% of DSPC; about 40% cholesterol; and about 5% DSPE-PEG-2000.
6 . The liposomal composition of claim 1 , wherein the ratio of the total lipid weight to the elinafide is about 1:0.1 to 1:1.
7 . The liposomal composition of claim 1 , wherein the liposome has a volume mean particle size of about 125 nm or less.
8 . The liposomal composition of claim 7 , wherein the liposome has a volume mean particle size of about 50 nm about 100 nm.
9 . The liposomal composition of claim 1 , wherein less than 10% of the elinafide is released from the liposome within 20 hours.
10 . The liposomal composition of claim 1 , wherein the composition has an IC 50 of about 10.0 μM or less after 2 hours in HT29 cells.
11 . The liposomal composition of claim 1 , wherein the composition has an IC 50 of about 1.0 μM or less after 24 hours in HT29 cells.
12 . The liposomal composition of claim 1 , wherein the composition has a plasma AUC greater than free elinafide or a clearance rate (Cl) less than free elinafide.
13 . The liposomal composition of claim 1 , wherein composition inhibited tumor growth greater than free elinafide.
14 . The liposomal composition of claim 1 , wherein the composition delayed tumor growth greater than free elinafide.
15 . The liposomal composition of claim 1 , wherein the composition does not induce or has reduced muscle myopathy compared to free elinafide.
16 . A method of treating cancer in a patient in need thereof, comprising the steps of:
(a) administering to the patient in need thereof a liposomal composition comprising
i) a liposome comprising:
1) about 50 mol % to about 70 mol % of a phosphatidylcholine lipid or mixture of phosphatidylcholine lipids;
2) about 25 mol % to about 45 mol % of cholesterol;
3) about 2 mol % to about 8 mol % of a PEG-lipid; and
4) elinafide; and
ii) a pharmaceutically acceptable excipient;
wherein the administration of the liposomal composition has a reduced occurrence of muscle myopathy in the patient.
17 . The method of treating cancer of claim 16 , wherein an initial dose of the liposomal composition is from about 0.001 mg/kg to about 1000 mg/kg daily.
18 . The method of treating cancer of claim 17 , wherein the initial dose of the liposomal composition is from about 1 g/kg to about 100 mg/kg daily.
19 . The method of treating cancer of claim 16 , wherein a daily dose of the liposomal composition is 3, 6, 12, 24, 48, 80, 120, 160, 190, 225, 270, or 320 mg/M 2 .