Amphiphilic block copolymer composition having enhanced micelle stability, and pharmaceutical composition comprising same
The present invention relates to an amphiphilic block copolymer composition which provides enhanced stability to a micelle formed by an amphiphilic block copolymer in an aqueous phase.
1. A method for purifying an amphiphilic block copolymer composition, comprising:
(a) a step of dissolving an amphiphilic block copolymer composition comprising a hydrophilic block (A) and a hydrophobic block (B) in an organic solvent selected from the group consisting of ethanol, methanol, isopropyl alcohol and mixtures thereof at a temperature of 40° C. to 70° C. for 1 hour to 6 hours; and
(b) a step of layer separation of the resulting solution from step (a) at a temperature of 15° C. to 35° C. for 1 hour to 6 hours,
wherein the amphiphilic block copolymer composition satisfies the conditions of the following Equation 1:
Area of peak ( a )/[Area of peak ( a )+Area of peak ( b )]×100≥85 [Equation 1]
wherein peak (a) is a peak indicated by a micelle polymer in size exclusion chromatogram, and
peak (b) is a peak indicated by a non-micelle polymer in size exclusion chromatogram.
2. The method for purifying an amphiphilic block copolymer composition according to claim 1 , wherein the organic solvent used in step (a) is in an amount of 2 ml/g to 8 ml/g based on the weight of the amphiphilic block copolymer composition.
3. The method for purifying an amphiphilic block copolymer composition according to claim 1 , wherein the organic solvent used in step (a) is ethanol.
4. The method for purifying an amphiphilic block copolymer composition according to claim 1 , further comprising a step of removing remaining organic solvent from the result of step (b).
5. The method for purifying an amphiphilic block copolymer composition according to claim 1 , wherein the amphiphilic block copolymer composition satisfies the conditions of the following Equation 1a:
Area of peak ( a )/[Area of peak ( a )+Area of peak ( b )]×100≥90 [Equation 1a]
wherein peak (a) is a peak indicated by a micelle polymer in size exclusion chromatogram, and
peak (b) is a peak indicated by a non-micelle polymer in size exclusion chromatogram.
6. The method for purifying an amphiphilic block copolymer composition according to claim 1 , wherein the amphiphilic block copolymer composition satisfies the conditions of the following Equation 1b:
Area of peak ( a )/[Area of peak ( a )+Area of peak ( b )]×100≥95 [Equation 1b]
wherein peak (a) is a peak indicated by a micelle polymer in size exclusion chromatogram, and
peak (b) is a peak indicated by a non-micelle polymer in size exclusion chromatogram.
7. The method for purifying an amphiphilic block copolymer composition according to claim 1 , wherein the amphiphilic block copolymer composition satisfies the conditions of the following Equation 2:
Area of peak ( a )/[Area of peak ( a )+Area of peak ( b )+Area of peak ( c )]×100≥85 [Equation 2]
wherein peak (a) is a peak indicated by a micelle polymer in size exclusion chromatogram,
peak (b) is a peak indicated by a non-micelle polymer in size exclusion chromatogram, and
peak (c) is a peak indicated by a monomer in size exclusion chromatogram.
8. The method for purifying an amphiphilic block copolymer composition according to claim 1 , wherein the amphiphilic block copolymer composition satisfies the conditions of the following Equation 2a:
Area of peak ( a )/[Area of peak ( a )+Area of peak ( b )+Area of peak ( c )]×100≥90 [Equation 2a]
wherein peak (a) is a peak indicated by a micelle polymer in size exclusion chromatogram,
peak (b) is a peak indicated by a non-micelle polymer in size exclusion chromatogram, and
peak (c) is a peak indicated by a monomer in size exclusion chromatogram.
9. The method for purifying an amphiphilic block copolymer composition according to claim 1 , wherein the amphiphilic block copolymer composition satisfies the conditions of the following Equation 2b:
Area of peak ( a )/[Area of peak ( a )+Area of peak ( b )+Area of peak ( c )]×100≥95 [Equation 2b]
wherein peak (a) is a peak indicated by a micelle polymer in size exclusion chromatogram,
peak (b) is a peak indicated by a non-micelle polymer in size exclusion chromatogram, and
peak (c) is a peak indicated by a monomer in size exclusion chromatogram.