IP Library › Granted Patent US 11,555,202

Granted Patent B2

US 11,555,202 · App. 16/471,776 · Granted Jan 17, 2023

Elite event EE-GM5 and methods and kits for identifying such event in biological samples

Inventors: Hal Moser (Shafter, CA); Michael McCarville (Omaha, NE); Maxim Buyse (Zwijnaarde, BE); Filip Slabbinck (Zwijnaarde, BE); Vadim Beilinson (Cary, NC); Thomas W Kleven (Muskegon, MI); Julia Daum (Morrisville, NC); Wendy Aartsen (Zwijnaarde, BE); Veerle Habex (Zwijnaarde, BE)

Assignee: BASF AGRICULTURAL SOLUTIONS SEED US LLC

C12N15/8274 A01N35/06 A01N43/80 C12N15/11 C12N15/8285 C12Q1/6895 C12Q2600/158

View Patent ↗

Loading inventors, assignments & file history…

Monitor This Case

Get email alerts when status or documents change.

Order Certified Copies

Most orders are placed with the USPTO same day — all within 24 business hours.

Order via The Patent Place →

Pre-filled with this patent's details

Quick Facts

Patent No.: US 11,555,202
App. No.: 16/471,776
Granted: Jan 17, 2023
Kind: B2

External Links

USPTO Patent Center↗ Full Patent PDF↗

Abstract

The invention provides specific transgenic soybean plants, plant material and seeds, characterized in that these products harbor a specific nematode resistance and herbicide tolerance transformation event at a specific location in the soybean genome. Tools are also provided which allow rapid and unequivocal identification of the event in biological samples.

Claims (32)

1. A nucleic acid molecule comprising

(a) a nucleotide sequence with at least 99% sequence identity to the nucleotide sequence of SEQ ID No. 5, 6, 24 or 25, or to the complement of SEQ ID No. 5, 6, 24 or 25;

(b) the nucleotide sequence of SEQ ID No. 1, 2, 3, 4, 5, 6, 24, or 25 or the complement of SEQ ID No. 1, 2, 3, 4, 5, 6, 24, or 25; or

(c) the nucleotide sequence of SEQ ID No. 1 or 3 and the nucleotide sequence of SEQ ID No. 2 or 4, or the complement of SEQ ID No. 1 or 3 and the complement of SEQ ID No. 2 or 4.

2. The nucleic acid molecule of claim 1 , further comprising:

(a) the nucleotide sequence of SEQ ID No. 7 and 9, or the complement of SEQ ID No. 7 and 9;

(b) the nucleotide sequence of SEQ ID No. 11 from nucleotide position 188 to nucleotide position 7368; or

(c) a nucleotide sequence having at least 98%, or least 99%, or at least 99.5% or at least 99.9% sequence identity to the nucleotide sequence of (a) or (b).

3. The nucleic acid molecule of claim 2 , wherein the molecule comprises the nucleotide sequence of SEQ ID No. 5 or 24 and SEQ ID No. 6 or 25, or the complement of SEQ ID No. 5 or 24 and SEQ ID No. 6 or 25.

4. A nucleic acid molecule obtainable from the seed deposited at the ATCC under accession number PTA-123625, wherein said nucleic acid molecule comprises the nucleotide sequence of any one of SEQ ID No. 1, 3, or 5 and the nucleotide sequence of any one of SEQ ID No. 2, 4, or 6.

5. Soybean genomic DNA comprising the nucleic acid molecule of claim 1 .

6. A nucleic acid molecule comprising the nucleotide sequence of SEQ ID No. 11 from nucleotide position 131 to nucleotide position 7941, or a nucleotide sequence having at least 95%, at least 96%, at least 97%, at least 98%, or at least 99% sequence identity to the nucleotide sequence of SEQ ID No. 11 from nucleotide position 131 to nucleotide position 7941.

7. A nucleic acid molecule that specifically characterizes soybean elite transformation event EE-GM5, wherein it comprises (i) the nucleotide sequence of any one of SEQ ID No. 1, 3, or 5, and/or (ii) the nucleotide sequence of SEQ ID No. 2, 4, or 6.

8. A nucleic acid molecule comprising (a) a nucleotide sequence with at least 98% sequence identity to SEQ ID No. 7 or 9; or (b) a nucleotide sequence with at least 95% sequence identity to SEQ ID No. 11.

9. The nucleic acid molecule of claim 1 , wherein the nucleic acid molecule comprises a nucleotide sequence with at least 99% sequence identity to the nucleotide sequence of SEQ ID No. 5, 6, 24 or 25, or to the complement of SEQ ID No. 5, 6, 24 or 25.

10. The nucleic acid molecule of claim 1 , wherein the nucleic acid molecule comprises the nucleotide sequence of SEQ ID No. 1 or the complement of SEQ ID No. 1.

11. The nucleic acid molecule of claim 1 , wherein the nucleic acid molecule comprises the nucleotide sequence of SEQ ID No. 2 or the complement of SEQ ID No. 2.

12. The nucleic acid molecule of claim 1 , wherein the nucleic acid molecule comprises the nucleotide sequence of SEQ ID No. 3 or the complement of SEQ ID No. 3.

13. The nucleic acid molecule of claim 1 , wherein the nucleic acid molecule comprises the nucleotide sequence of SEQ ID No. 4 or the complement of SEQ ID No. 4.

14. The nucleic acid molecule of claim 1 , wherein the nucleic acid molecule comprises the nucleotide sequence of SEQ ID No. 5 or the complement of SEQ ID No. 5.

15. The nucleic acid molecule of claim 1 , wherein the nucleic acid molecule comprises the nucleotide sequence of SEQ ID No. 6 or the complement of SEQ ID No. 6.

16. The nucleic acid molecule of claim 1 , wherein the nucleic acid molecule comprises the nucleotide sequence of SEQ ID No. 24 or the complement of SEQ ID No. 24.

17. The nucleic acid molecule of claim 1 , wherein the nucleic acid molecule comprises the nucleotide sequence of SEQ ID No. 25 or the complement of SEQ ID No. 25.

18. The nucleic acid molecule of claim 1 , wherein the nucleic acid molecule comprises the nucleotide sequence of SEQ ID No. 1 or 3 and the nucleotide sequence of SEQ ID No. 2 or 4, or the complement of SEQ ID No. 1 or 3 and the complement of SEQ ID No. 2 or 4.

19. The nucleic acid molecule of claim 6 , wherein the nucleic acid molecule comprises the nucleotide sequence of SEQ ID No. 11 from nucleotide position 131 to nucleotide position 7941.

20. The nucleic acid molecule of claim 6 , wherein the nucleic acid molecule comprises a nucleotide sequence having at least 95% sequence identity to the nucleotide sequence of SEQ ID No. 11 from nucleotide position 131 to nucleotide position 7941.

21. The nucleic acid molecule of claim 6 , wherein the nucleic acid molecule comprises a nucleotide sequence having at least 96% sequence identity to the nucleotide sequence of SEQ ID No. 11 from nucleotide position 131 to nucleotide position 7941.

22. The nucleic acid molecule of claim 6 , wherein the nucleic acid molecule comprises a nucleotide sequence having at least 97% sequence identity to the nucleotide sequence of SEQ ID No. 11 from nucleotide position 131 to nucleotide position 7941.

23. The nucleic acid molecule of claim 6 , wherein the nucleic acid molecule comprises a nucleotide sequence having at least 98% sequence identity to the nucleotide sequence of SEQ ID No. 11 from nucleotide position 131 to nucleotide position 7941.

24. The nucleic acid molecule of claim 6 , wherein the nucleic acid molecule comprises a nucleotide sequence having at least 99% sequence identity to the nucleotide sequence of SEQ ID No. 11 from nucleotide position 131 to nucleotide position 7941.

25. The nucleic acid molecule of claim 8 , wherein the nucleic acid molecule comprises a nucleotide sequence with at least 98% sequence identity to SEQ ID No. 7 or 9.

26. The nucleic acid molecule of claim 8 , wherein the nucleic acid molecule comprises a nucleotide sequence with at least 95% sequence identity to SEQ ID No. 11.

Assignments (6)

CHANGE OF NAME Recorded Jan 20, 2025

From: BASF AGRICULTURAL SOLUTIONS SEED US LLC

To: BASF AGRICULTURAL SOLUTIONS US LLC

Reel/Frame 069957/0265 →

ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Sep 16, 2021

From: BASF SE

To: BASF AGRICULTURAL SOLUTIONS SEED, US LLC

Reel/Frame 057496/0559 →

ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Sep 26, 2019

From: MOSER, HAL S; MCCARVILLE, MICHAEL; BEILINSON, VADIM; KLEVEN, THOMAS W; DAUM, JULIA

To: BAYER CROPSCIENCE LP