Mixing system
The present invention relates to a mixing system for a bioreactor, comprising a plurality of supply units ( 10 ), each being able to hold media for use in a bioreactor a mixing unit ( 30 ) for creating a uniform mixing of media a first feeding mechanism ( 20 ), arranged to feed media from the supply units ( 10 ) into the mixing unit ( 30 ), a control unit ( 40 ) operatively connected to the first feeding mechanism ( 20 ) and the mixing unit ( 30 ), said control unit ( 40 ) being configured to control the first feeding mechanism ( 20 ) to feed predetermined amounts of media from the plurality of supply units ( 10 ) to the mixing unit ( 30 ), and further being configured to control the mixing unit ( 30 ) to create a uniform mixing of media. The invention also relates to a method for mixing media for use in a bioreactor.
1. A mixing system for a bioreactor, for producing a mixture prior to it being fed into the bioreactor, the mixing system comprising: a cell bag; a plurality of supply units, each being able to hold media for use in a bioreactor; a mixing unit for creating a uniform mixing of media; a first feeding mechanism, arranged to feed media from the supply units into the mixing unit; a second feeding mechanism arranged to feed media from the mixing unit to the cell bag; a refrigeration system configured to maintain contents of a first supply unit of the plurality of supply units at a first temperature, and of a second supply unit of the plurality of supply units at a second temperature; and a control unit operatively connected to the first feeding mechanism and the mixing unit, said control unit being configured to control the first feeding mechanism to feed predetermined amounts of media from the plurality of supply units to the mixing unit, further being configured to control the mixing unit to create a uniform mixing of media, and further being configured to control the mixing system to control a temperature of the mixture.
2. The mixing system according to claim 1 , wherein the mixing system is integrated with a bioreactor.
3. The mixing system according to claim 1 , wherein the first feeding mechanism comprises pumps for pumping media from each of the supply units to the mixing unit and wherein the pumps are peristaltic pumps.
4. The mixing system according to claim 1 , wherein the first feeding mechanism comprises valves for controlling a flow of media from each of the supply units to the mixing unit.
5. The mixing system according to claim 1 , wherein the bioreactor is operatively connected to and able to control the operation of the mixing unit.
6. The mixing system according to claim 3 , wherein the peristaltic pumps are controlled by at least one stepper motor.
7. The mixing system according to claim 1 , further comprising a pH sensor operatively connected to the control unit and arranged to measure a pH value in the mixing unit, and wherein the control unit further comprises an input for receiving pH data from a bioreactor.
8. The mixing system according to claim 7 , wherein the control unit is able to receive pH data from the pH sensor and from the input, and wherein the control unit is arranged to control the operation of the first feeding mechanism in response to the pH data.
9. The mixing system of claim 8 , wherein the control unit is further configured to control the mixing system to control a pH of the mixture.
10. The mixing system according to claim 1 , wherein the refrigeration system is further arranged to maintain contents of at least another of the supply units at another predetermined temperature that is different from the first or second predetermined temperatures.
11. The mixing system according to claim 1 , further comprising a temperature sensor operatively connected to the control unit and arranged to measure a temperature in the mixing unit, wherein the control unit is also arranged to control the first feeding mechanism in response to input from the temperature sensor.
12. The mixing system according to claim 1 , further comprising a heating mechanism arranged to heat media in the mixing unit or in at least one of the supply units.
13. The mixing system according to claim 1 , wherein the control unit is arranged to control the mixing system in response to a predetermined program.
14. The mixing system according to claim 1 , wherein the control unit is arranged to control the mixing system in response to input from a user.