Gas-fed fermentation reactors, systems and processes utilizing a vertical flow zone
Reactors, systems and processes for the production of biomass by culturing microorganisms in aqueous liquid culture medium circulating in a loop reactor which utilize substantially vertical flow zones are described. Recovery and processing of the culture microorganisms to obtain products, such as proteins or hydrocarbons is described.
1. A system for stimulating production of biomass comprising:
a loop reactor, the loop reactor including:
a gas/liquid separation vessel for separating a multi-phase mixture of a gas and a liquid culture medium into a gas phase and a liquid phase, the gas/liquid separation vessel including an outlet and an inlet;
a loop section including:
an inlet in fluid communication with the outlet of the gas/liquid separation vessel and an outlet in fluid communication with the inlet of the gas/liquid separation vessel;
a first substantially horizontal zone between the loop section inlet and the loop section outlet, the first substantially horizontal zone occupying a first substantially horizontal plane and including a first flow section, a second flow section and a linear fifth flow section between the first flow section and the second flow section, where, when the loop reactor is in operation, the multi-phase mixture flows in a first direction in the first flow section, flows in a second direction in the second flow section and flows in a fifth direction in the linear fifth flow section, the first direction, second direction and fifth direction being different from each other;
a second substantially horizontal zone between the loop section inlet and the loop section outlet, the second substantially horizontal zone occupying a second substantially horizontal plane different from the first substantially horizontal plane and including a third flow section, fourth flow section and a linear sixth flow section between the third flow section and the fourth flow section, where, when the loop reactor is in operation, the multi-phase mixture flows in a third direction in the third flow section, flows in a fourth direction in the fourth flow section and flows in a sixth direction in the linear sixth flow section, the third direction, fourth direction and sixth direction being different from each other, at least a portion of the second substantially horizontal zone overlapping at least a portion of the first substantially horizontal zone; and
a first substantially vertical flow zone, the first substantially vertical flow zone located between the inlet of the loop section and the outlet of the loop section.
2. The system of claim 1 wherein mass transfer between the gas and the liquid culture medium of the multi-phase mixture in the first substantially horizontal zone, when the loop reactor is in operation, is characterized by a substantially first horizontal zone volumetric mass transfer rate (kLa), mass transfer between the gas and the liquid culture medium of the multi-phase mixture in the first substantially vertical flow zone, when the loop reactor is in operation, is characterized by a first substantially vertical flow zone volumetric mass transfer rate (kLa), and mass transfer between the gas and the liquid culture medium of the multi-phase mixture in the second substantially horizontal flow zone, when the loop reactor is in operation, characterized by a second substantially horizontal flow zone volumetric mass transfer rate (kLa), wherein the first substantially vertical flow zone volumetric mass transfer rate (kLa) is 2 to 5 times greater than at least one of the first substantially horizontal zone volumetric mass transfer rate (kLa) or the second substantially horizontal zone volumetric mass transfer rate (kLa).
3. The system of claim 1 , wherein the second substantially horizontal zone crosses over the first substantially horizontal zone.
4. The system of claim 1 , wherein when the loop reactor is in operation, the first direction of flow of the multi-phase mixture in the first flow section is the same as the third direction of flow of the multi-phase mixture in the third flow section.
5. The system of claim 1 , wherein when the loop reactor is in operation, the fifth direction of flow of the multi-phase mixture in the linear fifth flow section is opposite to the sixth direction of flow in the linear sixth flow section.
6. The system of claim 1 , wherein the first flow section is in fluid communication with the linear fifth flow section via a 90° elbow.
7. The system of claim 1 , wherein the linear fifth flow section is in fluid communication with the second flow section via a 90° elbow.
8. The system of claim 1 , wherein the first substantially vertical flow zone is in fluid communication with the second flow section via a 90° elbow.
9. The system of claim 1 , wherein the first substantially vertical flow zone is in fluid communication with the third flow section via a 90° elbow.
10. The system of claim 1 , wherein the third flow section is in fluid communication with the linear sixth flow section via a 90° elbow.
11. The system of claim 1 , wherein the linear sixth flow section is in fluid communication with the fourth flow section via a 90° elbow.
12. A process for stimulating production of biomass in a loop reactor comprising:
passing the multi-phase mixture of gas and a liquid culture medium through a first substantially horizontal zone of the loop reactor, the first substantially horizontal zone occupying a first substantially horizontal plane and including a first flow section, a second flow section and a linear fifth flow section between the first flow section and the second flow section, the passing the multiphase mixture of gas and a liquid culture medium through the first substantially horizontal zone including flowing the multi-phase mixture in a first direction through the first flow section, flowing the multiphase mixture through the second flow section in a second direction and flowing the multiphase mixture through the linear fifth flow section in a fifth direction, the first direction, second direction and fifth direction being different from each other;
in the first substantially horizontal zone, transferring the gas of the multi-phase mixture to the liquid culture medium of the multi-phase mixture;
passing the multi-phase mixture of a gas and a liquid culture medium from the first substantially horizontal zone through a first substantially vertical flow zone of the loop reactor;
in the first substantially vertical flow zone, transferring the gas of the multi-phase mixture to the liquid culture medium of the multi-phase mixture;
passing the multi-phase mixture of gas and a liquid culture medium from the first substantially vertical flow zone into a second substantially horizontal zone of the loop reactor, the second substantially horizontal zone occupying a second substantially horizontal plane different from the first substantially horizontal plane and including a third flow section, a fourth flow section and a linear sixth flow section, the passing the multiphase mixture of gas and a liquid culture medium through the second substantially horizontal zone including flowing the multi-phase mixture in a third direction through the third flow section, flowing the multiphase mixture through the fourth flow section in a fourth direction and flowing the multiphase mixture through the linear sixth flow section in a sixth direction, the third direction, fourth direction and sixth direction being different from each other, at least a portion of the second substantially horizontal zone overlapping at least a portion of the first substantially horizontal zone;
separating the multi-phase mixture of a gas and a liquid culture medium into a gas phase and a liquid phase downstream of the second substantially horizontal flow zone;
passing the gas phase and the liquid phase separated from the multi-phase mixture of a gas and a liquid culture medium into a gas/liquid separation vessel through an inlet to the gas/liquid separation vessel; and
removing a liquid phase from an outlet of the gas/liquid separation vessel and delivering the removed liquid phase to an inlet of a loop section of the loop reactor.
13. The process of claim 12 , wherein transferring the gas of the multi-phase mixture to the liquid culture medium of the multi-phase mixture in the first substantially vertical flow zone occurs at a first substantially vertical flow zone volumetric mass transfer rate (kLa), transferring the gas of the multi-phase mixture to the liquid culture medium of the multi-phase mixture in the first substantially horizontal zone occurs at a first substantially horizontal zone volumetric mass transfer rate (kLa), and transferring the gas of the multi-phase mixture to the liquid culture medium of the multi-phase mixture in the second substantially horizontal flow zone occurs at a second substantially horizontal flow zone volumetric mass transfer rate (kLa), wherein the first substantially vertical flow zone volumetric mass transfer rate (kLa) is 2-5 times greater than at least one of the first substantially horizontal zone volumetric mass transfer rate (kLa) or the second substantially horizontal zone volumetric mass transfer rate (kLa).