Use of BP005 for the control of plant pathogens
Compositions and methods for conferring resistance to a plant pathogen are provided. Compositions comprising a coding sequence for a polypeptide having antifungal activity are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in any of SEQ ID NO:1-67, or the nucleotide sequence set forth in any of SEQ ID NO:69-81, 83-95, or 97-106, as well as variants and fragments thereof.
1. A method of conferring disease resistance in a plant, the method comprising transforming said plant with a nucleotide sequence, wherein the nucleotide sequence comprises:
a) the nucleotide sequence set forth in any of SEQ ID NO: 69-76, 78, 80, 81, 83-88, 90-92, 99-101, 104-106;
b) a nucleotide sequence that encodes a polypeptide comprising the amino acid sequence of any of SEQ ID NO: 1-9, 11, 13-14, 16-21, 23-25, 32-34, 37-42, 44-58 and 60-67; and
c) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 85% sequence identity to the amino acid sequence of any of SEQ ID NO: 5 or 7;
d) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 90% sequence identity to the amino acid sequence of any of SEQ ID NO: 6, 8, 17, or 24;
e) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 95% sequence identity to the amino acid sequence of any of SEQ ID NO: 9, 11, 13, 14, 16, 18-20, 23, 25, 32-34, 37, 38 or 53; or
f) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 98% sequence identity to the amino acid sequence of any of SEQ ID NO: 21, 54-57, or 63;
wherein the disease is caused by a fungal pathogen.
2. The method of claim 1 , wherein the fungal pathogen is Phakopsora pachyrhizi or Phakopsora meibomiae.
3. The method of claim 1 , wherein said plant is selected from the group consisting of maize, sorghum, wheat, cabbage, sunflower, tomato, crucifers, peppers, potato, cotton, rice, soybean, sugarbeet, sugarcane, tobacco, barley, and oilseed rape.
4. The method of claim 1 , wherein the nucleotide sequence encodes a polypeptide comprising an amino acid sequence having at least 90% sequence identity to the amino acid sequence of any of SEQ ID NO: 5-8, 17, or 24.
5. The method of claim 1 , wherein the nucleotide sequence encodes a polypeptide comprising an amino acid sequence having at least 95% sequence identity to the amino acid sequence of any of SEQ ID NO: 5-9, 11, 13, 14, 16-20, 23-25, 32-34, 37, 38 or 53.
6. The method of claim 1 , wherein the nucleotide sequence encodes a polypeptide comprising an amino acid sequence having at least 99% sequence identity to the amino acid sequence of any of SEQ ID NO: 5-9, 11, 13, 14, 16-21, 23-25, 32-34, 37, 38, 53-57 or 63.
7. The method of claim 1 , wherein the nucleotide sequence encodes a polypeptide comprising the amino acid sequence of any of SEQ ID NO: 1-9, 11, 13-14, 16-21, 23-25, 32-34, 37-42, 44-58 and 60-67.
8. The method of claim 1 , wherein the nucleotide sequence encodes a polypeptide comprising the amino acid sequence of SEQ ID NO: 24.
9. A method for increasing yield in a plant, the method comprising growing in a field a plant of or a seed thereof having stably incorporated into its genome a DNA construct comprising a nucleotide sequence that encodes a protein having antifungal activity, wherein said nucleotide sequence is:
a) the nucleotide sequence set forth in any of SEQ ID NO: 69-76, 78, 80, 81, 83-88, 90-92, 99-101, 104-106;
b) a nucleotide sequence that encodes a polypeptide comprising the amino acid sequence of any of SEQ ID NO: 1-9, 11, 13-14, 16-21, 23-25, 32-34, 37-42, 44-58 and 60-67; and
c) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 85% sequence identity to the amino acid sequence of any of SEQ ID NO: 5 or 7;
d) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 90% sequence identity to the amino acid sequence of any of SEQ ID NO: 6, 8, 17, or 24;
e) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 95% sequence identity to the amino acid sequence of any of SEQ ID NO: 9, 11, 13, 14, 16, 18-20, 23, 25, 32-34, 37, 38 or 53; or
f) a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 98% sequence identity to the amino acid sequence of any of SEQ ID NO: 21, 54-57, or 63;
wherein said field is infested with a fungal plant pest against which said polypeptide has antifungal activity.
10. The method of claim 9 , wherein the nucleotide sequence encodes a polypeptide comprising an amino acid sequence having at least 90% sequence identity to the amino acid sequence of any of SEQ ID NO: 5-8, 17, or 24.
11. The method of claim 9 , wherein the nucleotide sequence encodes a polypeptide comprising an amino acid sequence having at least 95% sequence identity to the amino acid sequence of any of SEQ ID NO: 5-9, 11, 13, 14, 16-20, 23-25, 32-34, 37, 38 or 53.
12. The method of claim 9 , wherein the nucleotide sequence encodes a polypeptide comprising an amino acid sequence having at least 99% sequence identity to the amino acid sequence of any of SEQ ID NO: 5-9, 11, 13, 14, 16-21, 23-25, 32-34, 37, 38, 53-57 or 63.
13. The method of claim 9 , wherein the nucleotide sequence encodes a polypeptide comprising the amino acid sequence of any of SEQ ID NO: 1-9, 11, 13-14, 16-21, 23-25, 32-34, 37-42, 44-58 and 60-67.
14. The method of claim 9 , wherein the nucleotide sequence encodes a polypeptide comprising the amino acid sequence of SEQ ID NO: 24.
15. The method of claim 9 , wherein the fungal plant pest is Phakopsora pachyrhizi or Phakopsora meibomiae.
16. The method of claim 9 , wherein said plant is selected from the group consisting of maize, sorghum, wheat, cabbage, sunflower, tomato, crucifers, peppers, potato, cotton, rice, soybean, sugarbeet, sugarcane, tobacco, barley, and oilseed rape.