Substituted quinazoline and quinazolinone compounds and methods of use thereof
Compounds having activity as inhibitors of G12C mutant KRAS protein are provided. The compounds have the following structure (I): (Formula (I)) or a pharmaceutically acceptable salt, stereoisomer thereof, wherein G, Y, R, R 1 , R 2a , R 2b , R 2c , L, L 1 and E are as defined herein. Methods associated with preparation and use of such compounds, pharmaceutical compositions comprising such compounds and methods to modulate the activity of G12C mutant KRAS protein for treatment of disorders, such as cancer, are also provided. Preferred compounds are quinazoline and quinazolinone derivatives and in particular 1-(6-(3-hydroxynaphthalen-1-yl)quinazolin-2-yl)azetidin-1-yl)prop-2-en-1-one derivatives and similar compounds.
1. A compound having the following structure (I):
or a pharmaceutically acceptable salt, stereoisomer or prodrug thereof, wherein:
G is —C(═O)— or —C(R 3 )═;
Y is N;
R is absent when G is —C(R 3 )═; or R is H, aminocarbonyl, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, heterocyclylalkyl, aryl, heteroaryl, arylalkyl, heteroarylalkyl, C 2 -C 6 alkynyl, C 2 -C 6 alkenyl, C 1 -C 6 alkylaminyl, aminylalkyl, C 3 -C 8 cycloalkyl, or C 3 -C 8 heterocyclyl when G is —C(═O)—;
R 1 is cycloalkyl, heterocyclyl, aryl or heteroaryl;
R 2a , R 2b and R 2c are, at each occurrence, independently H, amino, cyano, halo, hydroxyl, C 1 -C 6 alkyl, C 1 -C 6 alkylamino, C 1 -C 6 haloalkyl, C 1 -C 6 alkoxy, C 1 -C 6 haloalkoxy, C 3 -C 8 cycloalkyl, heterocyclylalkyl, C 2 -C 6 alkynyl, C 2 -C 6 alkenyl, aminylalkyl, alkylaminylalkyl, cyanoalkyl, carboxyalkyl, aminylcarbonylalkyl, aminylcarbonyl, heteroaryl or aryl;
R 3 is H, cyano, CF 3 , amide, sulfonamide, cyanoalkyl, amino, aminylalkyl, aminylalkylaminyl, aminylcarbonyl, alkylaminyl, haloalkylaminyl, hydroxylalkyaminyl, amindinylalkyl, amidinylalkoxy, amindinylalkylaminyl, guanidinylalkyl, guanidinylalkoxy, guanidinylalkylaminyl, C 1 -C 6 alkoxy, aminylalkoxy, alkylaminylalkoxy, alkylcarbonylaminylalkoxy, C 1 -C 6 alkyl, heterocyclyl, heterocyclyloxy, heterocyclylalkyloxy, heterocyclylaminyl, heterocyclylalkylaminyl, heteroaryl, heteroaryloxy, heteroarylalkyloxy, heteroarylaminyl, heteroarylalkylaminyl, aryl, aryloxy, arylaminyl, arylalkylaminyl or arylalkyloxy;
L is, at each occurrence, independently absent or a linker selected from the group consisting of —O—, —NR a —, —NR a C(═O)—, —NR a S(═O) 2 — and —S(═O) 2 —, wherein R a is H or C 1 -C 6 alkyl;
L 1 is alkylene, heteroalkylene, heterocyclylene, aminylheterocyclylene, alkylheterocyclylene, heterocyclylenealkyl or heteroalkylheterocyclylene, wherein when R 3 is H and L 1 is heterocyclylene, the heterocyclylene is monocyclic; and
E is an electrophilic moiety capable of forming a covalent bond with the cysteine residue at position 12 of a KRAS, HRAS or NRAS G12C mutant protein.
2. The compound of claim 1 , having the following structure (Ia):
wherein:
Y is N;
R is H, aminocarbonyl, C 1 -C 6 alkyl, C 2 -C 6 alkynyl, C 2 -C 6 alkenyl, C 1 -C 6 alkylaminyl, C 1 -C 6 haloalkyl, C 3 -C 8 cycloalkyl, or C 3 -C 8 heterocyclyl;
R 1 is cycloalkyl, heterocyclyl, aryl or heteroaryl;
R 2a , R 2b and R 2c are, at each occurrence, independently H, amino, cyano, halo, hydroxyl, C 1 -C 6 alkyl, C 1 -C 6 alkylamino, C 1 -C 6 haloalkyl, C 1 -C 6 alkoxy, C 1 -C 6 haloalkoxy, C 3 -C 8 cycloalkyl, heterocyclylalkyl, C 2 -C 6 alkynyl, C 2 -C 6 alkenyl, aminylalkyl, alkylaminylalkyl, cyanoalkyl, carboxyalkyl, aminylcarbonylalkyl, aminylcarbonyl, heteroaryl or aryl;
L is, at each occurrence, independently absent or a linker selected from the group consisting of —O—, —NR a —, —NR a C(═O)—, —NR a S(═O) 2 — and —S(═O) 2 —, wherein R a is H or C 1 -C 6 alkyl;
L 1 is alkylene, heteroalkylene, heterocyclylene, aminylheterocyclylene, alkylheterocyclylene or heteroalkylheterocyclylene; and
E is an electrophilic moiety capable of forming a covalent bond with the cysteine residue at position 12 of a KRAS, HRAS or NRAS G12C mutant protein.
3. The compound of claim 1 , having the following structure (Ib):
wherein:
Y is N;
R 1 is cycloalkyl, heterocyclyl, aryl or heteroaryl;
R 2a , R 2b and R 2c are, at each occurrence, independently H, amino, cyano, halo, hydroxyl, C 1 -C 6 alkyl, C 1 -C 6 alkylamino, C 1 -C 6 haloalkyl, C 1 -C 6 alkoxy, C 1 -C 6 haloalkoxy; C 3 -C 8 cycloalkyl, heterocyclylalkyl, C 2 -C 6 alkynyl, C 2 -C 6 alkenyl, aminylalkyl, alkylaminylalkyl, cyanoalkyl, carboxyalkyl, aminylcarbonylalkyl, aminylcarbonyl, heteroaryl or aryl;
R 3 is H, cyano, CF 3 , amide, sulfonamide, cyanoalkyl, amino, aminylalkyl, aminylalkylaminyl, aminylcarbonyl, alkylaminyl, haloalkylaminyl, hydroxylalkyaminyl, amindinylalkyl, amidinylalkoxy, amindinylalkylaminyl, guanidinylalkyl, guanidinylalkoxy, guanidinylalkylaminyl, C 1 -C 6 alkoxy, aminylalkoxy, alkylaminylalkoxy, alkylcarbonylaminylalkoxy, C 1 -C 6 alkyl, heterocyclyl, heterocyclyloxy, heterocyclylalkyloxy, heterocyclylaminyl, heterocyclylalkylaminyl, heteroaryl, heteroaryloxy, heteroarylalkyloxy, heteroarylaminyl, heteroarylalkylaminyl, aryl, aryloxy, arylaminyl, arylalkylaminyl or arylalkyloxy;
L is, at each occurrence, independently absent or a linker selected from the group consisting of —O—, —NR a —, —NR a C(═O)—, —NR a S(═O) 2 — and —S(═O) 2 —, wherein R a is H or C 1 -C 6 alkyl;
L 1 is alkylene, heteroalkylene, heterocyclylene, aminylheterocyclylene, alkylheterocyclylene or heteroalkylheterocyclylene; and
E is an electrophilic moiety capable of forming a covalent bond with the cysteine residue at position 12 of a KRAS, HRAS or NRAS G12C mutant protein.
4. The compound of claim 1 , having the following structure (Ic):
wherein:
X 1 and X 2 are independently N or CH, provided at least one of X 1 and X 2 is N;
R 4a and R 4b are, at each occurrence, independently H, —OH, —NH 2 , —CO 2 H, halo, cyano, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 haloalkoxy, C 2 -C 6 alkynyl, hydroxylalkyl, alkoxyalkyl, aminylalkyl, alkylaminylalkyl, cyanoalkyl, carboxyalkyl, aminylcarbonylalkyl or aminylcarbonyl; or R 4a and R 4b join to form oxo, a carbocyclic ring or a heterocyclic ring; or R 4a is H, —OH, —NH 2 , —CO 2 H, halo, cyano, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 haloalkoxy, C 2 -C 6 alkynyl, hydroxylalkyl, alkoxyalkyl, aminylalkyl, alkylaminylalkyl, cyanoalkyl, carboxyalkyl, aminylcarbonylalkyl or aminylcarbonyl, and R 4b joins with R 5b to form a carbocyclic or heterocyclic ring;
R 5a and R 5b are, at each occurrence, independently H, —OH, —NH 2 , —CO 2 H, halo, cyano, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 haloalkoxy, C 2 -C 6 alkynyl, hydroxylalkyl, alkoxyalkyl, aminylalkyl, alkylaminylalkyl, cyanoalkyl, carboxyalkyl, aminylcarbonylalkyl or aminylcarbonyl; or R 5′ and R 5b join to form oxo, a carbocyclic ring or a heterocyclic ring; or R 5a is H, —OH, —NH 2 , —CO 2 H, halo, cyano, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 haloalkoxy, C 2 -C 6 alkynyl, hydroxylalkyl, alkoxyalkyl, aminylalkyl, alkylaminylalkyl, cyanoalkyl, carboxyalkyl, aminylcarbonylalkyl or aminylcarbonyl, and R 5b joins with R 4b to form a carbocyclic or heterocyclic ring; and
m 1 and m 2 are each independently 1, 2 or 3.
5. The compound of claim 4 , the following structure (Id):
wherein:
represents a double or triple bond;
L 2 is a bond or alkylene;
Z is —C(═O)—, —C(═NR 8 )—, —NR 9 C(═O)—, —S(═O) 2 — or —NR 9 S(═O) 2 —;
when is a double bond then R 6 and R 7 are each independently H, halo, cyano, carboxyl, C 1 -C 6 alkyl, alkoxycarbonyl, aminylalkyl, alkylaminylalkyl, aryl, heterocyclyl, heterocyclylalkyl, heteroaryl or hydroxylalkyl, or R 6 and R 7 join to form a carbocyclic, heterocyclic or heteroaryl ring;
when is a triple bond then R 6 is absent and R 7 is H, C 1 -C 6 alkyl, aminylalkyl, alkylaminylalkyl or hydroxylalkyl;
R 8 is H, —OH, —CN or C 1 -C 6 alkyl; and
R 9 is H, C 1 -C 6 alkyl, hydroxylalkyl, aminoalkyl, alkoxyalkyl, aminylalkyl, alkylaminylalkyl, cyanoalkyl, carboxyalkyl, aminylcarbonylalkyl, C 3 -C 8 cycloalkyl or heterocyclylalkyl.
6. The compound of claim 1 , having the following structure (Ie):
wherein:
X 1 and X 2 are independently N or CH, provided at least one of X 1 and X 2 is N;
R 4a and R 4b are, at each occurrence, independently H, —OH, —NH 2 , —CO 2 H, halo, cyano, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 haloalkoxy, C 2 -C 6 alkynyl, hydroxylalkyl, alkoxyalkyl, aminylalkyl, alkylaminylalkyl, cyanoalkyl, carboxyalkyl, aminylcarbonylalkyl or aminylcarbonyl; or R 4a and R 4b join to form oxo, a carbocyclic ring or a heterocyclic ring; or R 4a is H, —OH, —NH 2 , —CO 2 H, halo, cyano, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 haloalkoxy, C 2 -C 6 alkynyl, hydroxylalkyl, alkoxyalkyl, aminylalkyl, alkylaminylalkyl, cyanoalkyl, carboxyalkyl, aminylcarbonylalkyl or aminylcarbonyl, and R 4b joins with R 5b to form a carbocyclic or heterocyclic ring;
R 5a and R 5b are, at each occurrence, independently H, —OH, —NH 2 , —CO 2 H, halo, cyano, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 haloalkoxy, C 2 -C 6 alkynyl, hydroxylalkyl, alkoxyalkyl, aminylalkyl, alkylaminylalkyl, cyanoalkyl, carboxyalkyl, aminylcarbonylalkyl or aminylcarbonyl; or R 5a and R 5b join to form oxo, a carbocyclic ring or a heterocyclic ring; or R 5a is H, —OH, —NH 2 , —CO 2 H, halo, cyano, C 1 -C 6 alkyl, C 1 -C 6 haloalkyl, C 1 -C 6 haloalkoxy, C 2 -C 6 alkynyl, hydroxylalkyl, alkoxyalkyl, aminylalkyl, alkylaminylalkyl, cyanoalkyl, carboxyalkyl, aminylcarbonylalkyl or aminylcarbonyl, and R 5b joins with R 4b to form a carbocyclic or heterocyclic ring; and
m 1 and m 2 are each independently 1, 2 or 3.
7. The compound of claim 6 , having the structure (If):
wherein:
represents a double or triple bond;
L 2 is a bond or alkylene;
Z is —C(═O)—, —C(═NR 8 )—, —NR 9 C(═O)—, —S(═O) 2 — or —NR 9 S(═O) 2 —;
when is a double bond then R 6 and R 7 are each independently H, halo, cyano, carboxyl, C 1 -C 6 alkyl, alkoxycarbonyl, aminylalkyl, alkylaminylalkyl, aryl, heterocyclyl, heterocyclylalkyl, heteroaryl or hydroxylalkyl, or R 6 and R 7 join to form a carbocyclic, heterocyclic or heteroaryl ring;
when is a triple bond then R 6 is absent and R 7 is H, C 1 -C 6 alkyl, aminylalkyl, alkylaminylalkyl or hydroxylalkyl;
R 8 is H, —OH, —CN or C 1 -C 6 alkyl; and
R 9 is H, C 1 -C 6 alkyl, hydroxylalkyl, aminoalkyl, alkoxyalkyl, aminylalkyl, alkylaminylalkyl, cyanoalkyl, carboxyalkyl, aminylcarbonylalkyl, C 3 -C 8 cycloalkyl or heterocyclylalkyl.
8. The compound of claim 4 , wherein X 2 is N, or wherein X 1 is CH.
9. The compound of claim 5 , the structure (Ig):
wherein:
R 2a , R 2b and R 2c are independently H, amino, cyano, halo, hydroxyl, C 1 -C 6 alkyl, C 1 -C 6 alkylamino, C 1 -C 6 haloalkyl, C 1 -C 6 alkoxy, C 1 -C 6 haloalkoxy, C 3 -C 8 cycloalkyl, heterocyclylalkyl, C 2 -C 6 alkynyl, C 2 -C 6 alkenyl, aminylalkyl, alkylaminylalkyl, cyanoalkyl, carboxyalkyl, aminylcarbonylalkyl, aminylcarbonyl, heteroaryl or aryl.
10. The compound of claim 7 , having the structure (Ih):
wherein:
R 2a , R 2b and R 2c are independently H, amino, cyano, halo, hydroxyl, C 1 -C 6 alkyl, C 1 -C 6 alkylamino, C 1 -C 6 haloalkyl, C 1 -C 6 alkoxy, C 1 -C 6 haloalkoxy, C 3 -C 8 cycloalkyl, heterocyclylalkyl, C 2 -C 6 alkynyl, C 2 -C 6 alkenyl, aminylalkyl, alkylaminylalkyl, cyanoalkyl, carboxyalkyl, aminylcarbonylalkyl, aminylcarbonyl, heteroaryl or aryl.
11. The compound of claim 1 , wherein R 1 is substituted with halo, amino, hydroxyl, C 1 -C 6 alkyl, cyano, C 1 -C 6 haloalkyl, C 1 -C 6 alkoxy, alkylaminyl, cycloalkyl, heterocyclylalkyl, aryl, heteroaryl, phosphate, phosphoalkoxy, boronic acid, boronic acid ester, —OC(═O)R 10 or C 1 -C 6 alkylcarbonyloxy, or combinations thereof, wherein R 10 is C 1 -C 6 alkyl.
12. The compound of claim 1 , wherein R 1 has one of the following structures:
13. The compound of claim 1 , wherein R 2a , R 2b , and R 2c are, at each occurrence, independently H, chloro, fluoro, cyano, hydroxyl, methyl, —C(═O)NH 2 or trifluoromethyl.
14. The compound of claim 4 , wherein each R 4a , R 4b , R 5a and R 5b is H.
15. The compound of claim 4 , wherein at least one occurrence of R 4a , R 4b , R 5a or R 5b is C 1 -C 6 alkyl.
16. The compound of claim 5 , wherein Z is —C(═O)—.
17. The compound of claim 5 , wherein each of R 6 and R 7 are H.
18. The compound of claim 1 , wherein E has one of the following structures:
19. The compound of claim 5 , wherein L 2 is a bond.
20. The compound of claim 1 , wherein L is absent.
21. The compound of claim 1 , having one of the following structures:
22. A substantially purified atropisomer according to claim 1 .
23. A pharmaceutical composition comprising a compound of claim 1 and a pharmaceutically acceptable carrier.
24. A method for regulating activity of a KRAS, HRAS or NRAS G12C mutant protein, the method comprising reacting the KRAS G12C mutant protein with the compound of claim 1 .
25. A method for inhibiting proliferation of a cell population, the method comprising contacting the cell population with the compound of claim 1 .
26. A method for preparing a labeled KRAS, HRAS or NRAS G12C mutant protein, the method comprising reacting the KRAS, HRAS or NRAS G12C mutant with a compound of claim 1 , to result in the labeled KRAS, HRAS or NRAS G12C protein.