Proteins and immunizing compositions containing pasteurella proteins and methods of use
The present invention provides isolated proteins isolatable from a Pasteurella spp. such as P. multocida . Also provided by the present invention are compositions that include one or more of the proteins, and methods for making and methods for using the proteins.
1. A composition comprising: an isolated protein having at least 95% similarity to amino acids 25-968 of SEQ ID NO:2, an isolated protein having at least 95% similarity to amino acids 27-790 of SEQ ID NO:4, an isolated protein having at least 80% similarity to amino acids 23-727 of SEQ ID NO:6, an isolated protein having at least 95% similarity to amino acids 25-964 of SEQ ID NO:8, an isolated protein having at least 95% similarity to amino acids 26-848 of SEQ ID NO: 10, an isolated protein having at least 95% similarity to amino acids 27-784 of SEQ ID NO: 12, and an isolated protein having at least 95% similarity to amino acids 25-742 of SEQ ID NO: 14, and an adjuvant.
2. The composition of claim 1 further comprising: at least one isolated protein having a molecular weight of 249 kDa, 60 kDa, 42 kDa, 38 kDa, 27 kDa, 26 kDa, or 22 kDa, wherein the proteins are isolated from a P. multocida.
3. The composition of claim 1 further comprising a pharmaceutically acceptable carrier.
4. The composition of claim 1 further comprising an isolated protein having at least 80% similarity to amino acids 26-805 of SEQ ID NO:44.
5. A method comprising: administering to a subject an amount of the composition of claim 1 effective to induce the subject to produce antibody that specifically binds to at least one protein of the composition.
6. The method of claim 5 wherein the subject is a mammal.
7. The method of claim 6 wherein the mammal is a bovine.
8. The method of claim 5 wherein the subject is an avian.
9. The method of claim 8 wherein the avian is a chicken or a turkey.
10. The method of claim 5 wherein at least 100 micrograms (μg) and no greater than 2,800 μg of protein is administered.
11. A kit for detecting antibody that specifically binds a protein, comprising in one container the isolated proteins of the composition of claim 1 ; and in a separate second container a reagent that detects an antibody that specifically binds to one of the proteins.