Immunotherapeutic treatment method using an interleukin-2 receptor beta-selective agonist in combination with adoptive cell transfer therapy
Provided are methods and compositions directed towards the treatment of an individual having cancer by providing adoptive cell transfer therapy and administering to the individual a long-acting IL-2Rβ-biased agonist.
1. A method for treating a subject with melanoma, the method comprising:
(i) administering to the subject with melanoma, autologous tumor-infiltrating lymphocytes (TILs) that have been harvested from the subject, cultured and expanded ex vivo, and
(ii) administering to the subject an IL-2Rβ-activating amount of a long acting IL-2Rβ-biased agonist of Formula I,
wherein IL-2 is interleukin-2 and each “n” is independently an integer from about 3 to about 4000, or pharmaceutically acceptable salts thereof.
2. The method of claim 1 , wherein step (i) and step (ii) are carried out sequentially, in either order, or substantially simultaneously.
3. The method of claim 1 , wherein step (i) is carried out before step (ii).
4. The method of claim 1 , wherein step (ii) is carried out before step (i).
5. The method of claim 1 , wherein both steps (i) and (ii) are carried out substantially simultaneously.
6. The method of claim 5 , wherein steps (i) and (ii) are both carried out on day 1 of treatment.
7. The method of claim 3 , wherein step (ii) is carried out on any one of days 1 to 7 of treatment.
8. The method of claim 1 , comprising a single administration to the subject of the autologous harvested, cultured and expanded tumor-infiltrating lymphocytes over the course of treatment.
9. The method of claim 1 , comprising multiple administrations to the subject of an IL-2Rβ-activating amount of the long acting IL-2Rβ-biased agonist over the course of treatment.
10. The method of claim 1 , wherein the autologous harvested, cultured and expanded tumor-infiltrating lymphocytes are administered by infusion.
11. The method of claim 1 , wherein the subject is human.
12. The method of claim 1 , wherein the cancer is metastatic melanoma.
13. The method of claim 1 , wherein the IL-2 of Formula (I) is aldesleukin.
14. The method of claim 1 , wherein each “n” is an integer from about 40 to about 550.
15. The method of claim 14 , wherein each “n” is about 227.
16. The method of claim 14 , wherein the long acting IL-2Rβ-biased agonist comprises no more than about 10% (molar amount) of compounds in accordance with Formula II,
wherein IL-2 is interleukin-2, “m” is an integer selected from the group consisting of 1, 2, 3, 7 and >7, or pharmaceutically acceptable salts thereof.
17. The method of claim 14 , wherein the long acting IL-2Rβ-biased agonist in accordance with Formula I possesses on average about six branched polyethylene glycol moieties releasably attached to IL-2.
18. The method of claim 1 , wherein the long acting IL-2Rβ-biased agonist is initially administered within 4 days of administering to the subject the autologous harvested, cultured and expanded tumor-infiltrating lymphocytes.
19. The method of claim 1 , wherein the long acting IL-2Rβ-biased agonist is administered by injection.
20. The method of claim 14 , wherein the method is effective to result in a greater number of melanoma tumor-specific effector T cells retained at a melanoma tumor site of the subject when compared to the number of melanoma tumor-specific effector T-cells retained at a melanoma tumor site of the subject upon treatment of the subject with step (i) alone absent administration of the long-acting IL-2Rβ-biased agonist, or, when compared to the number of tumor-specific effector T-cells retained at a melanoma tumor site of the subject upon treatment of the subject with the harvested, cultured and expanded autologous tumor-infiltrating lymphocytes from step (i) in combination with administration of interleukin-2 dosed to achieve a comparable number of IL-2 equivalents, when measured at a time point that is at least 10 days following the initial administration of the long-acting IL-2Rβ-biased agonist.
21. The method of claim 20 , wherein the time point is at day 10, 11, 12, 13, 14 or 15 following the initial administration of the long-acting IL-2Rβ-biased agonist.
22. The method of claim 1 , wherein the method results in a beneficial response treatment that is enhanced over the response to treatment observed when administration is carried out according to either step (i) or step (ii) alone.
23. The method of claim 21 , wherein the beneficial response to treatment is based on a suitable animal model of melanoma.
24. The method of claim 1 , wherein the method of treatment is effective to inhibit accumulation of regulatory T cells selected from the group consisting of CD4+ Tregs, CD25+ Tregs, and FoxP3+ Tregs in a melanoma tumor of the subject by an amount that is enhanced over that observed upon treatment of the subject with step (i) alone absent administration of the long-acting IL-2Rβ-biased agonist, or, upon treatment of the subject with the autologous harvested, cultured and expanded tumor-infiltrating lymphocytes from step (i) in combination with administration of interleukin-2 dosed to achieve a comparable number of IL-2 equivalents.
25. The method of claim 24 , wherein said inhibition of accumulation of regulatory T cells is evaluated in an in vivo melanoma model.