CRYSTALLINE FORMS OF OBETICHOLIC ACID
The crystalline forms of obeticholic acid and methods of preparation and use thereof are described.
1 . A cocrystalline form of obeticholic acid (OCA) and a co-former.
2 . The cocrystalline form of claim 1 , wherein the co-former is a bile acid or bile acid derivative.
3 . The cocrystalline form of claim 1 , wherein the bile acid is ursodeoxycholic acid (UDCA).
4 . The cocrystalline form of claim 1 , wherein the bile acid is chenodeoxycholic acid (CDCA).
5 . The cocrystalline form of claim 3 , wherein the ratio of obeticholic acid to ursodeoxycholic acid is 2:1.
6 . The cocrystalline form of claim 3 , wherein the ratio of obeticholic acid to ursodeoxycholic acid is 1:1.
7 . The cocrystalline form of claim 3 , wherein the ratio of obeticholic acid to ursodeoxycholic acid is 1:0.5.
8 . The cocrystalline form of claim 3 , characterized by a DSC having an endotherm with an onset at about 174° C.
9 . The cocrystalline form of claim 5 , characterized by having X-ray powder diffraction (XRPD) comprising peaks at approximately at 7.4, 13.8, 14.9, 16.7 and 17.8 degrees 2-theta using Cu Kα radiation.
10 . The cocrystalline form of claim 9 , characterized by having X-ray powder diffraction (XRPD) comprising peaks at approximately 9.5, 15.2, 17.7, 24.7 degrees 2-theta using Cu Kα radiation.
11 . The cocrystalline form of claim 10 , characterized by having X-ray powder diffraction (XRPD) further comprising peaks at approximately 3.6, 8.3, 8.7, 10.3, 10.9, 11.2, 11.9, and 12.8 degrees 2-theta using Cu Kα radiation.
12 . The cocrystalline form of claim 11 , characterized by having X-ray powder diffraction (XRPD) further comprising peaks at approximately 16.8, 16.9, 17.9, 19.3, 19.8, 20.4, 20.7, 21.0, 22.3, 22.7, 23.0, 23.3, and 24.3 degrees 2-theta using Cu Kα radiation.
13 . A cocrystalline form characterized by having X-ray powder diffraction (XRPD) comprising peaks at approximately 3.6, 7.4, 8.3, 8.7, 9.5, 10.3, 10.9, 11.2, 11.9, 12.8, 13.8, 14.9, 15.2, 16.7, 16.8, 16.9, 17.7, 17.8, 17.9, 19.3, 19.8, 20.4, 20.7, 21.0, 22.3, 22.7, 23.0, 23.3, 24.3, 24.7 degrees 2-theta using Cu Kα radiation.
14 . The cocrystalline form of claim 5 , characterized by having a monoclinic crystal system with the following unit cell parameters: a=approximately 24.19 Å, b=approximately 11.88 Å, and c=approximately 25.59 Å.
15 . The cocrystalline form of claim 1 characterized by stability on storage at 40° C./75% RH and 25° C./97% RH.
16 . A pharmaceutical composition comprising the cocrystalline form of any one of claims 1 - 15 , and a pharmaceutically acceptable diluent, excipient or carrier.
17 . A method of treating or preventing an FXR-mediated disease or disorder in a subject in need thereof, comprising administering a therapeutically effective amount of the cocrystalline form of any one of claims 1 - 15 .
18 . A method of modulating FXR activity in a subject in need thereof, comprising administering a therapeutically effective amount of the cocrystalline form of any one of claims 1 - 15 .
19 . A method of preparing the cocrystalline form of claim 1 , comprising:
(a) dissolving OCA and the co-former in a solvent to form a solution;
(b) optionally heating the resulted solution;
(c) cooling the solution and optionally applying an anti-solvent; and
(f) filtering the product from step (c) and drying the product under vacuum.
20 . The method of claim 19 , wherein the solvent is acetonitrile.
21 . The method of claim 19 , wherein the solvent is tetrahydrofuran and the anti-solvent is heptane.