IP Library › Granted Patent US 12,344,847
Granted Patent B2
US 12,344,847 · App. 16/493,245 · Granted Jul 1, 2025

Cell-associated heterologous food and/or feed enzymes

Inventors: Aaron Argyros (Lebanon, NH); Michelle Oeser (Croydon, NH); Erin Wiswall (Danbury, NH); Janet Fisher (Enfield, VT); Johannes Van Eijk (Longueuil, CA); J. Kevin Kraus (Tenafly, NJ); Kevin Wenger (Hanover, NH); Brooks Henningsen (Salisbury, NH); Ryan Skinner (South Royalton, VT)
Assignee: DANSTAR FERMENT AG
C12N15/81A21D8/047A23K10/18A23K20/189A23L33/14C12N1/18C12N9/16C12N9/242C12N9/2428C12Y302/01133
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Quick Facts
Patent No.
US 12,344,847
App. No.
16/493,245
Granted
Jul 1, 2025
Kind
B2
Abstract

The present disclosure concerns recombinant yeast host cells expressing cell-associated heterologous food and/or feed enzymes which are expressed during the propagation phase of the recombinant yeast hosts cells. The recombinant yeast host cells can be used in a subsequent production process to make food and/or feed products, for example, baked products.

Claims (23)

1. A process for making a food or a feed product, said process comprising including a recombinant yeast host cell or an additive comprising the recombinant yeast host cell in the food or the feed product, wherein the recombinant yeast host cell has a heterologous nucleic acid molecule encoding a cell-associated heterologous maltogenic alpha-amylase, wherein the maltogenic alpha-amylase has at least 80% identity to the amino acid sequence of SEQ ID NO: 1, 51, 65 or 108, wherein the heterologous nucleic acid molecule is operatively associated with a promoter allowing expression of the heterologous nucleic acid molecule during propagation under aerobic conditions.

2. The process of claim 1 , wherein the heterologous nucleic acid molecule allows intracellular expression of the heterologous maltogenic alpha-amylase.

3. The process of claim 1 , wherein the heterologous nucleic acid molecule allows expression of a membrane-associated heterologous maltogenic alpha-amylase.

4. The process of claim 1 , wherein the heterologous nucleic acid molecule allows expression of a tethered heterologous maltogenic alpha-amylase.

5. The process of claim 4 , wherein the tethered heterologous maltogenic alpha-amylase is a chimeric protein of formula (I) or (II):

(NH 2 )FFE-L-TT(COOH)  (I)

(NH 2 )TT-L-FFE(COOH)  (II)

wherein FFE is the maltogenic alpha-amylase;

L is present or absent and is an amino acid linker;

TT is an amino acid tethering moiety for associating the maltogenic alpha-amylase to a cell wall of the recombinant yeast host cell;

(NH2) indicates the amino terminus of the chimeric protein;

(COOH) indicates the carboxyl terminus of the chimeric protein; and

“-” is an amide linkage.

6. The process of claim 1 , wherein the promoter is a heterologous promoter.

7. The process of claim 6 , wherein the heterologous promoter comprises the promoter from the tdh1 gene, the hor7 gene, the hsp150 gene, the hxt7 gene, the gpm1 gene, the pgk1 gene and/or the stl1 gene.

8. The process of claim 1 , wherein the recombinant yeast host cell is from the genus Saccharomyces sp.

9. The process of claim 1 , wherein the recombinant yeast host cell is from the species Saccharomyces cerevisiae.

10. The process of claim 1 , wherein the additive is a food additive, a dough conditioner, or a feed additive.

11. The process of claim 1 , further comprising fermenting the food or the feed product with the recombinant yeast host cell present, and/or baking the food or the feed product to provide a baked product.

12. The process of claim 11 , wherein the baked product is a bread.

13. The process of claim 3 , wherein the membrane-associated heterologous maltogenic alpha-amylase has a heterologous signal peptide.

14. The process of claim 4 , wherein the tethered heterologous maltogenic alpha-amylase has a heterologous signal peptide.

15. The process of claim 1 , wherein the promoter is a native promoter.

Assignments (2)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jul 26, 2024
From: LALLEMAND HUNGARY LIQUIDITY MANAGEMENT LLC
To: DANSTAR FERMENT AG
Reel/Frame 068174/0430 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Dec 9, 2019
From: ARGYROS, AARON; OESER, MICHELLE; WISWALL, ERIN; FISHER, JANET; VAN EIJK, JOHANNES; KRAUS, J. KEVIN; WENGER, KEVIN; HENNINGSEN, BROOKS; SKINNER, RYAN
To: LALLEMAND HUNGARY LIQUIDITY MANAGEMENT LLC
Reel/Frame 051219/0305 →
Continuity (3)
Provisional Application 62470568 · Mar 13, 2017
Provisional Application 62625751 · Feb 2, 2018
Related Publication 20200087672A1 · Mar 19, 2020
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