TRANSGENIC RAINBOW SHARK
The present invention relates to transgenic ornamental fish, as well as methods of making such fish by in vitro fertilization techniques. Also disclosed are methods of establishing a population of such transgenic fish and methods of providing them to the ornamental fish industry for the purpose of marketing.
1 . A transgenic rainbow shark comprising a chromosomally integrated expression cassette encoding a fluorescent protein, wherein the rainbow shark comprises the “Orange rainbow shark 1 transformation event,” sperm comprising the Orange rainbow shark 1 transformation event being deposited at the ECACC.
2 . The transgenic rainbow shark of claim 1 , further defined as a fertile, transgenic rainbow shark.
3 . The transgenic rainbow shark of claim 1 , wherein the fish is homozygous for the integrated expression cassette.
4 . The transgenic rainbow shark of claim 1 , wherein the fish is heterozygous for the integrated expression cassette.
5 . A method of providing a transgenic rainbow shark to the ornamental fish market, comprising obtaining a transgenic rainbow shark in accordance with claim 1 , and distributing the fish to the ornamental fish market.
6 . The method of claim 5 , wherein the fish are distributed by a grower to a commercial distributor.
7 . The method of claim 5 , wherein the fish are distributed by a grower or a commercial distributor to a retailer.
8 . The method of claim 7 , wherein the retailer is a multi-product retailer having an ornamental fish department.
9 . A method of producing a transgenic rainbow shark comprising:
(a) obtaining a rainbow shark that comprises a chromosomally integrated expression cassette encoding a fluorescent protein, wherein the rainbow shark comprises the “Orange rainbow 1 transformation event,” sperm comprising the Orange rainbow shark 1 transformation event being deposited at the ECACC; and
(b) breeding the obtained rainbow shark with a second rainbow shark to provide a transgenic rainbow shark comprising the Orange rainbow shark 1 transformation event.
10 . The method of claim 9 , wherein the second rainbow shark is a non-transgenic rainbow shark.
11 . A progeny of a transgenic rainbow shark of claim 1 that comprises a chromosomally integrated expression cassette encoding a fluorescent protein, wherein the rainbow shark and progeny exhibit fluorescence and comprise the “Orange rainbow shark 1 transformation event,” sperm comprising the Orange rainbow shark 1 transformation event being deposited at the ECACC.
12 . The progeny fish of claim 11 , further defined as a fertile, transgenic rainbow shark.
13 . The progeny fish of claim 11 , wherein the fish is homozygous for the integrated expression cassette.
14 . The progeny fish of claim 11 , wherein the fish is heterozygous for the integrated expression cassette.
15 . A method of providing a transgenic fish to the ornamental fish market, comprising obtaining a progeny fish in accordance with claim 11 , and distributing the fish to the ornamental fish market.
16 . The method of claim 15 , wherein the fish are distributed by a grower to a commercial distributor.
17 . The method of claim 16 , wherein the fish are distributed by a grower or a commercial distributor to a retailer.
18 . The method of claim 17 , wherein the retailer is a multi-product retailer having an ornamental fish department.
19 . A method of producing a transgenic fish comprising:
(a) obtaining a transgenic fish in accordance with claim 11 ; and
(b) breeding the obtained fish with a second fish to provide a transgenic fish comprising the Orange rainbow shark 1 transformation event.
20 . The method of claim 19 , wherein the second fish is a non-transgenic fish.
21 . A transgenic rainbow shark comprising a chromosomally integrated expression cassette encoding a fluorescent protein, wherein the rainbow shark comprises the “Purple rainbow shark 1 transformation event,” sperm comprising the Purple rainbow shark 1 transformation event being deposited at the ECACC.
22 . The transgenic rainbow shark of claim 21 , further defined as a fertile, transgenic rainbow shark.
23 . The transgenic rainbow shark of claim 21 , wherein the fish is homozygous for the integrated expression cassette.
24 . The transgenic rainbow shark of claim 21 , wherein the fish is heterozygous for the integrated expression cassette.
25 . A method of providing a transgenic rainbow shark to the ornamental fish market, comprising obtaining a transgenic rainbow shark in accordance with claim 21 , and distributing the fish to the ornamental fish market.
26 . The method of claim 25 , wherein the fish are distributed by a grower to a commercial distributor.
27 . The method of claim 25 , wherein the fish are distributed by a grower or a commercial distributor to a retailer.
28 . The method of claim 27 , wherein the retailer is a multi-product retailer having an ornamental fish department.
29 . A method of producing a transgenic rainbow shark comprising:
(a) obtaining a rainbow shark that comprises a chromosomally integrated expression cassette encoding a fluorescent protein, wherein the rainbow shark comprises the “Purple rainbow 1 transformation event,” sperm comprising the Purple rainbow shark 1 transformation event being deposited at the ECACC; and
(b) breeding the obtained rainbow shark with a second rainbow shark to provide a transgenic rainbow shark comprising the Purple rainbow shark 1 transformation event.
30 . The method of claim 29 , wherein the second rainbow shark is a non-transgenic rainbow shark.
31 . A progeny of a transgenic rainbow shark of claim 21 that comprises a chromosomally integrated expression cassette encoding a fluorescent protein, wherein the rainbow shark and progeny exhibit fluorescence and comprise the “Purple rainbow shark 1 transformation event,” sperm comprising the Purple rainbow shark 1 transformation event being deposited at the ECACC.
32 . The progeny fish of claim 31 , further defined as a fertile, transgenic rainbow shark.
33 . The progeny fish of claim 31 , wherein the fish is homozygous for the integrated expression cassette.
34 . The progeny fish of claim 31 , wherein the fish is heterozygous for the integrated expression cassette.
35 . A method of providing a transgenic fish to the ornamental fish market, comprising obtaining a progeny fish in accordance with claim 34 , and distributing the fish to the ornamental fish market.
36 . The method of claim 35 , wherein the fish are distributed by a grower to a commercial distributor.
37 . The method of claim 35 , wherein the fish are distributed by a grower or a commercial distributor to a retailer.
38 . The method of claim 37 , wherein the retailer is a multi-product retailer having an ornamental fish department.
39 . A method of producing a transgenic fish comprising:
(a) obtaining a transgenic fish in accordance with claim 31 ; and
(b) breeding the obtained fish with a second fish to provide a transgenic fish comprising the Purple rainbow shark 1 transformation event.
40 . The method of claim 39 , wherein the second fish is a non-transgenic fish.
41 . A transgenic rainbow shark comprising a chromosomally integrated expression cassette encoding a fluorescent protein, wherein the rainbow shark comprises the “Blue rainbow shark 1 transformation event,” sperm comprising the Blue rainbow shark 1 transformation event being deposited at the ECACC.
42 . The transgenic rainbow shark of claim 41 , further defined as a fertile, transgenic rainbow shark.
43 . The transgenic rainbow shark of claim 41 , wherein the fish is homozygous for the integrated expression cassette.
44 . The transgenic rainbow shark of claim 41 , wherein the fish is heterozygous for the integrated expression cassette.
45 . A method of providing a transgenic rainbow shark to the ornamental fish market, comprising obtaining a transgenic rainbow shark in accordance with claim 41 , and distributing the fish to the ornamental fish market.
46 . The method of claim 45 , wherein the fish are distributed by a grower to a commercial distributor.
47 . The method of claim 45 , wherein the fish are distributed by a grower or a commercial distributor to a retailer.
48 . The method of claim 47 , wherein the retailer is a multi-product retailer having an ornamental fish department.
49 . A method of producing a transgenic rainbow shark comprising:
(a) obtaining a rainbow shark that comprises a chromosomally integrated expression cassette encoding a fluorescent protein, wherein the rainbow shark comprises the “Blue rainbow 1 transformation event,” sperm comprising the Blue rainbow shark 1 transformation event being deposited at the ECACC; and
(b) breeding the obtained rainbow shark with a second rainbow shark to provide a transgenic rainbow shark comprising the Blue rainbow shark 1 transformation event.
50 . The method of claim 49 , wherein the second rainbow shark is a non-transgenic rainbow shark.
51 . A progeny of a transgenic rainbow shark of claim 41 that comprises a chromosomally integrated expression cassette encoding a fluorescent protein, wherein the rainbow shark and progeny exhibit fluorescence and comprise the “Blue rainbow shark 1 transformation event,” sperm comprising the Blue rainbow shark 1 transformation event being deposited at the ECACC.
52 . The progeny fish of claim 51 , further defined as a fertile, transgenic rainbow shark.
53 . The progeny fish of claim 51 , wherein the fish is homozygous for the integrated expression cassette.
54 . The progeny fish of claim 51 , wherein the fish is heterozygous for the integrated expression cassette.
55 . A method of providing a transgenic fish to the ornamental fish market, comprising obtaining a progeny fish in accordance with claim 51 , and distributing the fish to the ornamental fish market.
56 . The method of claim 55 , wherein the fish are distributed by a grower to a commercial distributor.
57 . The method of claim 56 , wherein the fish are distributed by a grower or a commercial distributor to a retailer.
58 . The method of claim 57 , wherein the retailer is a multi-product retailer having an ornamental fish department.
59 . A method of producing a transgenic fish comprising:
(a) obtaining a transgenic fish in accordance with claim 51 ; and
(b) breeding the obtained fish with a second fish to provide a transgenic fish comprising the Blue rainbow shark 1 transformation event.
60 . The method of claim 59 , wherein the second fish is a non-transgenic fish.
61 . A transgenic rainbow shark comprising a chromosomally integrated expression cassette encoding a fluorescent protein, wherein the rainbow shark comprises the “Green rainbow shark 1 transformation event,” sperm comprising the Green rainbow shark 1 transformation event being deposited at the ECACC.
62 . The transgenic rainbow shark of claim 61 , further defined as a fertile, transgenic rainbow shark.
63 . The transgenic rainbow shark of claim 61 , wherein the fish is homozygous for the integrated expression cassette.
64 . The transgenic rainbow shark of claim 61 , wherein the fish is heterozygous for the integrated expression cassette.
65 . A method of providing a transgenic rainbow shark to the ornamental fish market, comprising obtaining a transgenic rainbow shark in accordance with claim 61 , and distributing the fish to the ornamental fish market.
66 . The method of claim 65 , wherein the fish are distributed by a grower to a commercial distributor.
67 . The method of claim 65 , wherein the fish are distributed by a grower or a commercial distributor to a retailer.
68 . The method of claim 67 , wherein the retailer is a multi-product retailer having an ornamental fish department.
69 . A method of producing a transgenic rainbow shark comprising:
(a) obtaining a rainbow shark that comprises a chromosomally integrated expression cassette encoding a fluorescent protein, wherein the rainbow shark comprises the “Green rainbow 1 transformation event,” sperm comprising the Green rainbow shark 1 transformation event being deposited at the ECACC; and
(b) breeding the obtained rainbow shark with a second rainbow shark to provide a transgenic rainbow shark comprising the Green rainbow shark 1 transformation event.
70 . The method of claim 69 , wherein the second rainbow shark is a non-transgenic rainbow shark.
71 . A progeny of a transgenic rainbow shark of claim 61 that comprises a chromosomally integrated expression cassette encoding a fluorescent protein, wherein the rainbow shark and progeny exhibit fluorescence and comprise the “Green rainbow shark 1 transformation event,” sperm comprising the Green rainbow shark 1 transformation event being deposited at the ECACC.
72 . The progeny fish of claim 71 , further defined as a fertile, transgenic rainbow shark.
73 . The progeny fish of claim 71 , wherein the fish is homozygous for the integrated expression cassette.
74 . The progeny fish of claim 71 , wherein the fish is heterozygous for the integrated expression cassette.
75 . A method of providing a transgenic fish to the ornamental fish market, comprising obtaining a progeny fish in accordance with claim 71 , and distributing the fish to the ornamental fish market.
76 . The method of claim 75 , wherein the fish are distributed by a grower to a commercial distributor.
77 . The method of claim 76 , wherein the fish are distributed by a grower or a commercial distributor to a retailer.
78 . The method of claim 77 , wherein the retailer is a multi-product retailer having an ornamental fish department.
79 . A method of producing a transgenic fish comprising:
(a) obtaining a transgenic fish in accordance with claim 71 ; and
(b) breeding the obtained fish with a second fish to provide a transgenic fish comprising the Green rainbow shark 1 transformation event.
80 . The method of claim 79 , wherein the second fish is a non-transgenic fish.