Red cell diluent with EDTA and methods for making and using the same
The present disclosure relates to a red blood cell (RBC) solution comprising ethylenediaminetetraacetic acid (EDTA) and methods for making and using the same. The solution can comprise EDTA at a concentration from 3-5 g/L, including 4 g/L. Well plates prepared using the solution can provide a decreased rate of false positive results in automated immunoassays for detecting RBC antibodies in a patient sample.
1. A method of preparing a well comprising:
preparing a RBC solution by suspending packed red blood cells (RBCs) in a solution comprising ethylenediaminetetraacetic acid (EDTA); and
applying the RBC solution to a well to allow the RBCs in the RBC solution to attach to the surface of the well, thereby immobilizing RBCs in the well, and
treating the immobilized RBCs with a lysing agent such that fragments of the RBCs remain immobilized in the well.
2. The method of claim 1 , wherein the well comprises an attachment molecule bound to a surface of the well and the RBCs are immobilized in the well by the attachment molecule.
3. The method of claim 1 , further comprising washing the well to remove cellular debris.
4. The method of claim 3 , further comprising treating the well with a drying solution.
5. The method of claim 4 , wherein the well is stored for at least three days following treatment with the drying solution.
6. The method of claim 1 , wherein the treating with the lysing agent is performed at least twenty-four hours after applying the RBC solution to the well.
7. The method claim 1 , wherein the well surface comprises polystyrene.
8. The method claim 1 , wherein the solution comprises EDTA at a concentration from 3-5 g/L.
9. The method of claim 8 , wherein the concentration is 4 g/L.
10. The method of claim 1 , wherein the EDTA is one or more of disodium EDTA and tetrasodium EDTA.