Antibodies to paliperidone haptens and use thereof
Disclosed is an antibody which binds to paliperidone, which can be used to detect paliperidone in a sample such as in a competitive immunoassay method. The antibody can be used in a lateral flow assay device for point-of-care detection of paliperidone, including multiplex detection of aripiprazole, olanzapine, quetiapine, risperidone and paliperidone in a single lateral flow assay device.
1. A conjugate of a compound of Formula I and an immunogenic carrier, Formula I:
wherein
L 1 is OC(O)(CH 2 ) n , or O(CH 2 ) n ;
wherein n is 2 or 3;
L 2 is NHC(O),
or absent; and
L 3 is (CH 2 ) m CO 2 H, or
wherein m is 0, 1, 2, or 3; provided that m may only be 0 if L 2 is absent; and further provided that when L 2 is absent L 3 is
2. The conjugate of claim 1 , wherein the immunogenic carrier is a protein.
3. The conjugate of claim 2 , wherein the protein is keyhole limpet hemocyanin, bovine thyroglobulin, or ovalbumin.
4. A conjugate of an immunogenic carrier and a compound selected from the group consisting of:
wherein n is 2 or 3; and m 0, 1, 2 or 3.
5. The conjugate of claim 4 , wherein the compound is
6. The conjugate of claim 4 , wherein the compound is
7. The conjugate of claim 4 , wherein the immunogenic carrier is a protein.
8. The conjugate of claim 7 , wherein the protein is keyhole limpet hemocyanin, bovine thyroglobulin, or ovalbumin.
9. A method of preparing a conjugate comprising contacting an immunogenic carrier with a compound selected from the group consisting of:
wherein n is 2 or 3; and m 0, 1, 2 or 3.
10. The method of claim 9 , wherein the immunogenic carrier is a protein.
11. The method of claim 10 , wherein the protein is keyhole limpet hemocyanin, bovine thyroglobulin, or ovalbumin.