Universal chimeric antigen expressing immune cells for targeting of diverse multiple antigens and method of manufacturing the same and use of the same for treatment of cancer, infections and autoimmune disorders
The present invention relates to immune cell-based anti-cancer therapeutics and methods of using the therapeutics in the treatment of cancer.
1. An antibody or fragment thereof that specifically binds CD33, wherein said antibody or antibody fragment comprises a single chain variable fragment (scFV) that comprises a humanized light chain variable domain comprising the amino acid sequence of SEQ ID NO:40 and a humanized heavy chain variable domain comprising the amino acid sequence of SEQ ID NO:41, wherein said light chain variable domain and said heavy chain variable domain are connected by a linker peptide that is about 10 to 25 amino acids in length.
2. The antibody or antibody fragment of claim 1 wherein the N-terminus of the amino acid sequence of SEQ ID NO:40 is connected to the C-terminus of the amino acid sequence of SEQ ID NO:41.
3. The antibody or antibody fragment of claim 1 wherein said linker peptide comprises the amino acid sequence of amino acids 413-427 of SEQ ID NO. 32.
4. The antibody or antibody fragment of claim 1 further comprising a second scFV against a second antigen.
5. A nucleic acid encoding the antibody or antibody fragment of claim 1 .
6. The nucleic acid of claim 5 , comprising the nucleotide sequence of SEQ ID NO. 21 and the nucleotide sequence of SEQ ID NO. 22.
7. A pharmaceutical composition comprising the antibody or antibody fragment of claim 1 and a pharmaceutically acceptable excipient.
8. A pharmaceutical composition comprising the antibody or antibody fragment of claim 4 and a pharmaceutically acceptable excipient.
9. The antibody or antibody fragment of claim 1 wherein said linker peptide comprises the amino acid sequence of amino acids 135-159 of SEQ ID NO. 30.