Disease resistance alleles in soybean
The present invention relates to methods and compositions for identifying, selecting and/or producing a soybean plants having increased resistance to bacterial pustule disease. A soybean plant or germplasm that has been identified, selected and/or produced by any of the methods of the present invention is also provided. Disease resistant soybean seeds, plants and germplasms are also provided.
1. A method of producing a soybean plant having increased resistance to bacterial pustule, the method comprising the steps of:
a. isolating a nucleic acid from a soybean plant part;
b. detecting in the nucleic acid of a) at least one molecular marker that is associated with increased bacterial pustule resistance, wherein said molecular marker is localized within 1 cM of an allele corresponding to a Tat position 251 of SEQ ID NO: 5;
c. selecting or identifying a soybean plant on the basis of the presence of said molecular marker of b);
d. crossing the soybean plant of c) with a second soybean plant not comprising the molecular marker of b); and
e. producing progeny plant from the cross of d), thereby producing a soybean plant having increased resistance to bacterial pustule.
2. The method of claim 1 , wherein the marker is located within a chromosome interval corresponding to Glycine max chromosome 6 between and including physical positions 49139963 to 49974519.
3. The method of claim 1 , wherein detecting comprises: a) admixing an amplification primer or amplification primer pair with a nucleic acid isolated from a soybean plant or soybean germplasm, wherein the primer or primer pair is complementary or partially complementary to at least a portion of the marker locus, and is capable of initiating DNA polymerization by a DNA polymerase using the soybean nucleic acid as a template; and, b) extending the primer or primer pair in a DNA polymerization reaction comprising a DNA polymerase and a template nucleic acid to generate at least one informative fragment wherein the informative fragment comprises SEQ ID NO: 5.
4. The method of claim 1 , wherein the progeny plant is an elite soybean plant.
5. The method of claim 3 , wherein the primer pair comprises the SEQ ID NO pair: 24 and 25.
6. The method of claim 1 , wherein the molecular marker is a single nucleotide polymorphism (SNP), a quantitative trait locus (QTL), an amplified fragment length polymorphism (AFLP), randomly amplified polymorphic DNA (RAPD), a restriction fragment length polymorphism (RFLP) or a microsatellite.