Method for the fermentative production of L-lysine
A novel method is used for the fermentative production of L-lysine using bacteria of the species Corynebacterium glutamicum , having the ability to excrete L-lysine, containing in their chromosome a variant of a polynucleotide encoding a polypeptide having hydrolase activity. The bacteria are cultivated in a suitable medium under suitable conditions, and then L-lysine is accumulated in the medium to form an L-lysine containing fermentation broth.
1 . A method for the fermentative production of L-lysine, comprising:
a) providing a bacterium of the species Corynebacterium glutamicum having an ability to excrete L-lysine, containing in its chromosome a polynucleotide encoding a polypeptide comprising an amino acid sequence of SEQ ID NO:4,
b) cultivating the bacterium in a suitable medium under suitable conditions, and
c) accumulating said L-lysine in the suitable medium to form an L-lysine containing fermentation broth.
2 . The method as claimed in claim 1 , wherein in the bacterium provided, said polynucleotide, encoding said amino acid sequence, comprises a nucleotide sequence of SEQ ID NO:3 positions 923 to 2812.
3 . The method as claimed in claim 1 , wherein in the bacterium provided, the polynucleotide, encoding said amino acid sequence, comprises a nucleotide sequence of SEQ ID NO:3 positions 923 to 2815.
4 . The method as claimed in claim 1 , wherein in the bacterium provided, the polynucleotide, encoding said amino acid sequence, comprises a nucleotide sequence of SEQ ID NO:3 positions 585 to 2815.
5 . The method as claimed in claim 1 , wherein in the bacterium provided, the polynucleotide, encoding said amino acid sequence, comprises a nucleotide sequence of SEQ ID NO:3 positions 565 to 2815.