Combination of a DNA condensation-inducing compound and an Eis inhibitor for antibiotic treatment
View Patent ↗A method of killing bacteria and an antibiotic kit are provided herein. The method includes treating the bacteria with a DNA condensation-inducing compound; and subsequently treating the bacteria with an Eis inhibitor. The kit includes a DNA condensation-inducing compound packaged together with an Eis inhibitor.
1. A method of killing bacteria, comprising:
treating the bacteria with a DNA condensation-inducing compound; and
subsequently treating the bacteria with an Eis inhibitor.
2. The method of claim 1 , wherein the DNA condensation-inducing compound is an antibiotic.
3. The method of claim 2 , wherein the antibiotic is selected from the group consisting of: fusidic acid, nalidixic acid, linezolid, streptomycin, and rifampicin.
4. The method of claim 1 , wherein the Eis inhibitor is a pyrrolo[1,5-a]pyrazine-based Eis inhibitor having the following structure:
wherein R 1 is selected from the group consisting of H, p-F, and m,p-di-F; and
wherein R 2 is selected from the group consisting of H, o-F, m-F, p-F, m-Cl, p-Cl, m-Br, p-Br, p Me, and m-OMe.
5. The method of claim 1 , wherein the bacteria are mycobacteria.
6. The method of claim 1 , wherein the bacteria include Mycobacterium tuberculosis.
7. The method of claim 1 , wherein the bacteria include Mycobacterium smegmatis.
8. The method of claim 1 , wherein the bacteria are causing an infection in a subject.
9. The method of claim 8 , wherein the step of treating the bacteria with a DNA condensation-inducing compound includes administering the DNA condensation-inducing compound to the subject.
10. The method of claim 9 , wherein the step of subsequently treating the bacteria with the Eis inhibitor includes administering the Eis inhibitor to the subject.
11. An antibiotic kit, comprising: a DNA condensation-inducing compound packaged together with an Eis inhibitor.
12. The kit of claim 11 , wherein the DNA condensation-inducing compound is an antibiotic.
13. The kit of claim 12 , wherein the antibiotic is selected from the group consisting of: fusidic acid, nalidixic acid, linezolid, streptomycin, and rifampicin.
14. The kit of claim 11 , wherein the Eis inhibitor is a pyrrolo[1,5-a]pyrazine-based Eis inhibitor having the following structure:
wherein R 1 is selected from the group consisting of H, p-F, and m,p-di-F; and
wherein R 2 is selected from the group consisting of H, o-F, m-F, p-F, m-Cl, p-Cl, m-Br, p-Br, p Me, and m-OMe.
15. The kit of claim 11 , further comprising instructions for killing bacteria.
16. The kit of claim 15 , wherein the bacteria are mycobacteria.
17. The kit of claim 15 , wherein the bacteria include Mycobacterium tuberculosis.
18. The kit of claim 15 , wherein the bacteria include Mycobacterium smegmatis.
19. The kit of claim 15 , wherein the bacteria are causing an infection in a subject.
20. The kit of claim 15 , wherein the instructions comprise administering the DNA condensation-inducing compound to the subject, and subsequently administering the Eis inhibitor to the subject.