Method of Producing a Mycological Product and Product Made Thereby
A panel of mycological polymer consisting entirely of fungal mycelium as described in U.S. patent application Ser. No. 16/190,585 is post-processed to impart desired characteristics thereto, such as, texture, flavor and nutritional profile for use as a foodstuff or a tissue scaffold. Alternatively, the growth conditions of the growth media may be tailored to obtain a desired density, morphology, and/or composition of the undifferentiated fungal material with or without the use of post-processes.
1 . A method of producing a mycological product comprising the steps of
growing a porous tissue of a mycological polymer consisting entirely of fungal mycelium on a growth media comprised of nutritive substrate and a fungus while preventing full differentiation of said fungus into a mushroom;
removing a panel of mycological polymer from said porous tissue; and
packaging said panel for use.
2 . A method as set forth in claim 1 wherein said growth media is composed of 15% crude protein, 33% non-fiber carbohydrates, 28% lignin and 14% crude fat.
3 . A method as set forth in claim 1 further comprising the step of infusing said panel with at least one additive selected form the group consisting of plant-derived proteins, fats, micronutrients and desired flavoring ingredients to mimic animal-derived meat products in said panel.
4 . A method as set forth in claim 3 wherein said step of infusing is performed under vacuum.
5 . A method as set forth in claim 3 wherein said step of infusing comprises soaking of said panel with said at least one additive.
6 . A method as set forth in claim 3 wherein said at least one additive is one of a plant-derived additive, a cell derived additive, a fermented bacterial or fungal derived additive and an animal derived additive
7 . A method as set forth in claim 1 further comprising the step of including elevated levels of essential dietary minerals in said growth media for bioaccumulating in said panel.
8 . A method as set forth in claim 4 further comprising the step of adding flavoring additives to said panel by one of soaking and vacuum infusion.
9 . A method as set forth in claim 1 further comprising the step of adding blocking compounds to said growth media to increase shelf-life in said panel.
10 . A method as set forth in claim 6 further comprising the step of washing said panel in dilute hydrogen peroxide (3.5%) and drying under vacuum at a temperature and pressure to remove known malodors.
11 . A method as set forth in claim 1 further comprising the steps of mechanically tenderizing said panel.
12 . A method as set forth in claim 11 wherein said step of tenderizing includes passing an array of pins into said panel.
13 . A method as set forth in claim 12 further comprising the step of thereafter placing said panel in a chitinase bath to further tenderize said panel.
14 . A method as set forth in claim 1 further comprising the steps of:
decellularizing said panel in a heated SDS bath with sonication;
therefafter sterilizing said decellularized panel; and
inoculating said sterilized panel with bovine myocytes in a bath of fetal bovine serum to form a complete three-dimensional cellular structure in vitro.
15 . A panel of mycological biopolymer consisting entirely of fungal mycelium with an additive of at least one of plant-derived proteins, fats, micronutrients and selected flavoring ingredients therein.
16 . A panel as set forth in claim 15 wherein said additive mimics animal-derived meat products in said panel.
17 . A panel as set forth in claim 15 further comprising inoculated bovine myocytes therein.
18 . A panel as set forth in claim 15 further comprising blocking compounds for increasing shelf-life in said panel