Systems and methods for detecting particles in a fluid channel
Disclosed herein are systems and methods capable of identifying, tracking, and sorting particles or droplets flowing in a channel, for example, a microfluidic channel having a fluid medium. The channel and the fluid medium can have a similar refractive index such that they appear translucent or transparent when illuminated by electromagnetic radiation. The particles or droplets can have a refractive index substantially different from that of the channel and the medium, such that the particles or droplets interfere with the electromagnetic radiation. A sensor can be disposed adjacent to the channel to record the electromagnetic radiation. The sensor can be attached to a system for identifying, tracking, and sorting the droplets.
1. A system for detecting a particle in a channel, comprising:
a substrate;
a channel positioned in or on the substrate, the channel comprising at least one particle dispersed in a medium, such that the at least one particle is moving from a first end of the channel to a second end of the channel;
a source of electromagnetic radiation that illuminates at least a portion of the channel;
a first sensor to detect the at least one particle, wherein the first sensor is positioned in line with a linear axis of the illuminated portion of the channel such that the first sensor is parallel to a direction of movement of the at least one particle through the channel;
a second sensor positioned within the illuminated portion of the channel, wherein the second sensor is perpendicular to the linear axis of the channel; and
an optical system that focuses and aligns the illuminated portion of the channel to the first sensor;
wherein the substrate, the channel, and the medium have a similar refractive index.
2. The system of claim 1 , wherein the first sensor and the second sensor comprises a linear charge-coupled sensor, a linear complementary metal-oxide-semiconductor sensor, or any combination thereof.
3. The system of claim 1 , wherein the source of electromagnetic radiation comprises another optical system.
4. The system of claim 1 , comprising a plurality of particles and/or a plurality of channels and/or a plurality of linear sensors.
5. The system of claim 1 , further comprising a detector coupled to the first sensor, wherein the detector detects movement and/or position of the at least one particle over a predetermined time and/or distance.
6. The system of claim 1 , wherein the optical system comprises a lens selected from the group consisting of a cylindrical lens, or a telecentric lens, or a spherical lens, or an aspheric lens, or any combination thereof.
7. The system of claim 1 , wherein the first sensor detects spectral information about the particle.
8. The system of claim 1 , wherein the at least one particle is a droplet.
9. The system of claim 1 , wherein signals from the first sensor are used to activate a device.
10. The system of claim 9 , wherein the device is a component for sorting a plurality of particles in a channel based on size, position and/or other characteristics.
11. A method of detecting a particle in a channel comprising:
providing a substrate comprising a channel;
allowing at least one particle to flow through the channel, the channel having a linear axis perpendicular to a circumference of the channel;
aligning a linear sensor in-line with the linear axis of the channel such that the linear sensor is parallel to a direction of movement of the at least one particle through the channel;
illuminating the channel with electromagnetic radiation along at least the linear axis, wherein the at least one particle changes optical properties of the electromagnetic radiation transmitted to the linear sensor;
aligning a second sensor perpendicular to the linear axis of the channel such that the second sensor is perpendicular to a direction of movement of the at least one particle through the channel; and
detecting, with the linear sensor, the electromagnetic radiation as transmitted along the linear axis of the channel, wherein the substrate, the channel, and the medium have a similar refractive index.
12. The method of claim 11 , wherein the detecting is in real time.
13. The method of claim 11 , further comprising characterizing the at least one particle.
14. The method of claim 13 , wherein characterizing the at least one particle comprises identifying the at least one particle, recording a velocity of the at least one particle, recording a size of the at least one particle, recording an absorption spectrum of the at least one particle, recording a fluorescent spectrum of the at least one particle; recording light scattering of the at least one particle, recording a refractive index of the at least one particle, or any combination thereof.
15. The method of claim 13 , further comprising sorting a plurality of particles in real time.
16. The method of claim 15 , wherein the sorting is performed according to particle identification, particle size, or any combination thereof.
17. The method of claim 15 , wherein the sorting is performed by actuating at least an electrode, valve or other component that may be used to change a direction of particle flow through the channel.