Modified Vip3 polypeptides
The present invention is directed to the vegetative insecticidal proteins (Vips) modified to comprise heterologous bacterial carbohydrate binding modules and the methods of use thereof. Expression of modified proteins resulted in variations to activity against lepidopteran pest species of agricultural importance such as Corn earworm and Fall armyworm conveying broad spectrum insect control.
1. A modified Vip3A polypeptide comprising a heterologous carbohydrate binding module (CBM), wherein the CBM is from a bacterial β-1,4-mannanase and is substituted for all or a portion of Domain III of a Vip3A polypeptide, and wherein the modified Vip3A polypeptide comprises all or a portion of SEQ ID NO:2 and is pesticidal against an insect.
2. The modified Vip3A polypeptide of claim 1 , wherein the Vip3A polypeptide comprises a portion of SEQ ID NO:2.
3. The modified Vip3A polypeptide of claim 1 , wherein all or a portion of Domain III comprises amino acids 542 to 667 of SEQ ID NO:2.
4. The modified Vip3A polypeptide of claim 1 , wherein the heterologous CBM comprises a metal binding site.
5. The modified Vip3A polypeptide of claim 1 , wherein the modified Vip3A polypeptide is pesticidal against a lepidopteran insect.
6. The modified Vip3A polypeptide of claim 1 , wherein the modified Vip3A polypeptide is pesticidal against at least one of Ostrinia nubilalis (European corn borer), Plutella xylostella (diamondback moth), Spodoptera frugiperda (fall armyworm), Agrotis ipsilon (black cutworm), Agrotis orthogonia (pale western cutworm), Striacosta albicosta (western bean cutworm), Helicoverpa zea (corn earworm), Heliothis virescens (tobacco budworm), Spodoptera exigua (beet armyworm), Helicoverpa punctigera (native budworm), Helicoverpa armigera (cotton bollworm), Manduca sexta (tobacco hornworm), Trichoplusia ni (cabbage looper), Pectinophora gossypiella (pink bollworm), Diatraea grandiosella (southwestern corn borer), Diatraea saccharalis (sugarcane borer), Elasmopalpus lignosellus (lesser cornstalk borer), Psuedoplusia includens (soybean looper), Anticarsia gemmatalis (velvetbean caterpillar), Plathypena scabra (green cloverworm), Homoeosoma electellum (sunflower head moth), and Cochylis hospes (banded sunflower moth), or any combination thereof.
7. The modified Vip3A polypeptide of claim 1 , wherein the heterologous CBM is SEQ ID NO:29.
8. The modified Vip3A polypeptide of claim 7 , wherein the modified Vip3A polypeptide comprises SEQ ID NO:21.
9. A composition comprising the modified Vip3A polypeptide of claim 1 in an agriculturally acceptable carrier.