Production of cannabigerolic acid in yeast
Exemplary embodiments provided herein include genetically engineering microorganisms, such as yeast or bacteria, to produce cannabinoids by inserting genes that produce the appropriate enzymes for the metabolic production of a desired compound.
1. A method for making cannabigerolic acid, the method comprising:
transforming a S. cerevisiae with a first nucleotide sequence of SEQ ID NO 1 expressing an acyl-activating enzyme;
transforming the S. cerevisiae with a second nucleotide sequence of SEQ ID NO 22 expressing a mutant prenyltransferase;
transforming the S. cerevisiae with a third nucleotide sequence of SEQ ID NO 10 expressing olivetolic synthase;
transforming the S. cerevisiae with a fourth nucleotide sequence of SEQ ID NO 5 expressing olivetolic acid cyclase; and
transforming the S. cerevisiae with a fifth nucleotide sequence of SEQ ID NO 39 expressing aromatic prenyltransferase.
2. A method for making cannabigerolic acid, the method comprising:
transforming a S. cerevisiae with a first nucleotide sequence of SEQ ID NO 1 expressing an acyl-activating enzyme;
transforming the S. cerevisiae with a second nucleotide sequence of SEQ ID NO 22 expressing a mutant prenyltransferase;
transforming the S. cerevisiae with a third nucleotide sequence of SEQ ID NO 81 expressing olivetolic synthase;
transforming the S. cerevisiae with a fourth nucleotide sequence of SEQ ID NO 5 expressing olivetolic acid cyclase; and
transforming the S. cerevisiae with a fifth nucleotide sequence of SEQ ID NO 39 expressing aromatic prenyltransferase.
3. A method for making cannabigerolic acid, the method comprising:
transforming a S. cerevisiae with a first nucleotide sequence of SEQ ID NO 85 expressing an acyl-activating enzyme;
transforming the S. cerevisiae with a second nucleotide sequence of SEQ ID NO 22 expressing a mutant prenyltransferase;
transforming the S. cerevisiae with a third nucleotide sequence of SEQ ID NO 81 expressing olivetolic synthase;
transforming the S. cerevisiae with a fourth nucleotide sequence of SEQ ID NO 5 expressing olivetolic acid cyclase; and
transforming the S. cerevisiae with a fifth nucleotide sequence of SEQ ID NO 39 expressing aromatic prenyltransferase.
4. A method for making cannabigerolic acid, the method comprising:
transforming a S. cerevisiae with a first nucleotide sequence of SEQ ID NO 23 expressing an acyl-activating enzyme;
transforming the S. cerevisiae with a second nucleotide sequence of SEQ ID NO 24 expressing a mutant prenyltransferase;
transforming the S. cerevisiae with a third nucleotide sequence of SEQ ID NO 25 expressing olivetolic synthase;
transforming the S. cerevisiae with a fourth nucleotide sequence of SEQ ID NO 26 expressing olivetolic acid cyclase; and
transforming the S. cerevisiae with a fifth nucleotide sequence of SEQ ID NO 39 expressing aromatic prenyltransferase.
5. A method for making cannabigerolic acid, the method comprising:
transforming a S. cerevisiae with a first nucleotide sequence of SEQ ID NO 81 expressing an acyl-activating enzyme, olivetolic synthase and olivetolic acid cyclase;
transforming the S. cerevisiae with a second nucleotide sequence of SEQ ID NO 22 expressing a mutant prenyltransferase; and
transforming the S. cerevisiae with a third nucleotide sequence of SEQ ID NO 39 expressing aromatic prenyltransferase.