Kits and devices for detecting analytes
The invention provides devices that improve tests for detecting specific cellular, viral, and molecular targets in clinical, industrial, or environmental samples. The invention permits efficient detection of individual microscopic targets at low magnification for highly sensitive testing. The invention does not require washing steps and thus allows sensitive and specific detection while simplifying manual operation and lowering costs and complexity in automated operation. In short, the invention provides devices that can deliver rapid, accurate, and quantitative, easy-to-use, and cost-effective tests.
1. A testing cartridge comprising:
at least one reservoir operable to receive a sample and fluidically connected to an imaging well comprising a detection area;
a growth well positioned between the at least one reservoir and the imaging well and containing growth media and at least one antibiotic;
signaling moieties and selection moieties stored in the at least one reservoir; and
a dye cushion reagent in the imaging well, the dye cushion reagent having a density agent and a dye that inhibits transmission of light.
2. The testing cartridge of claim 1 , wherein the density agent has a density greater than water.
3. The testing cartridge of claim 2 , wherein the density agent is selected from the group consisting of sucrose, diatrizoate, iodixanol, NaCl, CsCl, Percoll, metrizamide, and albumin.
4. The testing cartridge of claim 1 , wherein the signaling moieties and selection moieties specifically bind a target,
wherein the detection area is transparent at wavelengths corresponding to a signal signature of the signaling moieties, and
wherein the device comprising the imaging well comprises features for alignment or registration of the imaging well with an imagining analyzer.
5. The testing cartridge of claim 1 , wherein the longest linear dimension of the detection area is 2 cm, and wherein the depth of the imaging well is less than 2 cm.
6. The testing cartridge of claim 1 , wherein the selection moieties are magnetic particles.
7. The testing cartridge of claim 1 , wherein the signaling moieties are fluorescent particles.
8. The testing cartridge of claim 1 , wherein the signaling moieties are fluorescent or fluorogenic stains.
9. The testing cartridge of claim 1 , wherein the signaling moieties or selection moieties are stored in one of the one or more reservoirs in dry form.
10. The testing cartridge of claim 1 , wherein the imaging well has a depth of ≥2 mm and the detection area has a shortest linear dimension of ≥1 mm.
11. The testing cartridge of claim 1 , wherein the at least one reservoir is disposed above the imaging well.
12. The testing cartridge of claim 1 , comprising a second growth well positioned between the at least one reservoir and a second imaging well and containing growth media without antibiotic.