Insecticidal proteins and methods for their use
Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.
1. A recombinant PtIP-96 polypeptide comprising an amino acid sequence having at least 95% sequence identity to SEQ ID NO: 9, wherein the PtIP-96 polypeptide has insecticidal activity against Corn Earworm ( Helicoverpa zea ) and is operably linked to a heterologous transit or signal sequence.
2. The recombinant PtIP-96 polypeptide of claim 1 , wherein the PtIP-96 polypeptide has at least 98% sequence identity to the amino acid sequence of SEQ ID NO: 9.
3. A recombinant polynucleotide encoding a PtIP-96 polypeptide having at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 9, and having insecticidal activity against Corn Earworm, wherein the polynucleotide is operably linked to a heterologous regulatory element.
4. The recombinant polynucleotide of claim 3 , wherein the polynucleotide is a non-genomic polynucleotide.
5. The recombinant polynucleotide of claim 4 , wherein the polynucleotide is a cDNA.
6. The recombinant polynucleotide of claim 4 , wherein the polynucleotide is synthetic polynucleotide.
7. The recombinant polynucleotide of claim 6 , wherein the polynucleotide has codons optimized for expression in an agriculturally important crop.
8. A transgenic plant or plant cell comprising the polynucleotide of claim 3 .
9. A composition comprising the PtIP-96 polypeptide of claim 1 .
10. A method for controlling an insect pest population, comprising contacting the insect pest population with the PtIP-96 polypeptide of claim 1 .
11. A method of inhibiting growth or killing an insect pest, comprising contacting the insect pest with a composition comprising the PtIP-96 polypeptide of claim 1 .
12. A method of controlling Lepidoptera and/or Coleoptera insect infestation in a transgenic plant and providing insect resistance management, comprising expressing in the plant the PtIP-96 polypeptide of claim 1 .
13. A method for controlling an insect pest population, comprising contacting the insect pest population with the transgenic plant or plant cell of claim 8 .
14. A method of inhibiting growth or killing an insect pest, comprising transforming a plant with the recombinant polynucleotide of claim 3 .
15. The method of claim 14 , further comprising contacting the insect pest with the transgenic plant or plant cell.
16. The method of claim 14 , wherein the insect pest is Corn Earworm ( Helicoverpa zea ).
17. The method of claim 13 , 14 , 15 or 16 , wherein the insect pest or insect pest population is resistant to at least one Bt toxin.
18. The recombinant PtIP-96 polypeptide of claim 1 , wherein the PtIP-96 polypeptide is selected from SEQ ID NO: 9, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 18, SEQ ID NO: 20, SEQ ID NO: 22, and SEQ ID NO: 24.
19. The recombinant polynucleotide of claim 3 , wherein the encoded a PtIP-96 polypeptide has at least 98% sequence identity to the amino acid sequence of SEQ ID NO: 9.
20. The recombinant polynucleotide of claim 3 , wherein the encoded a PtIP-96 polypeptide is selected from SEQ ID NO: 9, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 18, SEQ ID NO: 20, SEQ ID NO: 22, and SEQ ID NO: 24.