Enzymatically produced cellulose
Enzymatic reactions are disclosed herein comprising water, glucose-1-phosphate, cellodextrin, and at least one cellodextrin phosphorylase enzyme comprising an amino acid sequence that is at least 90% identical to SEQ ID NO:2 or SEQ ID NO:6. These reactions produce a low molecular weight, insoluble cellulose with enhanced features.
1. An isolated composition comprising:
(i) insoluble cellulose having a weight-average degree of polymerization (DPw) of at least 15,
(ii) a cellodextrin phosphorylase enzyme comprising an amino acid sequence that is at least 90% identical to SEQ ID NO:2 or SEQ ID NO:6, and
(iii) a starch phosphorylase or sucrose phosphorylase.
2. The isolated composition of claim 1 , wherein the insoluble cellulose has a DPw of at least 17.
3. The isolated composition of claim 1 , wherein the insoluble cellulose has a DPw of 15 to 50.
4. The isolated composition of claim 1 , wherein the insoluble cellulose has a DPw of 15 to 30.
5. The isolated composition of claim 1 , wherein the insoluble cellulose has a DPw of 17 to 50.
6. The isolated composition of claim 1 , wherein the insoluble cellulose has a DPw of 17 to 30.
7. The isolated composition of claim 1 , wherein the cellodextrin phosphorylase enzyme comprises an amino acid sequence that is at least 95% identical to SEQ ID NO:2 or SEQ ID NO:6.
8. The isolated composition of claim 2 , wherein the cellodextrin phosphorylase enzyme comprises an amino acid sequence that is at least 95% identical to SEQ ID NO:2 or SEQ ID NO:6.
9. The isolated composition of claim 3 , wherein the cellodextrin phosphorylase enzyme comprises an amino acid sequence that is at least 95% identical to SEQ ID NO:2 or SEQ ID NO:6.
10. The isolated composition of claim 1 , wherein the isolated composition comprises the sucrose phosphorylase.
11. The isolated composition of claim 1 , wherein the isolated composition comprises the starch phosphorylase.
12. The isolated composition of claim 11 , wherein the isolated composition further comprises a starch debranching enzyme.
13. The isolated composition of claim 12 , wherein the starch debranching enzyme is a pullulanase or isoamylase.
14. The isolated composition of claim 1 , wherein the isolated composition is a food.
15. A method of producing insoluble cellulose in a composition, said method comprising:
combining, in a composition, at least water, cellodextrin, a cellodextrin phosphorylase enzyme, and (i) starch, inorganic phosphate and a starch phosphorylase, or (ii) sucrose, inorganic phosphate and a sucrose phosphorylase,
wherein the cellodextrin phosphorylase enzyme comprises an amino acid sequence that is at least 90% identical to SEQ ID NO:2 or SEQ ID NO:6, wherein insoluble cellulose having a weight-average degree of polymerization (DPw) of at least 15 is produced by the cellodextrin phosphorylase enzyme in the composition.
16. The method of claim 15 , wherein the cellodextrin comprises cellobiose.
17. The method of claim 15 , wherein the cellodextrin phosphorylase enzyme comprises an amino acid sequence that is at least 95% identical to SEQ ID NO:2 or SEQ ID NO:6.
18. The method of claim 15 , wherein the insoluble cellulose produced in the composition has a DPw of at least 17.
19. The method of claim 15 , wherein the insoluble cellulose produced in the composition has a DPw of 15 to 50.
20. The method of claim 15 , wherein the insoluble cellulose produced in the composition has a DPw of 17 to 50.
21. The method of claim 15 , wherein the composition is a food.