Haploid induction compositions and methods for use therefor
Provided here are methods of using a mutated patatin-like phospholipase IIα (“pPLAIIα,” renamed here MATRILINEAL) to induce haploid induction in plants, cloning a pPLAIIα to induce haploid induction in plants, and genetically engineering a plant to contain a mutated pPLAIIα. Also provided are methods of applying topical and spray chemicals, lipids, and RNAi molecules to plants during pollination in order to induce haploid production. Further provided are methods of chemically treating plants during pollination to induce haploids while also reducing embryo abortion and increasing seed set.
1. A plant containing a human-induced, non-transgenic knockout mutation within a gene encoding a patatin-like phospholipase, wherein the patatin-like phospholipase is selected from the group consisting of SEQ ID NO: 69, SEQ ID NO: 136, SEQ ID NO: 137, SEQ ID NO: 138, SEQ ID NO: 139, SEQ ID NO: 140, SEQ ID NO: 141, SEQ ID NO: 142, SEQ ID NO:143, and SEQ ID NO: 144.
2. The plant of claim 1 , wherein the human-induced, non-transgenic knockout mutation is an insertion of at least one nucleotide, a deletion of at least one nucleotide, or a substitution of at least one nucleotide, which creates a premature stop codon in the patatin-like phospholipase gene.
3. A seed, a pollen grain, a plant part, or the progeny of the plant of claim 1 ; wherein the seed, the pollen grain, the plant part, or the progeny comprises the mutation.
4. The plant of claim 2 , wherein the plant is selected from the group consisting of maize ( Zea mays ), sorghum ( Sorghum bicolor ), millet ( Setaria italica ), barley ( Hordeum vulgare ), stiff brome ( Brachypodium distachyon ), rice ( Oryza sativa ), wheat ( Triticum aestivum ), banana ( Musa acuminata ), palm ( Elaeis guineensis ), oats ( Avena sativa ), sunflower ( Helianthus annuus ), sugar beet ( Beta vulgaris ), Arabidopsis thaliana , and soybean ( Glycine max ).
5. The plant of claim 4 , wherein the plant is a maize plant.
6. The plant of claim 2 , wherein the patatin-like phosphase gene comprises SEQ ID NO: 68 or SEQ ID NO: 70.
7. The plant of claim 4 , wherein the plant is a rice plant.
8. The plant of claim 7 , wherein the patatin-like phosphase gene comprises SEQ ID NO: 84.