Cell culturing device
The invention features devices and kits for capturing and culturing microorganisms (e.g., bacteria, fungi, or protists) and methods of using the devices and kits to detect microorganisms in environmental and other samples. The device includes a nutrient media having a flat growth area on which microorganisms can grow. Samples are collected by contacting the device with any environmental sample, e.g., rolling device on a work surface or exposing device to air, or by filtering a sample through a membrane. Microorganisms deposited on the membrane derive nutrients from the underlying media and grow into colonies that can then be detected using methods known in the art. The detected colonies can be imaged digitally or with film.
1. A cassette comprising:
a circular base with a bottom surface and side wall;
a circular lid;
a circumferential rim integral with the circular lid or the circular base;
a plurality of detents, wherein, if the rim is on the circular lid, the plurality of detents are on a surface on the circular base, and vice versa, and whereby the circular lid and circular base are secured by inter-engagement between the circumferential rim and the plurality of detents resulting from axial compression; and
at least one centering detent, positioned to apply pressure on the circumferential rim, wherein
the plurality of detents are attached to the surface by a first ridge and the circumferential rim rests in a gap between the plurality of detents and the surface, and wherein the at least one centering detent is attached to a second ridge extending from the surface and distinct from the first ridge.
2. The cassette of claim 1 , wherein the plurality of detents are deflected as the circumferential rim passes the plurality of detents.
3. The cassette of claim 1 , wherein the plurality of detents are snap detents.
4. The cassette of claim 3 , wherein the plurality of snap detents have a pointed protrusion to increase force per area applied to the circumferential rim.
5. The cassette of claim 1 , wherein the at least one centering detent is in the circular base.
6. The cassette of claim 1 , further comprising an optically clear window in the circular lid.
7. The cassette of claim 1 , wherein the circular base contains a nutrient media for microorganisms.
8. The cassette of claim 1 , wherein the circumferential rim is continuous around the circular base or the circular lid.
9. A cassette comprising:
a circular base with a bottom surface and side wall;
a circular lid;
a circumferential rim, wherein the circumferential rim comprises at least one kerf; and
at least one ball detent and at least one secondary detent, wherein, if the rim is on the circular lid, the at least one ball detent and at least one secondary detent are on the circular base, and vice versa, whereby the circular lid and circular base are secured by inter-engagement between the circumferential rim and the at least one ball detent resulting from axial compression and whereby interaction between the at least one secondary detent and the kerf inhibits rotation of the lid relative to the base.
10. The cassette of claim 9 , further comprising at least one centering detent positioned to apply pressure on the circumferential rim.
11. The cassette of claim 9 , further comprising an optically clear window in the circular lid.
12. The cassette of claim 9 , wherein the circular base contains a nutrient media for microorganisms.