Methods for making recombinant proteins
The present invention provides methods and compositions for making proteins, preferably antibodies, more preferably anti-tumor necrosis factor alpha antibodies, and most preferably adalimumab. The present invention further provides methods and compositions for mammalian cell culture, preferably Chinese Hamster Ovary cells.
1. A liquid culture medium comprising:
about 0.1 to about 0.9 grams/liter glucose; and
at least one hexose selected from the group consisting of galactose, mannose, fructose, maltose, or a combination thereof, each at a concentration of about 0.1 to about 20 grams/liter.
2. The liquid culture medium of claim 1 , wherein the liquid culture medium is a chemically-defined liquid culture medium.
3. The liquid culture medium of claim 1 , wherein the liquid culture medium is an animal-derived component-free liquid culture medium.
4. The liquid culture medium of claim 1 , wherein the liquid culture medium is a serum-free liquid culture medium.
5. The liquid culture medium of claim 1 , wherein the liquid culture medium is a serum-containing liquid culture medium.
6. The liquid culture medium of claim 1 , wherein the liquid culture medium further comprises L-glutamine.
7. The liquid culture medium of claim 1 , wherein the liquid culture medium further comprises insulin growth factor 1 (IGF1).
8. The liquid culture medium of claim 1 , wherein the liquid culture medium further comprises L-glutamine and IGF1.
9. The liquid culture medium of claim 1 , wherein the liquid culture medium comprises at least one hexose selected from the group consisting of galactose, mannose, fructose, or a combination thereof, each at a concentration of about 0.1 g/L to about 20 g/L.
10. The liquid culture medium of claim 9 , wherein the liquid culture medium comprises galactose, mannose, and fructose, each at a concentration of about 0.1 g/L to about 20 g/L.
11. The liquid culture medium of claim 10 , wherein the liquid culture medium further comprises L-glutamine.
12. The liquid culture medium of claim 11 , wherein the liquid culture medium further comprises IGF1.
13. The liquid culture medium of claim 1 , wherein the liquid culture medium comprises mannose at a concentration of about 0.1 g/L to about 20 g/L.
14. The liquid culture medium of claim 13 , wherein the liquid culture medium further comprises L-glutamine.
15. The liquid culture medium of claim 14 , wherein the liquid culture medium further comprises IGF1.
16. The liquid culture medium of claim 1 , wherein the liquid culture medium comprises maltose at a concentration of 0.1 g/L to about 20 g/L.
17. The liquid culture medium of claim 16 , wherein the liquid culture medium further comprises L-glutamine.
18. The liquid culture medium of claim 1 , wherein the liquid culture medium comprises mannose and maltose, each at a concentration of about 0.1 g/L to about 20 g/L.
19. The liquid culture medium of claim 18 , wherein the liquid culture medium further comprises L-glutamine.
20. The liquid culture medium of claim 19 , wherein the liquid culture medium further comprises IGF1.