Transcriptional regulation for improved plant productivity
Methods comprising DNA constructs and polynucleotides of functional transcription factors for improving photosynthetic capacity, biomass and/or grain yield and stress tolerance in various crop and model plants, dicots and monocots with the C 3 or C 4 photosynthetic pathways are described herein.
1. A method for producing a genetically modified plant or plant cell having an increased carbon flow, the method comprising: a) transforming one or more host plants or plant cells with a vector comprising a nucleic acid encoding a polypeptide having at least 95% identity to SEQ ID NO: 4 operably linked to a promoter; and (b) selecting one or more transformed plants or plant cells for increased carbon flow by selecting for one or more of increased biomass yield, increased starch yield, increased glucose content, or increased sucrose content as compared to a control plant lacking the vector, wherein the promoter is light inducible.
2. The method according to claim 1 , the method further comprising collecting seeds comprising the vector from the selected plant.
3. The method according to claim 1 , the method further comprising regenerating a plant from the selected plant cell and collecting seeds comprising the vector from the regenerated plant.
4. The method of claim 1 , wherein the selected plant further exhibits increased tolerance to one or more abiotic stress factors of excess or deficiency of water and/or light, high or low temperature, or high salinity, as compared to a control plant lacking the vector.
5. The method of claim 1 , wherein the promoter comprises positions 8951 to 10645 of SEQ ID NO: 21.
6. The method of claim 1 , wherein the host plant is a monocotyledonous plant or a dicotyledonous plant.
7. The method of claim 6 , wherein the host plant is switchgrass.
8. The method of claim 6 , wherein the host plant is maize.
9. The method of claim 6 , wherein the host plant is sugar cane.
10. The method according to claim 1 , wherein the host plant is Miscanthus, Medicago , sweet sorghum, grain sorghum, sugar cane, energy cane, elephant grass, maize, wheat, barley, oat, rice, soybean, oil palm, safflower, sesame, flax, cotton, sunflower, Camelina, Brassica napus, Brassica carinata, Brassica juncea , pearl millet, or foxtail millet.
11. The method of claim 1 , wherein the polypeptide has at least 99% sequence identity to SEQ ID NO: 4.
12. The method of claim 11 , wherein the host plant is Miscanthus, Medicago , sweet sorghum, grain sorghum, sugar cane, energy cane, elephant grass, maize, wheat, barley, oat, rice, soybean, oil palm, safflower, sesame, flax, cotton, sunflower, Camelina, Brassica napus, Brassica carinata, Brassica juncea , pearl millet, or foxtail millet.
13. The method of claim 1 , wherein the polypeptide comprises SEQ ID NO: 4.
14. The method of claim 13 , wherein the host plant is Miscanthus, Medicago , sweet sorghum, grain sorghum, sugar cane, energy cane, elephant grass, maize, wheat, barley, oat, rice, soybean, oil palm, safflower, sesame, flax, cotton, sunflower, Camelina, Brassica napus, Brassica carinata, Brassica juncea , pearl millet, or foxtail millet.
15. The method according to claim 1 , wherein the nucleic acid comprises SEQ ID NO: 1.