Method for the fermentative production of L-lysine by modified
A method is used for the fermentative production of L-lysine using bacteria of the species Corynebacterium glutamicum , having the ability to excrete L-lysine and containing in their chromosome a mutated NCgl2816 polynucleotide. Further, the method is used for cultivating the bacteria in a suitable medium under suitable conditions, and accumulating said L-lysine in the suitable medium to form an L-lysine containing fermentation broth.
1. A method for the fermentative production of L-lysine, comprising:
a) providing a bacterium of the species Corynebacterium glutamicum having an ability to excrete L-lysine containing in the bacterium's chromosome a polynucleotide encoding a polypeptide comprising an amino acid sequence of SEQ ID NO:2, wherein an amino acid phenylalanine at position 220 of the amino acid sequence of SEQ ID NO:2 is substituted by a different proteinogenic amino acid,
b) cultivating the bacterium in a suitable medium under suitable conditions, and
c) accumulating said L-lysine in the medium to form an L-lysine containing fermentation broth.
2. The method of claim 1 , wherein, in the bacterium, the amino acid at position 220 of the amino acid sequence of SEQ ID NO:2 is cysteine.
3. The method of claim 2 , wherein, in the bacterium, the polynucleotide encoding said amino acid sequence comprises a nucleotide sequence of positions 343 to 1641 of SEQ ID NO:1 with nucleotides at positions 1000 to 1002 being tgt or tgc.
4. The method of claim 3 , wherein the nucleotides at positions 1000 to 1002 are tgc.
5. The method of claim 2 , wherein, in the bacterium, the polynucleotide encoding said amino acid sequence comprises a nucleotide sequence of positions 343 to 1644 of SEQ ID NO:1 with nucleotides at positions 1000 to 1002 being tgt or tgc.
6. The method of claim 5 , wherein the nucleotides at positions 1000 to 1002 are tgc.
7. The method of claim 2 , wherein, in the bacterium, the polynucleotide encoding said amino acid sequence comprises a nucleotide sequence of positions 221 to 1644 of SEQ ID NO:1 with nucleotides at positions 1000 to 1002 being tgt or tgc.
8. The method of claim 7 , wherein the nucleotides at positions 1000 to 1002 are tgc.
9. The method as claimed in claim 1 , further comprising manufacturing of an L-lysine containing product from the L-lysine containing fermentation broth.
10. The method as claimed in claim 1 , further comprising extracting or substantially eliminating water from the L-lysine containing fermentation broth.
11. The method of claim 9 , wherein said manufacturing comprises purification.