SAMPLE DESTRUCTION VALIDATION SYSTEM AND METHODS
Embodiments of the present invention allow for verifying the destruction of cells by another party. The destruction of cells can be verified by generating a value of an indicator (e.g., a bar code), where the value depends on some feature of the destruction. The party destroying the cells provides the value of the indicator as evidence that the cells have been destroyed. Embodiments may include a method of verifying the destruction of a cell. The method may include running a medium including a dead cell through an assay using a system. Running the medium through the assay may generate a value of an indicator to signify that the assay has been run. The value may identify the system, the cell, or the destruction mode of the cell. The method may further include receiving a verification code to acknowledge that the value is valid.
1 . A method of verifying the destruction of a cell, the method comprising:
running a medium including at least a fragment of a dead cell through an assay using a system, wherein:
running the medium through the assay generates a value of an indicator to signify that the assay has been run;
transmitting the value to a processor; and
receiving a verification code from the processor to acknowledge that value is valid.
2 . The method of claim 1 , wherein the value identifies the system, the cell, or the destruction mode of the cell.
3 . The method of claim 1 , further comprising destroying the cell using an agent to produce the medium including at least the fragment of the dead cell.
4 . The method of claim 1 , wherein the destruction of the cell comprises the cell dying from apoptosis.
5 . The method of claim 3 , wherein the agent comprises a lysing agent, ultraviolet radiation, an antiseptic, a cytotoxin, a virus, a bacteria, a fungus, heat, cold, a cytolysis agent, an acid, a base, a white blood cell, or high pressure.
6 . The method of claim 1 , wherein the cell comprises an NK-92 cell or a T cell.
7 . The method of claim 3 , wherein:
the value identifies the destruction mode of the cell, and
the presence of the agent generates the value of the indicator.
8 . The method of claim 1 , wherein the indicator is a bar code, a QR code, an image, an alphanumeric string, a character string, a number, a line, or a color.
9 . The method of claim 1 , wherein:
the cell is a type of cell,
the value of the indicator comprises a portion associated with the type of cell, and
the value of the indicator depends on a proportion or an amount of a compound in the medium.
10 . The method of claim 9 , wherein the compound comprises perforin, granzymes, or a component specific to cell death.
11 . The method of claim 9 , wherein the value of the indicator is generated when the proportion or the amount of the compound exceeds a threshold.
12 . The method of claim 1 , wherein:
the value identifies the system,
the system is labeled with an identifier, and
the value depends on the identifier.
13 . The method of claim 1 , wherein:
the value identifies the cell,
the cell is tagged with a chemical or genomic moiety, and
the value depends on the chemical or genomic moiety.
14 . The method of claim 1 , wherein the verification code comprises a blockchain portion.
15 . The method of claim 3 , wherein:
the agent comprises a first reactant,
the assay comprises using a second reactant,
the method further comprising:
reacting the first reactant and the second reactant to produce a product, and
detecting the product in the medium to generate the value of the indicator to signify that the assay has been run.
16 . The method of claim 1 , wherein:
the assay is a lateral flow test,
the system comprises a lateral flow test substrate,
the indicator is a bar code or QR code,
the value comprises an identifier associated with the identity of the lateral flow substrate, and
the value of the indicator is generated by the value becoming visible after running the medium through the assay.
17 . The method of claim 1 , wherein:
the value identifies the destruction mode of the cell,
the medium comprises a plurality of dead cells and a plurality of live cells, and
the value depends on a proportion of dead cells and live cells.
18 . The method of claim 1 , wherein transmitting the value comprises sending an image of the value.
19 . The method of claim 1 , wherein:
the indicator is a light, and the value is a color; or
the indicator is an electrical or magnetic characteristic.
20 . The method of claim 1 , wherein:
the assay is a bioassay, a ligand binding assay, an immunoassay, an enzyme assay, a light detection system, a radioisotope assay, a polymerase chain reaction assay, a photometry assay, a transmittance assay, a turbidimetry assay, a nephelometry assay, a reflectometry assay, a viscoelastic measurement, a counting assay, an imaging assay, a cytometry assay, or an electrical detection.