IP Library › Patent Application 16577923
Patent Application
App. No. 16/577,923

MEASUREMENT OF SERUM LIPOPROTEINS

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Patent No.
US None
App. No.
16/577,923
Abstract

Although a more accurate estimate of a person's risk of cardiovascular disease can be made on the basis of the number of lipoprotein particles per unit volume in the person's blood, current methods all rely on measuring the mass of lipoprotein cholesterol per unit volume. It has been discovered that a rapid and accurate lipoprotein particle count can be obtained by photometry. A method and apparatus are provided for measuring the number of lipoprotein particles in a sample using photometry.

Claims (36)

1 . A method of measuring lipoprotein particle count in a sample from a subject, the method comprising:

a. introducing into an apparatus for measuring the lipoprotein particle number, a plurality of lipoprotein fractions comprising a LDL fraction, a HDL fraction, and a VLDL fraction, wherein each of the plurality of lipoprotein fractions are separated from one another;

b. obtaining a photometric measurement in at least the LDL fraction; and

c. calculating a particle count for the LDL fraction that is a function of the photometric measurement,

wherein separation of the HDL fraction and the VLDL fraction is maintained during steps (a) to (c).

2 . The method of claim 1 further comprising:

a. obtaining a photometric measurement in the HDL fraction; and

b. calculating a particle count for the HDL fraction that is a function of the photometric measurement.

3 . The method of claim 1 further comprising:

a. obtaining a photometric measurement in at least the VLDL fraction; and

b. calculating a particle count for the VLDL fraction that is a function of the photometric measurement.

4 . The method of claim 1 further comprising:

a. obtaining a photometric measurement in at least the HDL fraction;

b. calculating a particle count for the HDL fraction that is a function of the photometric measurement;

c. obtaining a photometric measurement in at least the VLDL fraction; and

d. calculating a particle count for the VLDL fraction that is a function of the photometric measurement.

5 . The method of claim 1 , wherein the plurality of lipoprotein fractions are contained in a sample vessel and the LDL fraction, the HDL fraction and the VLDL fraction are introduced into the apparatus in a descending order of density.

6 . The method of claim 1 , wherein the sample is a blood sample or a serum sample.

7 . A method of measuring lipoprotein particle count in a sample from a subject, the method comprising:

a. introducing into an apparatus for measuring the lipoprotein particle number, a plurality of lipoprotein fractions comprising a LDL fraction, a HDL fraction, a Lp(a) fraction, an IDL fraction and a VLDL fraction, wherein each of the plurality of lipoprotein fractions are separated from one another;

b. obtaining a photometric measurement in at least one of the plurality of lipoprotein fractions fraction; and

c. calculating a particle count for each fraction for which the photometric measurement was obtained

wherein separation of the HDL fraction and the VLDL fraction is maintained during steps (a) to (c).

8 . The method of claim 7 , wherein the photometric measurement is obtained for the LDL fraction, the HDL fraction, the Lp(a) fraction, the IDL fraction, and the VLDL fraction.

9 . The method of claim 7 , wherein the at least one fraction is the LDL faction and the HDL fraction.

10 . The method of claim 7 , wherein the photometric measurement is obtained for the LDL fraction and the Lp(a) fraction.

11 . The method of claim 7 , wherein the photometric measurement is obtained for the LDL fraction and the IDL fraction.

12 . The method of claim 7 , wherein the photometric measurement is obtained for the LDL fraction and the VLDL fraction.

13 . The method of claim 7 , wherein the photometric measurement is obtained for the HDL fraction and the Lp(a) fraction.

14 . The method of claim 7 , wherein the photometric measurement is obtained for the HDL fraction and the IDL fraction.

15 . The method of claim 7 , wherein the photometric measurement is obtained for the HDL fraction and the VLDL fraction.

16 . The method of claim 7 , wherein the photometric measurement is obtained for the Lp(a) fraction and the VLDL fraction.

17 . The method of claim 7 , wherein the photometric measurement is obtained for the Lp(a) fraction and the IDL fraction.

18 . The method of claim 7 , wherein the photometric measurement is obtained for the VLDL fraction and the IDL fraction.

19 . The method of claim 7 , wherein the plurality of lipoprotein fractions are contained in a sample vessel and the LDL fraction, the HDL fraction, the Lp(a) fraction, the IDL fraction and the VLDL fraction are introduced into the apparatus in a descending order of density.

20 . The method of claim 7 , wherein the sample is a blood sample or a serum sample.