Method of measuring efficacy of treatment for an autoimmune disease in vivarium animals
A method of measuring efficacy of test treatment of an autoimmune disease in an animal in a vivarium is described. Animal activity data is collected at multiple times during the night. Sequential time regions of the night are identified as high-activity, activity-drop, or low-activity regions. Embodiments are described to quantify a drop, during the night, of an animal's activity level. These quantified activity-drop scalars for consecutive nights are accumulated in an animal health dataset. This dataset is compared to healthy animals, a standard of care or a reference treatment for the first disease to determine efficacy of the test treatment. One embodiment quantifies an activity-drop by fitting straight-line curves through the data in the three nightly regions. Other embodiment uses a Fourier transform on a circle, LASSO, RANSAC or regression analyses for curve fitting. Another embodiment compares areas under data curves in the regions. Animals may be housed in cages with other animals.
1. A method of measuring efficacy of a first treatment of an autoimmune disease in a study animal in a vivarium comprising the steps of:
(a) placing the study animal in a cage free of electronic penetrations in the vivarium and enrolling the study animal in a study;
(b) electronically observing one or more animal activities in real-time of the study animal using a combination of electronic cameras, infrared (IR) lighting of the study animals, and electronic hardware including computation and communication hardware;
(c) selecting a single “activity-drop metric” from the set: {regression analysis, LASSO, RANSAC, Monte Carlo} with an associated scalar “activity-drop value”;
(d) collecting a set of “nightly activity data” comprising values of the associated scalar activity drop value from electronically observing the study animal repeatedly and continually for a night;
(e) identifying automatically three consecutive time regions in the nightly activity data: a “high-activity region,” an “activity-drop region,” and a “low-activity region;”
(f) applying the activity-drop metric to the three consecutive regions, generating a nightly activity-drop value;
(g) adding the nightly activity-drop value into an “animal health dataset,” wherein the animal health dataset comprises the resulting nightly activity-drop values;
(h) iterating steps (b) through (g) for sequential nights until a terminating condition is reached;
wherein the study animal is housed in a home cage in the vivarium; and
wherein the collected data comprises the animal health dataset.
wherein the efficacy of the first treatment is a comparison of the animal health dataset to a reference treatment for the autoimmune disease;
(i) removing the study animal from the study when the terminating condition is reached.
2. The method of claim 1 further comprising:
(j) repeating steps (b) through (h) with multiple study animals in a first cohort;
(k) computing an average of the nightly activity-drop values, from step (f), for each night, for each of the multiple animals in the first cohort; wherein the each night is time shifted for each of the multiple animals in the cohort to align to an each reference night of each animal;
(l) adding the average of each night from step (k) into a cohort health dataset;
wherein the efficacy of the first treatment further includes comparison of the cohort health dataset to the reference treatment for the autoimmune disease.
3. The method of claim 2 wherein:
at least some of the multiple animals in the first cohort are housed in home cages in the vivarium wherein the home cages contain more than one animal; and
wherein the collecting step (d) of the animals is performed on animals in their home cage.