RNA formulation for immunotherapy
The present invention is in the field of immunotherapy, in particular tumor immunotherapy. The present invention provides pharmaceutical formulations for delivering RNA to antigen presenting cells such as dendrite cells (DCs) in the spleen after systemic administration. In particular, the formulations described herein enable to induce an immune response after systemic administration of antigen-coding RNA.
1. A pharmaceutical composition comprising nanoparticles that comprise:
(i) at least one cationic lipid, and
(ii) at least one RNA molecule encoding at least one antigen,
wherein, at physiological pH, an overall charge ratio of positive charges to negative charges of the nanoparticles is between 1:1.2 (0.83) and 1:2 (0.5), wherein the positive charges are contributed by the at least one cationic lipid, and the negative charges are contributed by the at least one RNA molecule;
a polydispersity index of the nanoparticles is 0.5 or less as measured by dynamic light scattering; and
the nanoparticles have an average diameter in the range of from about 250 nm to about 700 nm as measured by dynamic light scattering.
2. The pharmaceutical composition of claim 1 , wherein at physiological pH the overall charge ratio of positive charges to negative charges of the nanoparticles is between 1.6:2 (0.8) and 1:2 (0.5).
3. The pharmaceutical composition of claim 1 , wherein at physiological pH the overall charge ratio of positive charges to negative charges of the nanoparticles is between 1.6:2 (0.8) and 1.1:2 (0.55).
4. The pharmaceutical composition of claim 1 , wherein at physiological pH the overall charge ratio of positive charges to negative charges of the nanoparticles is 1.3:2 (0.65).
5. The pharmaceutical composition of claim 1 , wherein the polydispersity index of the nanoparticles is 0.4 or less as measured by dynamic light scattering.
6. The pharmaceutical composition of claim 1 , wherein the polydispersity index of the nanoparticles is 0.3 or less as measured by dynamic light scattering.
7. The pharmaceutical composition of claim 1 , wherein the nanoparticles have a zeta potential of from 0 mV to −50 mV.
8. The pharmaceutical composition of claim 1 , wherein the nanoparticles have a zeta potential of from −10 mV to −30 mV.
9. The pharmaceutical composition of claim 1 , wherein the nanoparticles have an average diameter in the range of from about 250 nm to about 550 nm as measured by dynamic light scattering.
10. The pharmaceutical composition of claim 1 , wherein the at least one cationic lipid comprises 1,2-di-O-octadecenyl-3-trimethylammonium propane (DOTMA) and/or 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP).
11. The pharmaceutical composition of claim 1 , wherein the at least one cationic lipid comprises DOTMA.
12. The pharmaceutical composition of claim 1 , wherein the at least one cationic lipid forms a complex with and/or encapsulates the at least one RNA molecule.
13. The pharmaceutical composition of claim 1 , wherein the at least one cationic lipid is comprised in a vesicle encapsulating the at least one RNA molecule.
14. The pharmaceutical composition of claim 1 , wherein the nanoparticles further comprise at least one helper lipid.
15. The pharmaceutical composition of claim 14 , wherein the at least one helper lipid is a neutral lipid.
16. The pharmaceutical composition of claim 14 , wherein the at least one helper lipid comprises 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine (DOPE), cholesterol (Chol) and/or 1,2-diolcoyl-sn-glycero-3-phosphocholine (DOPC).
17. The pharmaceutical composition of claim 14 , wherein the at least one helper lipid comprises DOPE.
18. The pharmaceutical composition of claim 14 , wherein a molar ratio of the at least one cationic lipid to the at least one helper lipid is from 9:1 to 3:7.
19. The pharmaceutical composition of claim 18 , wherein the molar ratio of the at least one cationic lipid to the at least one helper lipid is from 4:1 to 1:2, 4:1 to 2:3, 7:3 to 1:1, or 2:1 to 1:1.
20. The pharmaceutical composition of claim 18 , wherein the molar ratio of the at least one cationic lipid to the at least one helper lipid is about 2:1.
21. The pharmaceutical composition of claim 14 , wherein the at least one cationic lipid is DOTMA and the at least one helper lipid is DOPE.
22. The pharmaceutical composition of claim 21 , wherein the molar ratio of DOTMA to DOPE is about 2:1.
23. The pharmaceutical composition of claim 14 , wherein:
at physiological pH the overall charge ratio of positive charges to negative charges of the nanoparticles is between 1:1.2 (0.83) and 1:2 (0.5);
the nanoparticles have an average diameter in the range of from about 250 nm to about 550 nm as measured by dynamic light scattering; and
the molar ratio of the at least one cationic lipid to the at least one helper lipid is from 9:1 to 3:7.
24. The pharmaceutical composition of claim 23 , wherein:
at physiological pH the overall charge ratio of positive charges to negative charges of the nanoparticles is 1.3:2 (0.65);
the nanoparticles have an average diameter in the range of from about 300 nm to about 500 nm as measured by dynamic light scattering; and
the at least one cationic lipid is DOTMA and the at least one helper lipid is DOPE, and the molar ratio of DOTMA to DOPE is about 2:1.
25. The pharmaceutical composition of claim 1 , wherein the nanoparticles are lipoplexes comprising DOTMA and DOPE in a molar ratio of 8:2 to 3:7, and wherein the charge ratio of positive charges in DOTMA to negative charges in the at least one RNA molecule is 1.6:2 (0.8) to 1:2 (0.5).
26. The pharmaceutical composition of claim 25 , wherein the lipoplexes comprise DOTMA and DOPE in a molar ratio of 7:3 to 5:5 and wherein the charge ratio of positive charges in DOTMA to negative charges in the at least one RNA molecule is about 1.3:2 (0.65).
27. The pharmaceutical composition of claim 1 , wherein the nanoparticles are lipoplexes comprising DOTMA and Cholesterol in a molar ratio of 8:2 to 3:7, and wherein the charge ratio of positive charges in DOTMA to negative charges in the at least one RNA molecule is 1.6:2 (0.8) to 1:2 (0.5).
28. The pharmaceutical composition of claim 27 , wherein the lipoplexes comprise DOTMA and Cholesterol in a molar ratio of 7:3 to 5:5 and wherein the charge ratio of positive charges in DOTMA to negative charges in the at least one RNA molecule is about 1.3:2 (0.65).
29. The pharmaceutical composition of claim 1 , wherein the nanoparticles are lipoplexes comprising DOTAP and DOPE in a molar ratio of 8:2 to 3:7, and wherein the charge ratio of positive charges in DOTAP to negative charges in the at least one RNA molecule is 1.6:2 (0.8) to 1:2 (0.5).
30. The pharmaceutical composition of claim 29 , wherein the lipoplexes comprise DOTAP and DOPE in a molar ratio of 7:3 to 5:5 and wherein the charge ratio of positive charges in DOTAP to negative charges in the at least one RNA molecule is about 1.3:2 (0.65).
31. The pharmaceutical composition of claim 1 , wherein the nanoparticles are produced by a process comprising a step of incubating the at least one RNA molecule with bivalent cations prior to incorporation into said nanoparticles and/or by incubating the at least one RNA molecule with monovalent ions prior to incorporation into said nanoparticles and/or by incubating the at least one RNA molecule with one or more buffers prior to incorporation into said nanoparticles.
32. The pharmaceutical composition of claim 1 , wherein the at least one antigen is a disease-associated antigen or elicits an immune response against a disease-associated antigen or cells expressing a disease-associated antigen.
33. The pharmaceutical composition of claim 1 , wherein the at least one antigen is a tumor antigen or an antigen from an infectious agent.
34. The pharmaceutical composition of claim 1 , wherein the at least one RNA molecule is or comprises mRNA.
35. The pharmaceutical composition of claim 1 , wherein the at least one RNA molecule encoding at least one antigen comprises an unmasked poly-A sequence.
36. The pharmaceutical composition of claim 35 , wherein the unmasked poly-A sequence has a length of about 120 nucleotides.
37. The pharmaceutical composition of claim 1 , wherein the at least one RNA molecule encoding at least one antigen comprises a 3′UTR.
38. The pharmaceutical composition of claim 1 , wherein the at least one RNA molecule encoding at least one antigen further comprises a 5′-cap.
39. The pharmaceutical composition of claim 38 , wherein the 5′ cap comprises:
wherein:
R 1 and R 2 are each independently hydroxyl or methoxy; and
W, X, and Y are each independently selected form the group consisting of oxygen, sulfur, selenium and BH 3 .
40. The pharmaceutical composition of claim 39 , wherein:
(a) R 1 and R 2 are OH, and W, X, and Y are oxygen;
(b) one of R 1 and R 2 is OH, the other R 1 and R 2 is methoxy, and W, X, and Y are oxygen;
(c) R 1 and R 2 are OH, X is sulfur, and W and Y are oxygen; or
(d) R 2 is OH, R 1 is methoxy, one of W, X, and Y is sulfur, and the other two of W, X, and Y are oxygen.
41. The pharmaceutical composition of claim 39 , wherein R 2 is OH, R 1 is methoxy, X is sulfur, and W and Y are oxygen.
42. The pharmaceutical composition of claim 1 , wherein the pharmaceutical composition further comprises one or more pharmaceutically acceptable carriers, diluents, excipients, and/or adjuvants.
43. A method of treating or preventing a disease involving an antigen in a subject comprising administering to the subject the pharmaceutical composition of claim 1 , wherein the antigen is a disease-associated antigen or elicits an immune response against a disease-associated antigen or cells expressing a disease-associated antigen, whereby the disease in the subject is treated or the subject is prevented from developing the disease.
44. A pharmaceutical composition comprising nanoparticles that comprise:
(i) at least one cationic lipid,
(ii) at least one RNA molecule encoding at least one antigen, and
(iii) at least one helper lipid,
wherein, at physiological pH, an overall charge ratio of positive charges to negative charges of the nanoparticles is 1.3:2 (0.65), wherein the positive charges are contributed by the at least one cationic lipid, and the negative charges are contributed by the at least one RNA molecule;
a polydispersity index of the nanoparticles is 0.5 or less as measured by dynamic light scattering;
the nanoparticles have an average diameter in the range of from about 250 nm to about 700 nm as measured by dynamic light scattering;
the at least one cationic lipid is DOTMA;
the at least one helper lipid is DOPE; and
a molar ratio of DOTMA to DOPE is about 2:1.