Systems and methods for maintaining constant volumetric flow rates in a fluid channel
View Patent ↗Disclosed herein are systems and methods capable of identifying, tracking, and sorting particles flowing in a channel, for example, a microfluidic channel having a fluid medium. The channel and the fluid medium can have a similar refractive index such that they appear translucent or transparent when illuminated by electromagnetic radiation. The particles can have a refractive index substantially different from that of the channel and the medium, such that the particles interfere with the electromagnetic radiation. A sensor can be disposed adjacent to the channel to record the electromagnetic radiation. The sensor can be used for identifying, tracking, and sorting the particles.
1. A method of controlling a volumetric flow rate in a microfluidic particle sorting system, comprising:
(a) passing a medium containing a plurality of particles through a primary channel at a selected volumetric flow rate to a sensor to sense at least a subset of the particles;
(b) passing the medium with the sensed subset of particles to a first sorting junction comprising the primary channel connected to a first secondary channel and a second secondary channel;
(c) sorting the sensed subset of particles from the primary channel into the first secondary channel; and
(d) reinjecting a medium devoid of particles from the second secondary channel into the primary channel, wherein the reinjection of the medium from the second secondary channel maintains the selected volumetric flow rate in the primary channel.
2. The method of claim 1 , wherein the selected volumetric flow rate controls inter-particle spacing, inter-particle timing, particle positioning, or any combination thereof, within at least the primary channel.
3. The method of claim 2 , further comprising manipulating the inter-particle spacing, the inter-particle timing, and the particle positioning.
4. The method of claim 3 , wherein the manipulating comprises maintaining a constant inter-particle spacing and a constant inter-particle timing within the plurality of particles, varying the inter-particle spacing and inter-particle timing within the plurality of particles, grouping a subset of particles, agglomerating a subset of particles, or any combination thereof.
5. The method of claim 1 , wherein the selected volumetric flow rate controls inter-particle spacing, inter-particle timing, particle positioning, or any combination thereof, within at least the first secondary channel.
6. The method of claim 1 , wherein the sorting comprises dielectrophoretic manipulating and carrying by the medium.
7. The method of claim 6 , wherein the dielectrophoretic manipulating comprises inducing a dipole moment in the subset of the particles.
8. The method of claim 7 , wherein inducing the dipole moment in the subset of particles forces the subset of particles into the first secondary channel.
9. The method of claim 6 , wherein the carrying by the medium comprises employing an initial volumetric flow rate of the medium flowing into the first secondary channel with the subset of particles.
10. The method of claim 1 , wherein reinjecting the medium devoid of particles comprises reinjecting a volumetric flow rate of the medium devoid of particles equal to the volumetric flow rate of the medium flowing into the first secondary channel with the sensed subset of particles.