ANTIBODIES TO QUETIAPINE HAPTENS AND USE THEREOF
Disclosed is an antibody which binds to quetiapine, which can be used to detect quetiapine in a sample such as in a competitive immunoassay method. The antibody can be used in a lateral flow assay device for point-of-care detection of quetiapine, including multiplex detection of aripiprazole, olanzapine, quetiapine, and risperidone in a single lateral flow assay device.
1 - 14 . (canceled)
15 . An isolated antibody or a binding fragment thereof, which specifically binds to quetiapine, and is generated in response to a conjugate of a compound of Formula I:
wherein:
R 1 H,
R 2 H,
R 3 H,
m is 1, 2, 3, 4, or 5, and
n is 1, 2, 3, 4, or 5,
wherein either R 1 or R 2 is H, and both R 1 and R 2 may not simultaneously be H, and
wherein the immunogenic carrier is conjugated to the compound of Formula I at R 1 when R 1 is not H, or at R 2 when R 2 is not H.
16 . The isolated antibody or the binding fragment of claim 15 , wherein
R 1 H, or
and
R 2 is H, or
17 . The isolated antibody or the binding fragment of claim 15 , wherein R 1 is H, or
and
R 2 is H, or
18 . The isolated antibody or the binding fragment of claim 16 , wherein the compound of Formula I is selected from a group consisting of:
19 . The isolated antibody or the binding fragment of claim 15 , wherein the compound is N-((4-(Dibenzo[b,f][1,4]thiazepin-11-yl)-1-(2-(2-hydroxyethoxy)ethyl)piperazin-2-yl)methyl)-2-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)acetamide.
20 . The isolated antibody or the binding fragment of claim 15 , wherein the compound is 2-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)-N-((11-(4-(2-(2-hydroxyethoxy)ethyl)piperazin-1-yl)dibenzo[b,f][1,4]thiazepin-3-yl)methyl)acetamide.
21 . The isolated antibody or the binding fragment of claim 15 , wherein the immunogenic carrier is a protein.
22 . The isolated antibody or the binding fragment of claim 21 , wherein the protein is selected from a group consisting of keyhole limpet hemocyanin, bovine thyroglobulin, and ovalbumin.
23 . The isolated antibody or the binding fragment of claim 21 , wherein the immunogenic carrier is a thiol-activated protein.
24 . The isolated antibody or the binding fragment of claim 23 , wherein the thiol-activated protein comprises a nucleophilic sulfhydryl group.
25 . The isolated antibody or the binding fragment of claim 15 , wherein the conjugate is selected from a group consisting of:
N-((4-(dibenzo[b,f][1,4]thiazepin-11-yl)-1-(2-(2-hydroxyethoxy)ethyl)piperazin-2-yl)methyl)-2-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)acetamide-bovine thyroglobulin-conjugate,
2-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)-N-((11-(4-(2-(2-hydroxyethoxy)ethyl)piperazin-1-yl)dibenzo[b,f][1,4]thiazepin-3-yl)methyl)acetamide-bovine thyroglobulin-conjugate,
N-((4-(dibenzo[b,f][1,4]thiazepin-1-yl)-1-(2-(2-hydroxyethoxy)ethyl)piperazin-2-yl)methyl)-2-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)acetamide-keyhole limpet hemocyanin-conjugate, and
2-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)-N-((11-(4-(2-(2-hydroxyethoxy)ethyl)piperazin-1-yl)dibenzo[b,f][1,4]thiazepin-3-yl)methyl)acetamide-keyhole limpet hemocyanin-conjugate.
26 . The isolated antibody or the binding fragment of claim 15 , wherein the binding fragment is selected from the group of fragments consisting Fv, F(ab′), F(ab′)2, scFv, minibody and diabody fragments.
27 . The isolated antibody or the binding fragment of claim 15 , wherein the antibody is a monoclonal antibody.
28 . An assay kit comprising the antibody or the binding fragment thereof of claim 15 .
29 . An assay device comprising the antibody or the binding fragment thereof of claim 15 .
30 . The assay device of claim 25 , wherein the device is a lateral flow assay device.
31 . A method of detecting quetiapine in a sample comprising
(a) contacting a sample with the antibody or the binding fragment thereof of claim 15 labeled with a detectable marker, wherein the labeled antibody or the labeled binding fragment and quetiapine present in the sample form a labeled complex; and
(b) detecting the labeled complex so as to detect quetiapine in the sample.
32 . A competitive immunoassay method for detecting quetiapine in a sample comprising:
(a) contacting a sample with the antibody or the binding fragment thereof of claim 15 , and with quetiapine or a competitive binding partner of quetiapine, wherein one of the antibody or the binding fragment thereof and the quetiapine or the competitive binding partner thereof is labeled with a detectable marker, and wherein quetiapine in the sample competes with the quetiapine or competitive binding partner thereof for binding to the antibody or the binding fragment thereof; and
(b) detecting the label so as to detect quetiapine in the sample.
33 . The method of claim 32 , wherein the quetiapine or competitive binding partner thereof is labeled with the detectable marker.
34 . The method of claim 32 , wherein the antibody or the binding fragment thereof is labeled with a detectable marker.