Single use slurrying and chromatography systems
Methods and systems for chromatography are disclosed that employ a flexible container configured to fit within a support structure and adapted to receive a filtration or absorptive medium. The flexible container can include at least one inlet, at least one outlet, and a separation barrier peripherally sealed within the container to separate the container into a resin containing portion and a drainage portion. The barrier can be configured to exclude the resin material from the drainage portion while allowing fluids to pass therethrough. The disposable chromatography system can further include one or more agitators disposed within the flexible container and adjustably configured to be raised or lowered in the flexible container. When the agitator is in the raised position, the resin packing material can operate in a settled, packed-bed configuration. Alternatively, the agitator in the lowered position permits the chromatography resin packing material to operate in a mixed, slurry configuration.
1. A disposable container for chromatography comprising:
a flexible body configured to receive a chromatography resin, the flexible body comprising:
a separation barrier that separates the flexible body into a filtration portion and a drainage portion;
wherein the separation barrier retains the chromatography resin separately from the drainage portion during use while allowing fluids to pass therethrough.
2. The disposable container of claim 1 further comprising:
at least one agitator disposed within the flexible body and configured to be raised and lowered therein.
3. The disposable container of claim 2 , wherein the agitator in a raised position permits the chromatography resin to operate in a packed-bed configuration.
4. The disposable container of claim 2 , wherein the agitator in a lowered position permits the chromatography resin to operate in a slurry configuration.
5. The disposable container of claim 1 further comprising:
at least one probe coupled to the flexible body and operative to electrically communicate with a system controller.
6. The disposable container of claim 5 , wherein the at least one probe comprises: a temperature sensor, a pressure sensor, a UV sensor, a conductivity sensor, an optical density sensor, a pH sensor, or a turbidity sensor.
7. The disposable container of claim 1 , wherein the separation barrier is a manifold, a mesh, or a frit element; and
the chromatography resin comprises: a fibrous material, a flocculent, or a gel.
8. The disposable container of claim 1 further comprising:
a system controller coupled to the flexible body and configured to control at least one operational parameter of the disposable container.
9. The disposable container of claim 1 , wherein the disposable container is configured for use in: a batch process system, a drain and fill intermittent system, or a continuously operated perfusion system.
10. The disposable container of claim 1 further comprising:
a nozzle operative to fluidize the chromatography resin.
11. A method of performing chromatography comprising:
placing a disposable container within a support structure, the disposable container having a flexible body and a separation barrier for retaining a chromatography resin;
loading the flexible body with the chromatography resin;
filtering a liquid through the chromatography resin; and
releasing an eluate from the chromatography resin.
12. The method of claim 11 further comprising:
lowering an agitator into the flexible body during use to slurry the chromatography resin.
13. The method of claim 11 further comprising:
raising an agitator within the flexible body during use so that the chromatography resin forms a packed-bed.
14. The method of claim 11 further comprising:
delivering a gas to the separation barrier via at least one nozzle.
15. The method of claim 11 further comprising:
removing the disposable container from the support structure after use.
16. The method of claim 11 further comprising:
disposing of the disposable container after use.
17. The method of claim 11 , wherein the support structure forms part of a batch process system, a drain and fill intermittent system, or a continuously operated perfusion system.
18. The method of claim 11 further comprising:
fluidizing the chromatography resin via a nozzle.
19. A system for chromatography comprising:
a rigid support structure; and
a disposable container having a flexible body configured to be inserted into the rigid support structure and to receive a chromatography resin, the flexible body comprising:
a separation barrier that separates the flexible body into a filtration portion and a drainage portion;
wherein the separation barrier retains the chromatography resin from the drainage portion during use while allowing fluids to pass therethrough.
20. The system of claim 19 , wherein the support structure forms part of a batch process system, a drain and fill intermittent system, or a continuously operated perfusion system.