METHODS FOR INCREASING PLANT GROWTH AND YIELD BY USING AN ICTB SEQUENCE
Compositions and methods for increasing plant growth for higher crop yield are provided. The methods involve the expression of at least one ictB coding sequence in a C4 plant of interest. Further provided are methods for expressing at least one ictB coding sequence and increasing the expression of at least one additional coding sequence of interest selected from CO 2 assimilating sequences, such as those sequences involved in the Calvin Benson cycle, starch synthesis pathway, and C4 carbon shuttle in the plant. C4 plants expressing an ictB sequence are encompassed by the invention. Additionally, plants expressing an ictB sequence and those plants showing increased expression of a sequence of interest are encompassed by the invention. The expression of ictB in the C4 plant and the co-expression of ictB with this additional gene or genes results in yield gains.
1 . A composition comprising a promoter that is operable in a plant cell, operably linked to a coding sequence that encodes an ictB protein, wherein said ictB protein lacks a transit peptide.
2 . The composition of claim 1 , wherein said coding sequence shares at least 95% identity with the sequence set forth in SEQ ID NO:542, or a sequence that encodes an ictB protein that shares at least 95% identity with a sequence selected from the group consisting of SEQ ID NOs: 2, 34-68, and 536.
3 . A vector comprising the composition of claim 1 .
4 . A plant cell comprising the composition of claim 1 .
5 . A plant regenerated from the plant cell of claim 4 .
6 . Seed of the plant of claim 5 , wherein said seed comprises a promoter that is operable in a plant cell, wherein said promoter is operably linked to a coding sequence that encodes an ictB protein, wherein said ictB protein lacks a transit peptide.
7 . A plant having stably incorporated into its genome an ictB coding sequence operably linked to a promoter that is operable in plant cells, wherein said ictB coding sequence shares at least 95% identity with the sequence set forth in SEQ ID NO:542, or wherein said ictB coding sequence encodes an ictB protein sequence that shares at least 95% identity with a sequence selected from the group consisting of SEQ ID NOs: 2, 34-68, and 536, wherein said ictB coding sequence encodes an ictB protein that lacks a transit peptide.
8 . Seed of the plant of claim 7 , wherein said seed comprises an ictB coding sequence operably linked to a promoter that is operable in plant cells, wherein said ictB coding sequence shares at least 95% identity with the sequence set forth in SEQ ID NO:542, or wherein said ictB coding sequence encodes an ictB protein sequence that shares at least 95% identity with a sequence selected from the group consisting of SEQ ID NOs: 2, 34-68, and 536, wherein said ictB coding sequence encodes an ictB protein that lacks a transit peptide.
9 . The composition of claim 1 , wherein said coding sequence comprises the sequence set forth in SEQ ID NO:542, or a sequence that encodes an ictB protein that comprises a sequence selected from the group consisting of SEQ ID NOs: 2, 34-68, and 536.
10 . The plant of claim 7 , wherein said ictB coding sequence comprises the sequence set forth in SEQ ID NO:542, or wherein said ictB coding sequence encodes an ictB protein sequence that comprises a sequence selected from the group consisting of SEQ ID NOs: 2, 34-68, and 536, wherein said ictB coding sequence encodes an ictB protein that lacks a transit peptide.