Treatment of T-cell mediated diseases
The invention provides a method of treating T-cell mediated diseases and a method of inhibiting the activation of T-cells using certain diketopiperazines. The invention also provides methods of synthesizing diketopiperazines and pharmaceutical compositions comprising certain diketopiperazines. The invention further provides methods of making improved pharmaceutical compositions of proteins and peptides by either increasing or decreasing the content of diketopiperazines in the compositions and the resultant improved pharmaceutical compositions.
1. A method of manufacturing a pharmaceutical composition comprising passing a solution of albumin over an ultrafiltration membrane with a molecular weight cutoff that retains albumin to produce a filtrate, wherein the filtrate comprises an alanine-aspartic acid diketopiperazine (DA-DKP) and a component selected from the group consisting of caprylic acid, N-acetyl tryptophan and combinations thereof, wherein the composition is an isotonic aqueous solution.
2. The method of claim 1 wherein the filtrate contains components having molecular weights less than 3000.
3. The method of claim 1 wherein the albumin is a human albumin.
4. The method of claim 1 , further comprising placing the filtrate into a plastic bottle having a shaped closure adapted to deliver liquid contents dropwise.